[English] 日本語
Yorodumi- SASDE79: Interleukin-18 receptor 1, IL-18Rα-ECD (Interleukin-18 receptor 1) -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDE79 |
---|---|
Sample | Interleukin-18 receptor 1, IL-18Rα-ECD
|
Function / homology | Function and homology information interleukin-18 binding / interleukin-18 receptor activity / interleukin-18 receptor complex / interleukin-1 receptor activity / T-helper 1 cell differentiation / Interleukin-18 signaling / positive regulation of T-helper 1 cell cytokine production / interleukin-18-mediated signaling pathway / ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase / NAD+ nucleosidase activity ...interleukin-18 binding / interleukin-18 receptor activity / interleukin-18 receptor complex / interleukin-1 receptor activity / T-helper 1 cell differentiation / Interleukin-18 signaling / positive regulation of T-helper 1 cell cytokine production / interleukin-18-mediated signaling pathway / ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase / NAD+ nucleosidase activity / natural killer cell activation / NAD+ nucleotidase, cyclic ADP-ribose generating / Interleukin-37 signaling / negative regulation of cold-induced thermogenesis / positive regulation of non-canonical NF-kappaB signal transduction / positive regulation of type II interferon production / signaling receptor activity / positive regulation of NF-kappaB transcription factor activity / inflammatory response / immune response / signal transduction / plasma membrane Similarity search - Function |
Biological species | Homo sapiens (human) |
Citation | Journal: Structure / Year: 2019 Title: Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling. Authors: Jiwan Ge / Soumya G Remesh / Michal Hammel / Si Pan / Andrew D Mahan / Shuying Wang / Xinquan Wang / Abstract: The interleukin 1 (IL-1) receptor family, whose members contain three immunoglobulin-like domains (D1-D3) in the extracellular region, is responsible for transmitting pleiotropic signals of IL-1 ...The interleukin 1 (IL-1) receptor family, whose members contain three immunoglobulin-like domains (D1-D3) in the extracellular region, is responsible for transmitting pleiotropic signals of IL-1 cytokines. The inter-domain flexibility of IL-1 receptors and its functional roles have not been fully elucidated. In this study, we used small-angle X-ray scattering to show that ligand-binding primary receptors and co-receptors in the family all have inherent inter-domain flexibility due to the D2/D3 linker. Variants of the IL-1RAcP and IL-18Rβ co-receptors with mutated D2/D3 linkers cannot form a cytokine-receptor complex and mediate signaling. Our analysis further revealed that these mutated co-receptors exhibited a changed conformational ensemble, suggesting that loss of function is due to the alteration of receptor dynamics. Taken together, our results demonstrate that the D2/D3 linker is a critical functional determinant of IL-1 receptor and underscore the important roles of the inter-domain flexibility in cytokine/receptor binding and signaling. |
Contact author |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Data source
SASBDB page | SASDE79 |
---|
-Related structure data
Related structure data | C: citing same article (ref.) |
---|---|
Similar structure data |
-External links
Related items in Molecule of the Month |
---|
-Models
Model #2685 | Type: atomic / Chi-square value: 1.41925617646 Search similar-shape structures of this assembly by Omokage search (details) |
---|---|
Model #2686 | Type: atomic / Chi-square value: 1.41925617646 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Interleukin-18 receptor 1, IL-18Rα-ECD / Specimen concentration: 10 mg/ml |
---|---|
Buffer | Name: 10mM HEPES, 150mM NaCl, 3% glycerol / pH: 7.2 |
Entity #1395 | Type: protein / Description: Interleukin-18 receptor 1 / Formula weight: 36.302 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q13478 Sequence: CTSRPHITVV EGEPFYLKHC SCSLAHEIET TTKSWYKSSG SQEHVELNPR SSSRIALHDC VLEFWPVELN DTGSYFFQMK NYTQKWKLNV IRRNKHSCFT ERQVTSKIVE VKKFFQITCE NSYYQTLVNS TSLYKNCKKL LLENNKNPTI KKNAEFEDQG YYSCVHFLHH ...Sequence: CTSRPHITVV EGEPFYLKHC SCSLAHEIET TTKSWYKSSG SQEHVELNPR SSSRIALHDC VLEFWPVELN DTGSYFFQMK NYTQKWKLNV IRRNKHSCFT ERQVTSKIVE VKKFFQITCE NSYYQTLVNS TSLYKNCKKL LLENNKNPTI KKNAEFEDQG YYSCVHFLHH NGKLFNITKT FNITIVEDRS NIVPVLLGPK LNHVAVELGK NVRLNCSALL NEEDVIYWMF GEENGSDPNI HEEKEMRIMT PEGKWHASKV LRIENIGESN LNVLYNCTVA STGGTDTKSF ILVRKADMAD IPGHVFTRHH HHHH |
-Experimental information
Beam | Instrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS) City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.5 mm | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: MAR 165 CCD | ||||||||||||||||||||||||||||||
Scan | Title: Interleukin-18 receptor 1, IL-18Rα-ECD / Measurement date: Oct 3, 2014 / Cell temperature: 20 °C / Exposure time: 3 sec. / Number of frames: 600 / Unit: 1/A /
| ||||||||||||||||||||||||||||||
Distance distribution function P(R) | Sofotware P(R): GNOM 4.5a / Number of points: 385 /
| ||||||||||||||||||||||||||||||
Result | Type of curve: sec Comments: SEC-SAXS was performed at 20°C using the following parameters: Column: Schodex kw-803 ; Flow rate: 0.5 mL/min; Total acquisition time: 30min; Sample injection concentration: 10 mg/mL; Injection volume: 50 μL.
|