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- PDB-6wpf: Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsD... -

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Basic information

Entry
Database: PDB / ID: 6wpf
TitleStructure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and d4T
Components
  • DNA Primer 21-mer
  • DNA template 27-mer
  • Reverse transcriptase/ribonuclease H
  • p51 RT
KeywordsTRANSFERASE/DNA / Reverse Transcriptase / ternary complex / d4T / chain terminator / stereochemistry / TRANSFERASE / TRANSFERASE-DNA complex / Stavudine
Function / homology
Function and homology information


integrase activity / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / Minus-strand DNA synthesis / Plus-strand DNA synthesis / Uncoating of the HIV Virion / 2-LTR circle formation / Vpr-mediated nuclear import of PICs / Early Phase of HIV Life Cycle / Integration of provirus ...integrase activity / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / Minus-strand DNA synthesis / Plus-strand DNA synthesis / Uncoating of the HIV Virion / 2-LTR circle formation / Vpr-mediated nuclear import of PICs / Early Phase of HIV Life Cycle / Integration of provirus / APOBEC3G mediated resistance to HIV-1 infection / Binding and entry of HIV virion / viral life cycle / HIV-1 retropepsin / symbiont-mediated activation of host apoptosis / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / Assembly Of The HIV Virion / Budding and maturation of HIV virion / host multivesicular body / protein processing / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / viral penetration into host nucleus / RNA stem-loop binding / RNA-directed DNA polymerase activity / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / peptidase activity / host cell / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / aspartic-type endopeptidase activity / Hydrolases; Acting on ester bonds / DNA-directed DNA polymerase activity / symbiont-mediated suppression of host gene expression / viral translational frameshifting / lipid binding / symbiont entry into host cell / host cell nucleus / host cell plasma membrane / virion membrane / structural molecule activity / DNA binding / zinc ion binding / identical protein binding / membrane
Similarity search - Function
Reverse transcriptase connection / Reverse transcriptase connection domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral / Integrase, N-terminal zinc-binding domain ...Reverse transcriptase connection / Reverse transcriptase connection domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral / Integrase, N-terminal zinc-binding domain / Integrase, C-terminal, retroviral / Integrase DNA binding domain profile. / Immunodeficiency lentiviral matrix, N-terminal / gag gene protein p17 (matrix protein) / RNase H / Integrase core domain / Integrase, catalytic core / Integrase catalytic domain profile. / Retropepsin-like catalytic domain / Matrix protein, lentiviral and alpha-retroviral, N-terminal / Retroviral nucleocapsid Gag protein p24, C-terminal domain / Gag protein p24 C-terminal domain / RNase H type-1 domain profile. / Ribonuclease H domain / Retropepsins / Retroviral aspartyl protease / Aspartyl protease, retroviral-type family profile. / Peptidase A2A, retrovirus, catalytic / Reverse transcriptase domain / Reverse transcriptase (RNA-dependent DNA polymerase) / Reverse transcriptase (RT) catalytic domain profile. / Retrovirus capsid, C-terminal / Retroviral matrix protein / Retrovirus capsid, N-terminal / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Aspartic peptidase, active site / Eukaryotic and viral aspartyl proteases active site. / Aspartic peptidase domain superfamily / Ribonuclease H superfamily / Ribonuclease H-like superfamily / Reverse transcriptase/Diguanylate cyclase domain / DNA/RNA polymerase superfamily
Similarity search - Domain/homology
Chem-D4M / Chem-D4T / DNA / DNA (> 10) / Gag-Pol polyprotein
Similarity search - Component
Biological speciesHuman immunodeficiency virus type 1 group M subtype B
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.53 Å
AuthorsBertoletti, N. / Anderson, K.S.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM049551 United States
CitationJournal: Molecules / Year: 2020
Title: Post-Catalytic Complexes with Emtricitabine or Stavudine and HIV-1 Reverse Transcriptase Reveal New Mechanistic Insights for Nucleotide Incorporation and Drug Resistance.
Authors: Bertoletti, N. / Chan, A.H. / Schinazi, R.F. / Anderson, K.S.
History
DepositionApr 27, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 4, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.2Oct 16, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
P: DNA Primer 21-mer
T: DNA template 27-mer
A: Reverse transcriptase/ribonuclease H
B: p51 RT
hetero molecules


Theoretical massNumber of molelcules
Total (without water)133,58814
Polymers132,2704
Non-polymers1,31810
Water2,504139
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area16210 Å2
ΔGint-155 kcal/mol
Surface area44340 Å2
MethodPISA
Unit cell
Length a, b, c (Å)165.569, 170.468, 103.343
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221
Space group name HallC2c2
Symmetry operation#1: x,y,z
#2: x,-y,-z
#3: -x,y,-z+1/2
#4: -x,-y,z+1/2
#5: x+1/2,y+1/2,z
#6: x+1/2,-y+1/2,-z
#7: -x+1/2,y+1/2,-z+1/2
#8: -x+1/2,-y+1/2,z+1/2
Components on special symmetry positions
IDModelComponents
11A-784-

HOH

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Components

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DNA chain , 2 types, 2 molecules PT

#1: DNA chain DNA Primer 21-mer


Mass: 6476.240 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#2: DNA chain DNA template 27-mer


Mass: 8392.398 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Protein , 2 types, 2 molecules AB

#3: Protein Reverse transcriptase/ribonuclease H / Pr160Gag-Pol


Mass: 64653.090 Da / Num. of mol.: 1 / Mutation: C280S, Q258C
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)
Strain: isolate HXB2 / Gene: gag-pol / Production host: Escherichia coli BL21 (bacteria)
References: UniProt: P04585, RNA-directed DNA polymerase, DNA-directed DNA polymerase, retroviral ribonuclease H
#4: Protein p51 RT


Mass: 52748.418 Da / Num. of mol.: 1 / Mutation: C280S
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)
Strain: isolate HXB2 / Gene: gag-pol / Production host: Escherichia coli BL21 (bacteria)
References: UniProt: P04585, Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases, Hydrolases; Acting on ester bonds

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Non-polymers , 6 types, 149 molecules

#5: Chemical ChemComp-D4M / [(5R)-5-(5-METHYL-2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)-2,5-DIHYDROFURAN-2-YL]METHYL DIHYDROGEN PHOSPHATE / 2',3'-DIDEOXY-2',3-DIDEHYDROTHYMIDINE 5'-MONOPHOSPHATE / 2',3'-DEHYDRO-2',3'-DEOXY-THYMIDINE 5'-MONOPHOSPHATE


Type: DNA linking / Mass: 304.193 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H13N2O7P / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: SO4
#7: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Mg
#8: Chemical ChemComp-D4T / 2',3'-DEHYDRO-2',3'-DEOXY-THYMIDINE 5'-TRIPHOSPHATE


Mass: 464.153 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H15N2O13P3 / Feature type: SUBJECT OF INVESTIGATION
#9: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#10: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 139 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.74 Å3/Da / Density % sol: 55.16 % / Description: thin plate
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6
Details: 6-10% (w/v) PEG 8000, 15 mM magnesium sulfate, and 50 mM MES adjusted at pH 6.0

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.9792 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Apr 16, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 2.53→50 Å / Num. obs: 47964 / % possible obs: 97.9 % / Redundancy: 7.23 % / Biso Wilson estimate: 46.19 Å2 / CC1/2: 0.997 / CC star: 0.997 / Rsym value: 0.147 / Net I/σ(I): 13.44
Reflection shellResolution: 2.53→2.68 Å / Redundancy: 6.78 % / Mean I/σ(I) obs: 2.13 / Num. unique obs: 6782 / CC1/2: 0.768 / CC star: 0.768 / Rsym value: 0.989 / % possible all: 87.1

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Processing

Software
NameVersionClassification
PHENIX1.14_3260refinement
XDSdata reduction
XDSdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6OR7

6or7


Resolution: 2.53→47.68 Å / SU ML: 0.3688 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 24.4341
RfactorNum. reflection% reflection
Rfree0.2312 2398 5 %
Rwork0.182 --
obs0.1844 47959 97.92 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 54.28 Å2
Refinement stepCycle: LAST / Resolution: 2.53→47.68 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7541 845 76 139 8601
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00688762
X-RAY DIFFRACTIONf_angle_d0.907312117
X-RAY DIFFRACTIONf_chiral_restr0.04931336
X-RAY DIFFRACTIONf_plane_restr0.00531378
X-RAY DIFFRACTIONf_dihedral_angle_d18.16735057
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.53-2.580.358930.30591756X-RAY DIFFRACTION64.7
2.58-2.640.35611410.28842683X-RAY DIFFRACTION99.89
2.64-2.70.31961430.2672705X-RAY DIFFRACTION100
2.7-2.770.38891430.25382726X-RAY DIFFRACTION100
2.77-2.840.29021410.24162688X-RAY DIFFRACTION100
2.84-2.930.30921430.23952704X-RAY DIFFRACTION100
2.93-3.020.3111440.22222730X-RAY DIFFRACTION100
3.02-3.130.27081430.22892725X-RAY DIFFRACTION100
3.13-3.250.24311420.21462693X-RAY DIFFRACTION100
3.25-3.40.28251440.19312743X-RAY DIFFRACTION100
3.4-3.580.23421430.17752720X-RAY DIFFRACTION100
3.58-3.810.21481440.17092728X-RAY DIFFRACTION100
3.81-4.10.19761440.15122747X-RAY DIFFRACTION99.97
4.1-4.510.18691450.14172754X-RAY DIFFRACTION100
4.51-5.160.17251460.1382766X-RAY DIFFRACTION99.97
5.16-6.50.20871470.1642791X-RAY DIFFRACTION100
6.5-47.680.18481520.15672902X-RAY DIFFRACTION99.9
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.057076351470.01205657569490.1727883027363.14108547318-0.2403222633222.96304403821-0.03117939196290.632286239707-0.322632535575-0.9969186216060.1125710143110.4472274687880.75554120031-0.303970420594-0.07452604292320.710142696321-0.156828986918-0.1551039290420.501583401473-0.04200294336170.52630603446431.6335381333-9.88370433255-28.9694052834
24.83439497223-0.570871325303-0.3355398613176.105478173870.5983449345233.100323136490.2772793810380.615450036560.0802348443227-1.26798999798-0.00798892499868-0.447834344070.502420843002-0.051581271397-0.2760776649880.799563279532-0.109144319512-0.06681093255630.472189075283-0.06113310380710.40058000341540.907769123-2.70381974743-33.1528998374
31.848642586640.02917346168120.2342314362912.90723811248-0.391805470450.7525292880250.0358095532831-0.0153872627884-0.0670181302462-0.2118314890620.02196964496340.3418948184240.0419972151341-0.194517748621-0.05182817049690.239925719639-0.0319926367551-0.07679069210720.2930505912580.004372145566670.19163336453238.21506182425.62469804981-19.3271122889
45.20306030849-3.17321445806-3.302447875998.050773804484.512193537384.7292742949-0.01296925584310.254345811951-0.132687387372-1.31675159448-0.08656451429890.2995482236680.4430602527630.01466186734550.00581123220661.04160710224-0.154810084822-0.2372686086470.5169530279880.0347114919270.50654044778330.4827309239-14.928345629-33.9132537751
50.6400879661810.0226230876641-0.4738110440733.51246330755-0.8549086280650.97841972899-0.01700205277680.06900337110110.0501993884207-0.4883074513630.0495690586111-0.2273331334860.1903821992740.0281995241731-0.0438517845820.2823660854850.007110339775450.009465452640530.311013429643-0.02427335834770.25866641520448.4161722539.13004196193-21.1816567249
61.11139229705-0.280809892849-0.1710118781414.30645686882-0.6850589132290.2378532964070.03041883006590.258595887130.15598389472-0.753109848827-0.109010821299-0.5183644262880.06886033895670.083007836307-0.02141421138730.350723038728-0.03245764929990.08228077248770.3508811363310.05273897089810.35576522373247.353856556532.1669890963-30.8757684103
71.18914375337-1.217601769161.090024203952.3967622827-0.8055566435351.26895287469-0.109546885669-0.07106009404680.3245913140080.236425187556-0.103082116147-0.638131477305-0.0411751403535-0.06722690437150.198403168990.272474636835-0.0720814447550.06327255426330.3179855256360.04338755716890.44456839114737.372220306253.1454643356-28.5740447585
81.67440573831-0.1208979881890.2338958312723.22318988263-0.2212337585582.406228773440.05265210185890.1334459754390.0819782344502-0.134764332831-0.0515890508189-0.008809664365650.093250848906-0.09496295843050.01056741543560.218676616621-0.02467304966430.06059702022980.2682799337270.05301665495240.28088321535724.103884136858.1105530013-43.8792765393
92.86528027001-1.39789433622-0.3985739410053.713562123731.737943111910.994918751371-0.1793265589870.384469688147-0.9720682461060.0300929899498-0.5618139559591.281027771460.436387795189-0.07098206964230.5552352743190.437386556513-0.04290508741120.09578855671750.6686942268570.1345175031251.047819078648.5745441474915.7011219449-9.3423180188
103.43350345412-1.693635796261.099482539635.40263230570.450017607472.44922690423-0.142821697831-0.141237291935-0.04655735866750.7914149793390.3003524827380.2466146013350.02997598719160.0609848865405-0.109749107880.2954955230830.04778174048920.05691081101640.3533819497520.04963788624440.25747357274628.907535171821.6078860336-6.66235531248
111.67371676642.77965784715-0.9982641968026.20722814142-4.07981379274.302684017960.121996439870.4358439074830.505426766383-0.233756556552-0.7696393069360.687257107181-0.561422907432-0.8726604116290.4572828611930.2739122082260.0792819859678-0.04925907836120.8121918128860.05636538737950.8606522837929.8724846316321.9609908464-12.2307645456
121.35452657454-1.17593605121-0.6868231985551.338346130480.9043563174811.08707731958-0.0679016587394-0.28493276933-0.2557419290250.5381424008350.1218614315990.206564299222-0.204266841416-0.3146562622470.01032920476780.5236947438350.08102939250520.1197392707940.5180608458910.1708735058280.41528083652621.621762771822.523576613-2.72970705868
133.127778270821.159285175290.5892755491473.910134855271.498920800413.137023861180.0638793176658-0.746125832306-0.03009088049730.784923412455-0.4298274397060.388699806082-0.374302845411-0.2642738462050.3546853400360.7769224924770.0629712261490.2757874713480.6899577767160.145305746090.5119790725812.298499553734.538483160410.1200955852
145.21744358381.98204233468-0.4625857542454.98954020919-4.226168738118.063822560960.443143834212-0.767193673360.6905996936780.8149945042280.8542551487540.659501787146-1.519787876090.466634990679-0.9457579995490.9307361706130.05393958325020.1796939270360.6631589022790.1047830702510.39069350534617.098705226339.65816043679.6769757612
152.339979711462.16169330949-1.178061845426.33982715281-2.40993653112.055429908170.212309073061-0.3726333283890.057883724570.439146055545-0.1475350399420.482689092362-0.147953290942-0.0738082902476-0.1270648701740.2884989231640.0001492492979750.08173014641050.41610644423-0.01459078560880.38484970865410.511325793865.4441105553-23.3268619415
166.25704444396-1.44978166429-0.05033593631363.84202823229-1.116460456942.33351085152-0.117065094373-0.06807950430610.1532378759860.3671483739580.120432362553-0.112596486783-0.01820566304270.0246644838484-0.01495117210550.2726183751270.0144183540260.02603853451980.272715800731-0.02983342705810.22563585848521.895456240144.4136232526-13.8902736821
174.101216802450.917204933763-4.942948399193.84571155314-1.205420532956.0954717321-1.158425385711.861230059080.113041351103-0.3940270178630.6350075549850.9116751936120.240114401366-1.434157381910.5045170328510.454041020416-0.09954092910610.07832182186730.59577420518-0.05892156529880.9464893323359.2278356095340.4653183585-42.0954782205
180.127402336445-0.7511280976190.6422607540974.95062470678-4.171802238983.695033447150.0472775142027-0.0282642850530.1647364506140.3913411661260.7382104787440.200747625657-0.231607385488-0.0706543795285-0.7145543525490.5376812854530.132961221357-0.006677978307970.527412326639-0.02449800383060.58350020486822.955942891237.9860726868-35.7028634573
198.497290762722.541970717120.7369149691974.30178238346-2.075956209215.888508847320.31340209415-0.127609430848-1.498204485481.11069153381-0.120573262923-0.5658374944991.39612780338-0.696938585307-0.3380700668351.047605246380.219700770843-0.06647170125430.4701537575330.1006350058990.78663214317726.638843254122.0760765808-33.7697362867
206.67242268386-2.94571688565-0.1699584876567.81343917998-2.987849856566.649547236250.8661050847610.171213819672-0.154960810462-1.635944860030.07301711685410.397782430946-0.136124825114-0.300376052983-0.8302144741620.6384270585260.07881533870450.01975847962430.3272925466420.01262915601240.35579503686343.331525064714.6112832996-34.2054250425
214.275433263295.609734981263.807072813388.13245916714.676010978613.48471342213-0.9054980525480.911966141196-0.650174402585-2.474147920560.612997980712-0.5808323551410.0739414330532-0.122347169199-0.1084317110780.846818822138-0.146356658947-0.02717591611060.5301510952130.03357207777580.53539538163935.4987531984.54482562839-33.761571472
223.488684092612.37722958921-0.8763048028041.60928323472-0.6757535847970.324634030164-0.105109350358-0.02028513865420.381989142291-0.580800047370.01876734578870.5698178921330.26829988191-0.1658739745810.2105215664750.6075043866370.0129508806826-0.1530922537190.3479037907220.01895808086720.32421653833737.79057365519.4382093612-28.6073630367
239.86755328559-4.631082147042.22949487458.418230119131.140487321542.933057321820.3034368150020.417445345533-0.9238980606490.329597597195-0.3638651720411.497409423180.825708314298-0.341055866088-0.0329760967530.7268042604780.0531093072412-0.05452570461360.4449699461250.1669667673730.5162519899521.401451166932.3938512497-36.5643512052
244.501121554380.2507828566485.722559110227.22274799535-2.085553875628.36390919938-1.669138867860.2348448309110.8701475129790.2334731996620.633377076901-1.36944084233-1.66258549438-0.3104800579360.9055154911480.5332464628680.15187403132-0.02045175203530.825603106877-0.00408573270480.9925839332015.8042037424950.1321808566-42.6052485882
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 1:62)
2X-RAY DIFFRACTION2(chain A and resid 63:79)
3X-RAY DIFFRACTION3(chain A and resid 80:125)
4X-RAY DIFFRACTION4(chain A and resid 126:142)
5X-RAY DIFFRACTION5(chain A and resid 143:277)
6X-RAY DIFFRACTION6(chain A and resid 278:387)
7X-RAY DIFFRACTION7(chain A and resid 388:448)
8X-RAY DIFFRACTION8(chain A and resid 449:555)
9X-RAY DIFFRACTION9(chain B and resid 6:16)
10X-RAY DIFFRACTION10(chain B and resid 17:82)
11X-RAY DIFFRACTION11(chain B and resid 83:86)
12X-RAY DIFFRACTION12(chain B and resid 96:153)
13X-RAY DIFFRACTION13(chain B and resid 154:204)
14X-RAY DIFFRACTION14(chain B and resid 205:240)
15X-RAY DIFFRACTION15(chain B and resid 241:324)
16X-RAY DIFFRACTION16(chain B and resid 325:429)
17X-RAY DIFFRACTION17(chain P and resid 805:808)
18X-RAY DIFFRACTION18(chain P and resid 809:812)
19X-RAY DIFFRACTION19(chain P and resid 813:816)
20X-RAY DIFFRACTION20(chain P and resid 818:821)
21X-RAY DIFFRACTION21(chain T and resid 704:707)
22X-RAY DIFFRACTION22(chain T and resid 708:711)
23X-RAY DIFFRACTION23(chain T and resid 712:721)
24X-RAY DIFFRACTION24(chain T and resid 722:725)

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