PDB-6axf: Structure of RasGRP2 in complex with Rap1B

Basic information

• Entry: Database: PDB / ID: 6axf
• Title: Structure of RasGRP2 in complex with Rap1B

Components

• RAS guanyl-releasing protein 2
• Ras-related protein Rap-1b

• Keywords: PROTEIN BINDING / SIGNALING PROTEIN

Function / homology

Function:

Rap protein signal transduction / diacylglycerol binding / modification of postsynaptic structure / regulation of cell junction assembly / Effects of PIP2 hydrolysis / negative regulation of calcium ion-dependent exocytosis / positive regulation of integrin activation / negative regulation of synaptic vesicle exocytosis / calcium-ion regulated exocytosis / Rap1 signalling / establishment of endothelial barrier / regulation of establishment of cell polarity / MET activates RAP1 and RAC1 / azurophil granule membrane / p130Cas linkage to MAPK signaling for integrins / GRB2:SOS provides linkage to MAPK signaling for Integrins / cellular response to cAMP / cellular response to calcium ion / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / Integrin signaling / lipid droplet / guanyl-nucleotide exchange factor activity / small monomeric GTPase / G protein activity / positive regulation of GTPase activity / establishment of localization in cell / regulation of cell growth / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / ruffle membrane / FCERI mediated NF-kB activation / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / GDP binding / Signaling by BRAF and RAF1 fusions / cell-cell junction / cell population proliferation / Ras protein signal transduction / positive regulation of ERK1 and ERK2 cascade / neuron projection / GTPase activity / glutamatergic synapse / lipid binding / synapse / calcium ion binding / Neutrophil degranulation / protein-containing complex binding / GTP binding / signal transduction / extracellular exosome / membrane / plasma membrane / cytosol

Domain/homology:

Ras-related protein Rap1 / RasGEF N-terminal motif / Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif / Ras-like guanine nucleotide exchange factor, N-terminal / Ras guanine-nucleotide exchange factors N-terminal domain profile. / Ras-like guanine nucleotide exchange factor / Ras guanine-nucleotide exchange factor, catalytic domain superfamily / Ras guanine nucleotide exchange factor domain superfamily / RasGEF domain / Ras guanine-nucleotide exchange factors catalytic domain profile. / Guanine nucleotide exchange factor for Ras-like small GTPases / Ras guanine-nucleotide exchange factors catalytic domain / Phorbol esters/diacylglycerol binding domain (C1 domain) / Zinc finger phorbol-ester/DAG-type signature. / Zinc finger phorbol-ester/DAG-type profile. / Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains) / Protein kinase C-like, phorbol ester/diacylglycerol-binding domain / C1-like domain superfamily / Small GTPase, Ras-type / small GTPase Ras family profile. / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / EF-hand domain pair / Rab subfamily of small GTPases / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Small GTP-binding protein domain / P-loop containing nucleotide triphosphate hydrolases / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta

Component:

Ras-related protein Rap-1b / RAS guanyl-releasing protein 2

• Biological species: Homo sapiens (human)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å
• Authors: Kondo, Y. / Iwig, J.S. / Kuriyan, J.
• Citation: Journal: Elife / Year: 2017; Title: A histidine pH sensor regulates activation of the Ras-specific guanine nucleotide exchange factor RasGRP1.; Authors: Vercoulen, Y. / Kondo, Y. / Iwig, J.S. / Janssen, A. / White, K.A. / Amini, M. / Barber, D.L. / Kuriyan, J. / Roose, J.P.

History

Deposition	Sep 6, 2017	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Oct 11, 2017	Provider: repository / Type: Initial release

Assembly

Deposited unit

A: RAS guanyl-releasing protein 2

B: Ras-related protein Rap-1b

C: RAS guanyl-releasing protein 2

D: Ras-related protein Rap-1b

E: RAS guanyl-releasing protein 2

F: Ras-related protein Rap-1b

G: RAS guanyl-releasing protein 2

H: Ras-related protein Rap-1b

I: RAS guanyl-releasing protein 2

J: Ras-related protein Rap-1b

K: RAS guanyl-releasing protein 2

L: Ras-related protein Rap-1b

M: RAS guanyl-releasing protein 2

N: Ras-related protein Rap-1b

O: RAS guanyl-releasing protein 2

P: Ras-related protein Rap-1b

Summary:

• 520 kDa, 16 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	520,239	16
Polymers	520,239	16
Non-polymers	0	0
Water	0	0

1

A: RAS guanyl-releasing protein 2

B: Ras-related protein Rap-1b

Summary:

• defined by author&software
• Evidence: gel filtration
• 65 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	65,030	2
Polymers	65,030	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3070 Å2
ΔGint	-18 kcal/mol
Surface area	25070 Å2
Method	PISA

2

C: RAS guanyl-releasing protein 2

D: Ras-related protein Rap-1b

Summary:

• defined by author&software
• Evidence: gel filtration
• 65 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	65,030	2
Polymers	65,030	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3170 Å2
ΔGint	-16 kcal/mol
Surface area	24790 Å2
Method	PISA

3

E: RAS guanyl-releasing protein 2

F: Ras-related protein Rap-1b

Summary:

• defined by author&software
• Evidence: gel filtration
• 65 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	65,030	2
Polymers	65,030	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3140 Å2
ΔGint	-18 kcal/mol
Surface area	24640 Å2
Method	PISA

4

G: RAS guanyl-releasing protein 2

H: Ras-related protein Rap-1b

Summary:

• defined by author&software
• Evidence: gel filtration
• 65 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	65,030	2
Polymers	65,030	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3090 Å2
ΔGint	-17 kcal/mol
Surface area	24580 Å2
Method	PISA

5

I: RAS guanyl-releasing protein 2

J: Ras-related protein Rap-1b

Summary:

• defined by author&software
• Evidence: gel filtration
• 65 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	65,030	2
Polymers	65,030	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3080 Å2
ΔGint	-17 kcal/mol
Surface area	23960 Å2
Method	PISA

6

K: RAS guanyl-releasing protein 2

L: Ras-related protein Rap-1b

Summary:

• defined by author&software
• Evidence: gel filtration
• 65 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	65,030	2
Polymers	65,030	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3160 Å2
ΔGint	-15 kcal/mol
Surface area	23730 Å2
Method	PISA

7

M: RAS guanyl-releasing protein 2

N: Ras-related protein Rap-1b

Summary:

• defined by author&software
• Evidence: gel filtration
• 65 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	65,030	2
Polymers	65,030	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3110 Å2
ΔGint	-19 kcal/mol
Surface area	24200 Å2
Method	PISA

8

O: RAS guanyl-releasing protein 2

P: Ras-related protein Rap-1b

Summary:

• defined by author&software
• Evidence: gel filtration
• 65 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	65,030	2
Polymers	65,030	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3070 Å2
ΔGint	-18 kcal/mol
Surface area	24050 Å2
Method	PISA

Unit cell

Length a, b, c (Å)	138.998, 209.258, 336.765
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	24
Space group name H-M	I212121

Components

#1: Protein

A C E G I K M O
RAS guanyl-releasing protein 2 / Calcium and DAG-regulated guanine nucleotide exchange factor I / CalDAG-GEFI / Cdc25-like protein / hCDC25L / F25B3.3 kinase-like protein

Details:

Mass: 45881.188 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: RASGRP2, CDC25L, MCG7 / Production host: Escherichia coli (E. coli) / References: UniProt: Q7LDG7

#2: Protein

B D F H J L N P
Ras-related protein Rap-1b / GTP-binding protein smg p21B

Details:

Mass: 19148.641 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: RAP1B, OK/SW-cl.11 / Production host: Escherichia coli (E. coli) / References: UniProt: P61224

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.35 ų/Da / Density % sol: 47.74 %
• Crystal grow: Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.6 / Details: 17% PEG3350 and 10 mM sodium citrate (pH 5.6)

Data collection

• Diffraction: Mean temperature: 100 K
• Diffraction source: Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 0.9795 Å
• Detector: Type: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 11, 2015
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.9795 Å / Relative weight: 1
• Reflection: Resolution: 3.1→49.46 Å / Num. obs: 171499 / % possible obs: 100 % / Redundancy: 4.5 % / CC_1/2: 0.993 / R_merge(I) obs: 0.148 / Net I/σ(I): 6.7

Processing

Software

Name	Version	Classification
PHENIX	(1.12_2829: ???)	refinement
XDS		data reduction
Aimless		data scaling
PHASER		phasing

Refinement

Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.1→49.46 Å / SU ML: 0.44 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 29.49 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection
Rfree	0.2577	4365	4.9 %
Rwork	0.2025	-	-
obs	0.2052	171493	99.61 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 3.1→49.46 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	34903	0	0	0	34903

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.003	35577
X-RAY DIFFRACTION	f_angle_d	0.55	48166
X-RAY DIFFRACTION	f_dihedral_angle_d	9.588	21645
X-RAY DIFFRACTION	f_chiral_restr	0.038	5473
X-RAY DIFFRACTION	f_plane_restr	0.004	6198

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
3.1-3.1352	0.3777	291	0.3305	5449	X-RAY DIFFRACTION	100
3.1352-3.1721	0.3559	271	0.3259	5446	X-RAY DIFFRACTION	100
3.1721-3.2108	0.4164	269	0.3264	5409	X-RAY DIFFRACTION	100
3.2108-3.2514	0.3571	291	0.3105	5441	X-RAY DIFFRACTION	100
3.2514-3.2942	0.3656	261	0.2971	5437	X-RAY DIFFRACTION	100
3.2942-3.3393	0.3238	270	0.2877	5528	X-RAY DIFFRACTION	100
3.3393-3.387	0.3429	228	0.2788	5524	X-RAY DIFFRACTION	100
3.387-3.4376	0.3442	301	0.2825	5388	X-RAY DIFFRACTION	100
3.4376-3.4913	0.2829	252	0.2582	5450	X-RAY DIFFRACTION	100
3.4913-3.5485	0.3267	292	0.2561	5435	X-RAY DIFFRACTION	100
3.5485-3.6096	0.3211	312	0.2304	5406	X-RAY DIFFRACTION	100
3.6096-3.6753	0.2834	247	0.2275	5525	X-RAY DIFFRACTION	100
3.6753-3.7459	0.2712	304	0.2169	5445	X-RAY DIFFRACTION	100
3.7459-3.8224	0.2669	269	0.2074	5444	X-RAY DIFFRACTION	100
3.8224-3.9054	0.2925	305	0.2101	5377	X-RAY DIFFRACTION	100
3.9054-3.9963	0.2564	313	0.2015	5444	X-RAY DIFFRACTION	100
3.9963-4.0961	0.2383	292	0.1921	5422	X-RAY DIFFRACTION	100
4.0961-4.2068	0.2154	243	0.1807	5514	X-RAY DIFFRACTION	100
4.2068-4.3306	0.2282	263	0.1759	5417	X-RAY DIFFRACTION	100
4.3306-4.4702	0.2306	287	0.1676	5467	X-RAY DIFFRACTION	100
4.4702-4.6299	0.2235	238	0.1669	5480	X-RAY DIFFRACTION	100
4.6299-4.8151	0.244	274	0.1621	5435	X-RAY DIFFRACTION	100
4.8151-5.0341	0.1882	278	0.1499	5453	X-RAY DIFFRACTION	100
5.0341-5.2992	0.2111	285	0.1717	5432	X-RAY DIFFRACTION	100
5.2992-5.6308	0.2643	281	0.183	5441	X-RAY DIFFRACTION	100
5.6308-6.0648	0.266	294	0.1942	5435	X-RAY DIFFRACTION	100
6.0648-6.6738	0.2631	314	0.2047	5387	X-RAY DIFFRACTION	100
6.6738-7.6365	0.2872	259	0.1834	5472	X-RAY DIFFRACTION	100
7.6365-9.6096	0.1881	303	0.1363	5432	X-RAY DIFFRACTION	100
9.6096-49.4665	0.1853	313	0.1601	5158	X-RAY DIFFRACTION	95

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.9472	-1.5203	0.7553	3.1548	0.5522	2.1753	-0.0317	1.0555	0.5013	-0.2209	0.063	-0.489	-0.1925	0.3082	0.0166	0.5606	-0.1262	0.0068	0.9719	0.214	0.7001	88.167	243.0602	-9.9381
2	1.3753	-0.4013	-0.229	2.3381	-2.18	2.084	0.0009	0.4434	0.0005	-0.3554	0.4678	0.1105	0.4139	-0.2517	-0.4497	1.1176	-0.1091	-0.0473	0.9865	-0.2804	0.7613	81.2385	215.5928	1.2026
3	1.519	0.174	0.4599	2.8078	-2.3393	3.2937	-0.0784	0.2122	0.0537	0.0963	0.0536	-0.2594	-0.0971	0.1099	0.0333	0.3997	0.0515	-0.0432	0.4218	-0.0877	0.4575	88.2641	223.3145	19.4409
4	3.1689	0.8524	0.7313	2.414	-0.7001	2.428	-0.0636	0.4609	-0.7021	-0.4942	0.1166	-0.0741	-0.2905	-0.6908	-0.1893	0.6556	0.006	-0.0867	0.5839	-0.0638	0.6241	79.4281	210.2715	22.203
5	2.1736	0.0744	-0.1208	1.8192	0.0089	2.6657	-0.1806	0.1835	0.4327	0.539	0.2697	-0.5344	-1.4023	-0.127	0.1082	1.1613	0.0883	-0.1968	0.4975	-0.1073	0.8427	87.9149	243.2533	29.1631
6	0.3629	-0.2474	-0.0762	2.4723	0.1113	2.6056	-0.3528	-0.138	0.0784	0.8532	0.6391	-0.3442	-0.8259	-0.2933	-0.0942	0.942	0.2592	-0.0564	0.5217	-0.0844	0.5318	81.9539	234.8596	33.3874
7	1.1845	0.285	0.718	2.4435	0.4824	0.5706	0.2164	-0.1632	0.1112	0.2821	-0.089	0.1748	-0.6323	-0.9636	-0.2842	1.3743	0.7068	-0.1521	0.8257	-0.3113	0.6568	75.9254	237.3926	38.2981
8	1.6796	-0.5507	0.6356	3.4365	-1.1147	2.3865	-0.6046	-0.9477	0.225	1.8685	0.7902	0.3429	-1.6754	-1.1463	-0.1098	1.6285	0.5782	0.0754	0.9087	-0.1057	0.7613	74.113	244.1432	39.8545
9	2.6764	0.8183	-0.72	2.8541	-0.2442	3.9269	-0.0796	0.5543	0.0432	-0.5053	-0.179	0.3583	0.407	-0.2593	0.1875	0.5523	-0.0733	-0.0225	0.6344	-0.0703	0.3638	50.8945	176.3942	-13.1712
10	2.4997	0.5096	0.7828	0.1279	0.4141	1.7379	-0.4774	1.0095	0.8439	-0.2038	0.1379	0.536	-0.787	1.1584	0.4903	1.1919	-0.0207	-0.2658	0.8891	0.0195	0.8701	58.1961	195.6348	-9.6104
11	3.8089	0.9291	0.0094	4.4869	1.0027	3.2171	-0.0675	-0.2674	0.6904	-0.1318	-0.2473	1.185	-0.9268	-0.3653	0.4304	0.7254	0.2281	-0.0088	0.5304	-0.0267	0.7088	52.9974	209.0129	15.1319
12	0.6997	0.2371	-0.155	4.7702	2.8521	4.1409	0.0643	-0.0611	0.1456	0.0578	-0.1774	0.4272	-0.0045	-0.4079	0.0849	0.3086	-0.0083	0.0325	0.4801	-0.0703	0.445	52.3796	194.1481	19.1694
13	2.9093	-2.5008	1.3464	5.4237	0.9908	6.3391	-0.3515	-1.1566	-0.0119	0.0982	0.0071	0.4746	-0.0998	-0.759	0.5116	0.7512	-0.041	0.009	0.75	0.084	0.3485	48.608	177.5132	34.7193
14	6.7125	0.2986	2.7134	0.4265	0.3148	4.2958	0.4829	0.4967	-0.8454	0.2843	0.1092	0.5046	1.0701	-0.1308	-0.6235	1.3074	-0.1144	0.1082	0.5876	0.1026	0.5555	51.2128	169.7148	21.1408
15	2.7666	-0.3945	1.0953	3.4659	-1.6284	3.0872	0.0859	-0.4987	0.423	1.2544	0.5769	1.1455	-0.9853	-1.4624	-1.2596	1.1341	-0.2857	0.2913	0.9878	0.1873	0.7398	44.9398	172.0007	32.765
16	0.8182	-0.6474	0.8209	2.1029	0.0557	1.3243	-0.1356	-0.2041	0.0505	1.3215	-0.5354	0.4159	0.555	-0.0855	0.6541	0.7264	0.0728	0.1066	0.6227	-0.1384	0.3533	55.8029	187.744	25.3702
17	1.3354	-0.0761	-0.0989	0.9881	-0.6612	0.7434	0.0497	-0.3752	0.501	0.0586	0.0588	0.0152	-0.0136	0.268	-0.0572	0.9192	-0.0493	0.0356	0.6787	0.011	0.4264	65.6031	181.147	31.8442
18	2.4786	-1.5164	0.0569	5.5335	0.3964	0.0796	-0.0331	-0.6736	-0.1057	0.6691	0.0351	0.0808	-0.1841	0.3251	-0.1131	1.075	0.072	0.0291	0.7284	0.0274	0.4143	60.4114	180.8368	38.617
19	4.4517	-0.0984	0.3471	2.5246	-2.2098	3.3114	-0.3375	-0.377	-0.4503	0.8977	-0.2778	-0.0029	0.2565	0.7333	0.8347	1.401	0.2441	-0.0306	0.9312	0.1868	0.5864	72.2194	164.7585	26.0585
20	3.2763	0.0781	-0.8832	2.5846	0.3115	1.7026	-0.8712	-0.342	-0.1276	1.0307	0.9101	-0.0816	0.0775	-0.0058	0.0571	1.5688	0.1507	0.0619	0.9347	0.0452	0.4956	66.8451	170.3826	36.4299
21	6.443	-0.3675	-0.1136	4.1268	0.1514	4.8065	-1.0901	-1.435	-1.1355	0.5639	0.3721	-0.3785	1.6731	0.1488	0.7857	1.2897	-0.185	0.099	0.8279	0.1025	0.5792	54.134	171.0469	39.2494
22	3.3897	-0.0635	0.5082	1.9265	-0.4617	2.9168	-0.2012	0.7487	-0.3462	-0.5254	0.3263	-0.4476	-0.3401	0.4826	-0.0338	0.7455	-0.2332	0.3794	0.9312	-0.2807	0.953	18.7471	241.8462	-15.4849
23	2.6222	1.3947	-1.3204	2.1879	-1.8418	5.1008	0.8209	0.5654	0.3845	-0.1374	0.0643	0.7042	0.6708	0.482	-0.7034	1.1462	-0.0149	0.2868	1.2268	-0.2867	0.9991	15.3055	228.127	-19.3998
24	1.3342	1.2524	-1.2968	2.1267	-0.8587	1.439	0.023	-0.4409	-0.2018	-0.4827	0.514	-1.531	0.3407	0.0089	-0.421	1.6527	-0.026	-0.5326	0.7928	-0.1203	1.4699	8.9053	205.6191	13.2626
25	1.4462	1.1715	0.8764	2.397	0.2245	3.5519	0.3995	-0.1669	-0.8671	0.1503	0.2675	-1.0494	0.6716	0.4866	-0.4342	0.4659	0.119	-0.1819	0.7452	-0.0674	1.3325	16.9096	223.4848	16.1303
26	2.9212	1.2948	2.3416	5.1433	-0.482	2.3895	-0.3179	-1.2273	0.1251	-0.1516	-0.0181	-0.4354	0.1604	0.8683	0.3563	0.176	-0.0088	-0.1612	1.6339	-0.3109	1.0119	20.3434	241.0428	32.2201
27	4.886	-0.3344	2.1295	2.5403	1.2056	4.9873	-0.5299	-0.2629	-0.0264	-0.0456	-0.2748	0.3435	-0.7382	-0.2856	0.585	0.8762	-0.0569	0.2622	0.7296	-0.1669	0.7408	12.0731	247.1517	21.7609
28	5.155	-2.0963	-1.7218	3.0788	3.3353	3.6697	0.6408	0.7114	0.5333	0.0463	-1.1566	0.3498	-1.4393	0.7322	0.3052	1.2315	-0.3097	0.2066	1.0604	-0.3201	0.9807	23.0234	250.3559	15.6301
29	4.9879	1.0082	-0.7656	1.7233	-2.5418	5.172	-0.0731	-0.8189	0.8442	0.8957	1.1065	-0.5565	-0.2952	1.5391	-0.9935	0.5335	0.1721	-0.0312	1.6105	-0.5163	1.0885	24.8628	247.9681	29.7139
30	2.1202	0.5534	0.106	3.3868	1.537	3.1382	-0.4612	-0.8924	-0.0785	0.2058	0.3555	-0.2187	-0.2999	0.4718	-0.1063	0.6001	-0.0511	-0.0005	1.0266	-0.3424	1.3649	17.9492	240.0607	25.6765
31	1.7942	-1.1567	-0.4453	1.5007	-0.9736	2.2788	-0.0915	-1.3457	-0.4377	-0.1543	-0.1484	-0.2038	-0.2609	0.8519	0.384	0.7926	0.0575	-0.2792	0.9231	-0.0536	1.3517	14.7222	229.1863	26.0246
32	2.0676	-1.1713	0.6724	2.3155	-0.0905	1.9705	-0.745	-0.2444	-0.3665	0.2036	0.1519	-0.4222	-0.0272	-0.8299	0.2247	0.4062	0.0516	0.004	0.9713	-0.0095	0.7433	3.4237	237.2007	29.313
33	2.4341	1.3618	-1.1436	5.3826	-1.7073	4.0597	0.1957	-0.535	-0.532	-0.2939	-0.8067	-0.4782	0.1167	-0.0727	1.0223	0.6805	-0.0822	0.058	1.1856	-0.1839	0.7984	8.456	237.5228	36.1654
34	3.6489	-1.5223	0.1571	1.7607	1.1888	1.4073	-0.5887	-0.1231	0.5048	-0.0276	-0.5783	0.6456	-0.5384	-0.8975	1.0385	0.9633	0.2978	-0.04	1.3216	-0.2474	0.8705	-3.3478	253.5427	23.4135
35	3.2836	-0.332	0.1851	5.5325	-0.5777	2.2919	0.5096	-0.8469	0.1414	0.5443	-0.1805	0.1783	-0.5152	-1.0024	-0.4348	0.9018	0.1602	0.0005	1.6265	-0.0231	0.8028	-3.5015	245.2566	37.0322
36	4.5194	0.876	-0.1643	2.2933	1.749	1.6076	0.297	-0.4864	0.2504	0.7294	0.7238	-0.4487	-0.7669	0.4586	-0.6497	0.9929	-0.2276	0.0673	1.2833	-0.3223	0.7777	5.5225	249.8469	31.7495
37	2.8288	0.5719	0.2806	0.5436	0.8768	3.7089	-0.5237	-1.8749	0.0889	0.2721	0.1171	-0.1559	-1.0623	-0.263	0.4292	0.4684	-0.2236	0.0162	1.2318	-0.2853	1.1967	14.981	248.047	35.9323
38	2.8496	-0.6455	-0.7713	3.489	-0.7605	3.3152	-0.1036	0.6874	-0.368	-0.5692	0.1976	0.2747	0.2925	0.1779	-0.0979	0.5461	-0.0529	-0.0503	0.6509	-0.1005	0.4131	-18.465	174.9507	-12.4123
39	1.3921	-0.9327	-1.0814	2.3121	1.2949	1.3066	0.251	0.4949	0.3235	0.0727	-0.1677	-0.0076	-0.0982	-0.6114	-0.2647	1.2557	0.031	-0.0519	0.8914	0.335	0.7602	-12.1971	201.4659	-2.6759
40	1.2589	0.003	-0.2603	3.8556	1.3131	3.4158	0.0319	0.0487	0.1752	0.0383	0.0634	0.1675	-0.5649	0.0382	-0.0948	0.5001	0.0747	0.0869	0.3563	0.0382	0.401	-17.5485	197.1352	16.8947
41	3.2221	-0.2794	-1.2809	2.1036	-0.4464	2.5034	-0.3739	-0.4381	-0.2177	-0.1256	0.3062	0.515	0.3912	0.1151	0.2438	0.7908	0.216	0.1659	0.469	0.1302	0.4587	-16.343	177.8259	30.0843
42	8.0978	0.4686	4.0416	0.2978	0.7519	2.0015	0.6367	0.1578	-1.3603	0.0817	0.5254	-0.138	1.4396	-1.5326	-0.4605	0.5181	0.0352	0.1915	0.4863	0.1719	1.1133	-23.6777	170.4999	19.5885
43	2.523	-0.0214	-0.1493	1.6701	-0.7343	0.917	-0.4019	0.1031	-0.197	0.5177	0.5575	0.3843	0.1969	-0.1706	0.0351	0.7608	0.1421	0.14	0.4471	0.0028	0.3954	-19.7185	182.5463	29.8573
44	3.3065	-1.0908	-1.3702	2.8784	1.357	1.8123	-0.5689	-0.9279	0.0639	0.5146	0.6738	0.1758	-0.4352	0.7993	-0.1358	0.6878	0.1707	-0.012	0.5805	0.0692	0.306	-5.0879	183.6226	33.1204
45	3.9808	-1.0823	-0.9372	0.9138	-0.7193	1.7012	-0.6523	-1.0642	-0.2687	0.6761	0.4144	0.0716	0.2387	0.6617	0.2131	0.9634	0.1972	0.0341	0.7899	0.1139	0.4488	-5.5143	174.4176	37.2363
46	5.3093	-0.4219	-1.4768	3.2691	0.9142	4.1516	0.2301	-0.8317	-0.1166	0.3009	-0.0542	-0.1723	0.3696	0.3693	-0.1285	0.4564	-0.0611	-0.051	0.617	0.0332	0.5191	-17.295	227.4539	96.7712
47	0.4625	0.0262	0.2569	0.2296	-0.2222	0.1652	0.1719	-0.3249	0.7329	-0.2894	0.2365	-0.0678	-0.5994	-0.023	-0.3225	1.6138	0.0609	-0.0423	1.0247	-0.1384	1.2276	-19.7463	249.7525	88.4634
48	2.483	-0.1891	-0.4382	2.0134	-0.6084	3.7251	0.315	0.0981	0.9165	-0.2803	0.0687	0.1821	-0.713	0.0529	-0.2599	0.6209	-0.0119	0.1788	0.3662	0.0145	0.8098	-15.6793	246.9122	65.3916
49	1.0477	0.9808	0.3539	0.8445	0.5366	1.5991	-0.1985	-0.0532	-0.0727	-0.085	-0.1528	-0.3751	0.2396	0.3435	0.2521	0.6971	0.1056	0.0063	0.5858	0.0676	0.7115	-15.9159	226.5684	54.106
50	6.3682	0.8585	-1.0855	0.8127	1.7147	5.174	0.7807	-0.1173	-1.5905	0.3852	-0.4148	0.0964	1.3725	1.1535	-0.3629	1.2872	0.2393	0.0749	0.4994	0.0404	0.802	-10.079	220.0699	65.645
51	1.4572	-0.6704	0.1947	0.408	0.4372	5.8767	0.3342	0.4178	0.4469	-0.3236	0.7537	-0.715	0.8428	1.5173	-1.2645	0.764	0.2163	0.2222	0.8526	-0.168	0.668	-6.9222	222.7912	51.996
52	4.2551	-0.5947	0.0823	2.8537	0.9366	3.0333	0.2008	0.2391	0.4483	-0.2924	0.2708	0.2356	0.4465	0.0721	-0.3692	0.4764	0.0146	0.0822	0.443	0.0008	0.5219	-13.9573	230.6803	55.563
53	0.2349	0.311	0.4593	0.5746	0.2809	1.1048	0.1357	0.0944	0.1283	-0.1767	-0.2378	-0.4003	-0.2835	-0.0429	0.1454	0.6355	0.1891	0.0631	0.5627	0.0439	0.6926	-21.2836	235.11	53.4204
54	4.4991	0.5821	-0.6768	1.7271	-0.6327	2.3906	-0.4258	0.7394	0.3906	-0.302	0.1913	-0.3497	-0.0294	-0.7755	0.1261	0.8921	0.1908	0.0226	0.6673	0.068	0.4765	-27.1819	233.3438	48.7505
55	3.5063	0.5123	-1.6111	2.1738	0.3085	2.5785	-0.184	1.3899	-0.169	-1.3418	0.074	-0.022	0.5722	-0.9388	-0.2088	1.0502	0.0436	-0.1364	0.9806	-0.1723	0.6385	-33.2269	224.3759	45.6912
56	8.9226	3.0376	-3.2246	6.4763	-0.8036	2.7278	-0.2241	0.668	-0.6279	-1.1335	0.4912	-0.2651	0.6858	0.3385	-0.203	0.7525	0.0871	0.0989	0.6642	-0.1434	0.4541	-18.6116	220.7885	47.2922
57	3.8266	0.494	-0.1736	3.6641	-1.7854	4.6484	0.2399	0.5074	-0.2121	-0.1544	-0.1308	0.1086	0.5094	-0.3814	-0.072	0.2666	0.0373	0.0062	0.4492	-0.1001	0.4638	13.9035	229.1326	74.2631
58	1.0466	0.8171	0.6127	2.8016	2.8952	2.734	0.1342	0.3236	0.5763	-0.478	-0.402	0.4608	-0.822	-0.5549	0.1645	1.1116	0.0682	-0.0312	0.6871	0.01	0.7904	20.558	252.4032	83.3639
59	2.2972	0.6041	-0.4139	3.3259	1.2499	3.1511	0.1882	-0.4663	0.5495	0.1256	-0.0165	0.324	-0.2937	0.058	-0.0748	0.3635	-0.0105	0.2091	0.4252	-0.1293	0.5708	19.6223	248.8518	104.9648
60	2.7111	-0.0764	0.1735	1.8779	-1.1946	1.5825	-0.5443	-0.2965	-0.2792	-0.1933	0.373	0.4427	0.7243	-0.3133	0.1761	0.5391	-0.0422	0.1314	0.6194	0.0067	0.4398	20.7492	228.561	116.5928
61	6.6042	0.2698	2.9037	0.0238	-0.1243	5.6269	1.0913	-1.0847	-0.5543	-0.0096	-0.1418	0.5962	1.0154	-0.5434	-0.7872	0.6251	-0.12	0.1763	0.5241	0.0509	0.7917	12.0644	222.2518	106.6052
62	1.5588	-0.1519	-0.2279	2.1569	-1.1403	2.3487	0.057	-0.5335	-0.0072	0.4284	-0.0792	0.6403	0.2343	-0.2248	0.0038	0.5779	-0.0807	0.1995	0.6391	-0.0249	0.5787	20.5694	232.7061	117.0195
63	2.8375	-0.4361	-0.1776	3.8137	-0.1839	1.4639	-0.2586	-0.8494	-0.0199	0.5524	0.2288	0.531	-0.366	0.2108	0.2513	0.5586	-0.0991	0.1132	0.6198	0.0286	0.3939	33.401	234.4758	119.1781
64	3.0777	0.4677	-0.6629	1.1483	-1.074	1.9754	-0.0338	-0.9573	-0.1748	0.5581	-0.0264	0.2929	0.0513	0.3863	-0.0869	0.7914	0.0518	0.1755	0.9265	0.1682	0.4634	32.1601	223.7994	122.0142
65	3.7556	-0.2486	-0.5502	2.9689	1.7013	4.9055	0.1723	-0.3719	-0.4023	0.2303	-0.1121	-0.1441	0.3815	0.0618	-0.0261	0.1849	-0.0745	-0.0166	0.2958	0.0847	0.2892	53.7366	227.1746	95.8357
66	3.3775	-1.518	2.2046	2.9547	-2.1322	2.4335	-0.058	-0.9929	0.7419	0.1849	-0.2039	-0.6125	-0.87	-0.7587	0.0471	1.3229	-0.0172	-0.0587	0.7733	-0.103	0.7877	49.0271	243.3004	97.6346
67	1.3126	-0.5141	0.4066	3.7613	-0.4023	2.7707	0.1214	-0.1372	0.3688	0.3347	0.0221	0.012	-1.1029	-0.0552	-0.1467	0.6284	0.014	0.1348	0.2604	-0.0523	0.4077	47.3977	252.0768	72.8106
68	1.267	-0.5978	0.2596	3.8851	-1.2276	3.3747	-0.0229	0.0445	0.2507	-0.2091	0.0686	-0.1637	-0.6532	-0.1206	-0.0365	0.3537	-0.0201	0.0466	0.3022	0.0132	0.4023	49.771	248.3829	64.0146
69	5.6219	-0.4586	-2.2425	3.2138	0.2821	5.3048	-0.238	0.2616	0.722	0.736	-0.2686	-0.279	0.7345	0.6008	0.0086	0.4368	-0.0389	0.0547	0.0428	-0.1458	0.5717	47.189	231.2936	51.4021
70	6.0475	1.6132	2.5982	1.4052	0.6085	4.6299	0.04	-0.2522	-0.964	-0.1261	0.0773	-0.8444	0.9466	0.4038	-0.2409	0.4785	0.096	-0.0488	0.3588	0.0073	0.4637	51.3776	222.2565	61.0495
71	1.089	0.1333	0.2223	2.442	1.5537	2.2551	-0.0307	0.1333	-0.1373	-0.6353	0.1417	-0.3537	-0.0988	0.627	-0.028	0.5577	0.0482	0.0979	0.4366	-0.02	0.4502	50.1221	233.0059	53.4492
72	2.9156	0.9215	-0.5425	3.3424	-0.0235	2.025	0.0334	0.5119	0.2282	-0.2669	0.0983	0.25	-0.4803	-0.5875	-0.1225	0.3674	0.1153	0.0205	0.3988	-0.0497	0.2953	35.4154	235.5744	51.1502
73	6.157	-1.5424	2.0541	4.8592	0.1419	5.312	0.43	-0.1865	-0.648	0.2104	-0.1851	0.6535	1.4307	-0.0582	0.0214	0.6424	-0.1035	0.1364	0.4828	0.0993	0.4016	27.8944	218.9328	58.5006
74	3.7282	-0.7773	0.1464	4.749	-0.9267	2.5866	-0.2582	0.3333	0.0375	-0.9197	0.5272	-0.2141	0.0725	-0.4283	-0.353	0.6776	-0.0541	0.0069	0.5842	-0.1409	0.3507	32.1592	225.1054	48.0817
75	3.3913	2.0427	-2.4549	4.7221	-0.7081	3.6891	-0.4703	0.5788	-0.4359	-1.1812	0.3653	0.3503	0.6128	-1.1692	0.1664	0.4452	-0.0092	0.0593	0.5394	-0.1186	0.3883	44.389	225.7835	43.8813
76	3.5887	-0.8801	1.0026	4.2741	-0.3707	3.8877	0.1322	-0.6094	0.0865	0.7843	-0.3601	0.3292	0.1237	-0.6809	0.1535	0.4258	-0.0963	0.113	0.6345	-0.0567	0.4785	87.3765	191.3112	99.6927
77	0.4139	-0.0343	-0.5046	1.1204	1.4009	1.6468	0.1454	-0.2225	-0.1149	-0.1856	0.3818	-0.062	-0.1197	1.2422	-0.2853	1.502	-0.028	0.205	0.9859	0.1556	0.6343	90.4469	165.9903	87.1848
78	1.4093	0.0023	0.4423	4.2056	0.454	2.9536	0.2484	-0.0307	-0.2611	-0.1065	-0.232	-0.025	0.5965	0.0047	-0.038	0.3403	0.0201	0.021	0.4237	0.0527	0.4641	85.374	171.4935	68.579
79	3.0271	0.6587	-0.0491	1.7816	0.1052	2.9357	-0.3201	-0.4614	-0.0237	0.1607	0.1527	0.4858	-0.5564	-0.0881	0.2596	0.4819	0.0938	0.0622	0.4357	0.0521	0.5175	83.7555	194.1808	61.3844
80	2.6195	-0.2876	-0.8085	2.3981	-1.3515	1.0516	0.0214	-0.103	0.0602	-0.4395	0.3504	0.3633	0.2482	-0.5168	-0.3862	0.4409	-0.022	-0.0445	0.4888	0.0414	0.4258	82.3185	187.2985	57.4323
81	3.2081	0.9498	0.9286	2.2341	0.4704	1.8651	-0.2066	0.3927	-0.6766	-0.1738	0.3153	-0.395	0.1969	0.5398	-0.1381	0.4895	0.1298	0.0109	0.4971	0.0252	0.5051	96.1363	184.5563	51.7914
82	3.654	-0.8182	0.307	3.6637	0.1096	3.9662	0.0451	0.1904	0.8054	-0.4247	-0.0106	-0.4149	-0.6715	0.954	-0.1764	0.33	0.0823	0.0854	0.865	0.1589	0.731	101.1038	196.6557	51.6416
83	7.9135	1.4166	3.0377	6.2241	-0.0386	7.9078	-0.3038	1.7739	0.8432	-0.9482	0.5108	0.1085	-0.4719	0.2721	-0.31	0.3543	-0.1038	0.1112	0.5878	0.1108	0.3691	85.7668	194.7494	46.9676

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 8 through 110 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 111 through 154 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 155 through 349 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 350 through 387 )
5	X-RAY DIFFRACTION	5	chain 'B' and (resid 1 through 36 )
6	X-RAY DIFFRACTION	6	chain 'B' and (resid 37 through 103 )
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 104 through 120 )
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 121 through 167 )
9	X-RAY DIFFRACTION	9	chain 'C' and (resid 7 through 109 )
10	X-RAY DIFFRACTION	10	chain 'C' and (resid 110 through 141 )
11	X-RAY DIFFRACTION	11	chain 'C' and (resid 142 through 172 )
12	X-RAY DIFFRACTION	12	chain 'C' and (resid 173 through 387 )
13	X-RAY DIFFRACTION	13	chain 'D' and (resid 1 through 10 )
14	X-RAY DIFFRACTION	14	chain 'D' and (resid 11 through 36 )
15	X-RAY DIFFRACTION	15	chain 'D' and (resid 37 through 57 )
16	X-RAY DIFFRACTION	16	chain 'D' and (resid 58 through 74 )
17	X-RAY DIFFRACTION	17	chain 'D' and (resid 75 through 103 )
18	X-RAY DIFFRACTION	18	chain 'D' and (resid 104 through 116 )
19	X-RAY DIFFRACTION	19	chain 'D' and (resid 117 through 127 )
20	X-RAY DIFFRACTION	20	chain 'D' and (resid 128 through 153 )
21	X-RAY DIFFRACTION	21	chain 'D' and (resid 154 through 167 )
22	X-RAY DIFFRACTION	22	chain 'E' and (resid 7 through 109 )
23	X-RAY DIFFRACTION	23	chain 'E' and (resid 110 through 132 )
24	X-RAY DIFFRACTION	24	chain 'E' and (resid 133 through 154 )
25	X-RAY DIFFRACTION	25	chain 'E' and (resid 155 through 387 )
26	X-RAY DIFFRACTION	26	chain 'F' and (resid 1 through 10 )
27	X-RAY DIFFRACTION	27	chain 'F' and (resid 11 through 24 )
28	X-RAY DIFFRACTION	28	chain 'F' and (resid 25 through 36 )
29	X-RAY DIFFRACTION	29	chain 'F' and (resid 37 through 48 )
30	X-RAY DIFFRACTION	30	chain 'F' and (resid 49 through 64 )
31	X-RAY DIFFRACTION	31	chain 'F' and (resid 65 through 74 )
32	X-RAY DIFFRACTION	32	chain 'F' and (resid 75 through 103 )
33	X-RAY DIFFRACTION	33	chain 'F' and (resid 104 through 116 )
34	X-RAY DIFFRACTION	34	chain 'F' and (resid 117 through 127 )
35	X-RAY DIFFRACTION	35	chain 'F' and (resid 128 through 137 )
36	X-RAY DIFFRACTION	36	chain 'F' and (resid 138 through 153 )
37	X-RAY DIFFRACTION	37	chain 'F' and (resid 154 through 166 )
38	X-RAY DIFFRACTION	38	chain 'G' and (resid 7 through 109 )
39	X-RAY DIFFRACTION	39	chain 'G' and (resid 110 through 154 )
40	X-RAY DIFFRACTION	40	chain 'G' and (resid 155 through 387 )
41	X-RAY DIFFRACTION	41	chain 'H' and (resid 1 through 24 )
42	X-RAY DIFFRACTION	42	chain 'H' and (resid 25 through 36 )
43	X-RAY DIFFRACTION	43	chain 'H' and (resid 37 through 74 )
44	X-RAY DIFFRACTION	44	chain 'H' and (resid 75 through 120 )
45	X-RAY DIFFRACTION	45	chain 'H' and (resid 121 through 167 )
46	X-RAY DIFFRACTION	46	chain 'I' and (resid 7 through 109 )
47	X-RAY DIFFRACTION	47	chain 'I' and (resid 110 through 154 )
48	X-RAY DIFFRACTION	48	chain 'I' and (resid 155 through 387 )
49	X-RAY DIFFRACTION	49	chain 'J' and (resid 1 through 24 )
50	X-RAY DIFFRACTION	50	chain 'J' and (resid 25 through 36 )
51	X-RAY DIFFRACTION	51	chain 'J' and (resid 37 through 48 )
52	X-RAY DIFFRACTION	52	chain 'J' and (resid 49 through 64 )
53	X-RAY DIFFRACTION	53	chain 'J' and (resid 65 through 86 )
54	X-RAY DIFFRACTION	54	chain 'J' and (resid 87 through 120 )
55	X-RAY DIFFRACTION	55	chain 'J' and (resid 121 through 145 )
56	X-RAY DIFFRACTION	56	chain 'J' and (resid 146 through 167 )
57	X-RAY DIFFRACTION	57	chain 'K' and (resid 8 through 110 )
58	X-RAY DIFFRACTION	58	chain 'K' and (resid 111 through 154 )
59	X-RAY DIFFRACTION	59	chain 'K' and (resid 155 through 387 )
60	X-RAY DIFFRACTION	60	chain 'L' and (resid 1 through 24 )
61	X-RAY DIFFRACTION	61	chain 'L' and (resid 25 through 36 )
62	X-RAY DIFFRACTION	62	chain 'L' and (resid 37 through 86 )
63	X-RAY DIFFRACTION	63	chain 'L' and (resid 87 through 120 )
64	X-RAY DIFFRACTION	64	chain 'L' and (resid 121 through 167 )
65	X-RAY DIFFRACTION	65	chain 'M' and (resid 6 through 109 )
66	X-RAY DIFFRACTION	66	chain 'M' and (resid 110 through 143 )
67	X-RAY DIFFRACTION	67	chain 'M' and (resid 144 through 190 )
68	X-RAY DIFFRACTION	68	chain 'M' and (resid 191 through 387 )
69	X-RAY DIFFRACTION	69	chain 'N' and (resid 1 through 15 )
70	X-RAY DIFFRACTION	70	chain 'N' and (resid 16 through 36 )
71	X-RAY DIFFRACTION	71	chain 'N' and (resid 37 through 74 )
72	X-RAY DIFFRACTION	72	chain 'N' and (resid 75 through 116 )
73	X-RAY DIFFRACTION	73	chain 'N' and (resid 117 through 127 )
74	X-RAY DIFFRACTION	74	chain 'N' and (resid 128 through 153 )
75	X-RAY DIFFRACTION	75	chain 'N' and (resid 154 through 167 )
76	X-RAY DIFFRACTION	76	chain 'O' and (resid 7 through 109 )
77	X-RAY DIFFRACTION	77	chain 'O' and (resid 110 through 154 )
78	X-RAY DIFFRACTION	78	chain 'O' and (resid 155 through 387 )
79	X-RAY DIFFRACTION	79	chain 'P' and (resid 1 through 36 )
80	X-RAY DIFFRACTION	80	chain 'P' and (resid 37 through 74 )
81	X-RAY DIFFRACTION	81	chain 'P' and (resid 75 through 116 )
82	X-RAY DIFFRACTION	82	chain 'P' and (resid 117 through 153 )
83	X-RAY DIFFRACTION	83	chain 'P' and (resid 154 through 167 )