A: RAS guanyl-releasing protein 2 B: Ras-related protein Rap-1b C: RAS guanyl-releasing protein 2 D: Ras-related protein Rap-1b E: RAS guanyl-releasing protein 2 F: Ras-related protein Rap-1b G: RAS guanyl-releasing protein 2 H: Ras-related protein Rap-1b I: RAS guanyl-releasing protein 2 J: Ras-related protein Rap-1b K: RAS guanyl-releasing protein 2 L: Ras-related protein Rap-1b M: RAS guanyl-releasing protein 2 N: Ras-related protein Rap-1b O: RAS guanyl-releasing protein 2 P: Ras-related protein Rap-1b
RASguanyl-releasingprotein2 / Calcium and DAG-regulated guanine nucleotide exchange factor I / CalDAG-GEFI / Cdc25-like protein / ...Calcium and DAG-regulated guanine nucleotide exchange factor I / CalDAG-GEFI / Cdc25-like protein / hCDC25L / F25B3.3 kinase-like protein
Mass: 45881.188 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RASGRP2, CDC25L, MCG7 / Production host: Escherichia coli (E. coli) / References: UniProt: Q7LDG7
#2: Protein
Ras-relatedproteinRap-1b / GTP-binding protein smg p21B
Mass: 19148.641 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RAP1B, OK/SW-cl.11 / Production host: Escherichia coli (E. coli) / References: UniProt: P61224
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.35 Å3/Da / Density % sol: 47.74 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.6 / Details: 17% PEG3350 and 10 mM sodium citrate (pH 5.6)
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 0.9795 Å