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- PDB-6wph: Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsD... -

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Basic information

Entry
Database: PDB / ID: 6wph
TitleStructure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and (-)-FTC
Components
  • DNA Primer 21-mer
  • DNA template 27-mer
  • Reverse transcriptase/ribonuclease H
  • p51 RT
KeywordsTRANSFERASE/DNA / Reverse Transcriptase / ternary complex / (-)FTC / chain terminator / Emtricitabine / stereochemistry / TRANSFERASE / TRANSFERASE-DNA complex
Function / homology
Function and homology information


integrase activity / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / Minus-strand DNA synthesis / Plus-strand DNA synthesis / Uncoating of the HIV Virion / 2-LTR circle formation / Vpr-mediated nuclear import of PICs / Early Phase of HIV Life Cycle / Integration of provirus ...integrase activity / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / Minus-strand DNA synthesis / Plus-strand DNA synthesis / Uncoating of the HIV Virion / 2-LTR circle formation / Vpr-mediated nuclear import of PICs / Early Phase of HIV Life Cycle / Integration of provirus / APOBEC3G mediated resistance to HIV-1 infection / Binding and entry of HIV virion / viral life cycle / HIV-1 retropepsin / symbiont-mediated activation of host apoptosis / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / Assembly Of The HIV Virion / Budding and maturation of HIV virion / protein processing / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / RNA stem-loop binding / host multivesicular body / viral penetration into host nucleus / RNA-directed DNA polymerase activity / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / peptidase activity / host cell / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / aspartic-type endopeptidase activity / Hydrolases; Acting on ester bonds / DNA-directed DNA polymerase activity / symbiont-mediated suppression of host gene expression / viral translational frameshifting / lipid binding / symbiont entry into host cell / host cell nucleus / host cell plasma membrane / virion membrane / structural molecule activity / DNA binding / zinc ion binding / identical protein binding / membrane
Similarity search - Function
Reverse transcriptase connection / Reverse transcriptase connection domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral / Integrase, N-terminal zinc-binding domain ...Reverse transcriptase connection / Reverse transcriptase connection domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral / Integrase, N-terminal zinc-binding domain / Integrase, C-terminal, retroviral / Integrase DNA binding domain profile. / Immunodeficiency lentiviral matrix, N-terminal / gag gene protein p17 (matrix protein) / RNase H / Integrase core domain / Integrase, catalytic core / Integrase catalytic domain profile. / Retropepsin-like catalytic domain / Matrix protein, lentiviral and alpha-retroviral, N-terminal / Retroviral nucleocapsid Gag protein p24, C-terminal domain / Gag protein p24 C-terminal domain / RNase H type-1 domain profile. / Ribonuclease H domain / Retropepsins / Retroviral aspartyl protease / Aspartyl protease, retroviral-type family profile. / Peptidase A2A, retrovirus, catalytic / Retrovirus capsid, C-terminal / Reverse transcriptase domain / Reverse transcriptase (RNA-dependent DNA polymerase) / Reverse transcriptase (RT) catalytic domain profile. / Retroviral matrix protein / Retrovirus capsid, N-terminal / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Aspartic peptidase, active site / Eukaryotic and viral aspartyl proteases active site. / Aspartic peptidase domain superfamily / Ribonuclease H superfamily / Ribonuclease H-like superfamily / Reverse transcriptase/Diguanylate cyclase domain / DNA/RNA polymerase superfamily
Similarity search - Domain/homology
Chem-43X / 2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE / DNA / DNA (> 10) / Gag-Pol polyprotein
Similarity search - Component
Biological speciesHuman immunodeficiency virus type 1 group M subtype B
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.72 Å
AuthorsBertoletti, N. / Anderson, K.S.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM049551 United States
CitationJournal: Molecules / Year: 2020
Title: Post-Catalytic Complexes with Emtricitabine or Stavudine and HIV-1 Reverse Transcriptase Reveal New Mechanistic Insights for Nucleotide Incorporation and Drug Resistance.
Authors: Bertoletti, N. / Chan, A.H. / Schinazi, R.F. / Anderson, K.S.
History
DepositionApr 27, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 4, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 2.0May 29, 2024Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Derived calculations / Non-polymer description / Structure summary
Category: atom_site / atom_site_anisotrop ...atom_site / atom_site_anisotrop / chem_comp / chem_comp_atom / chem_comp_bond / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_unobs_or_zero_occ_atoms / struct_site
Item: _atom_site.auth_comp_id / _atom_site.label_comp_id ..._atom_site.auth_comp_id / _atom_site.label_comp_id / _atom_site_anisotrop.pdbx_auth_comp_id / _atom_site_anisotrop.pdbx_label_comp_id / _chem_comp.formula / _chem_comp.formula_weight / _chem_comp.id / _chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _pdbx_entity_nonpoly.comp_id / _pdbx_nonpoly_scheme.mon_id / _pdbx_nonpoly_scheme.pdb_mon_id / _pdbx_unobs_or_zero_occ_atoms.auth_comp_id / _pdbx_unobs_or_zero_occ_atoms.label_comp_id / _struct_site.details / _struct_site.pdbx_auth_comp_id
Revision 2.1Oct 23, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Reverse transcriptase/ribonuclease H
B: p51 RT
P: DNA Primer 21-mer
T: DNA template 27-mer
hetero molecules


Theoretical massNumber of molelcules
Total (without water)133,07010
Polymers132,1554
Non-polymers9156
Water39622
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area13970 Å2
ΔGint-98 kcal/mol
Surface area44140 Å2
MethodPISA
Unit cell
Length a, b, c (Å)165.980, 168.054, 101.916
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221
Space group name HallC2c2
Symmetry operation#1: x,y,z
#2: x,-y,-z
#3: -x,y,-z+1/2
#4: -x,-y,z+1/2
#5: x+1/2,y+1/2,z
#6: x+1/2,-y+1/2,-z
#7: -x+1/2,y+1/2,-z+1/2
#8: -x+1/2,-y+1/2,z+1/2
Components on special symmetry positions
IDModelComponents
11B-501-

MG

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Components

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Protein , 2 types, 2 molecules AB

#1: Protein Reverse transcriptase/ribonuclease H


Mass: 64521.895 Da / Num. of mol.: 1 / Mutation: C280S, Q258C
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)
Strain: isolate HXB2 / Gene: gag-pol / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: P04585, RNA-directed DNA polymerase, DNA-directed DNA polymerase, retroviral ribonuclease H
#2: Protein p51 RT


Mass: 52748.418 Da / Num. of mol.: 1 / Mutation: C280S
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)
Strain: isolate HXB2 / Gene: gag-pol / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: P04585, Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases, Hydrolases; Acting on ester bonds

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DNA chain , 2 types, 2 molecules PT

#3: DNA chain DNA Primer 21-mer


Mass: 6476.240 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#4: DNA chain DNA template 27-mer


Mass: 8408.397 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Non-polymers , 5 types, 28 molecules

#5: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#6: Chemical ChemComp-43X / [(2R,5S)-5-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)-1,3-oxathiolan-2-yl]methyl dihydrogen phosphate / FTC-MP


Mass: 327.227 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H11FN3O6PS / Feature type: SUBJECT OF INVESTIGATION
#7: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#8: Chemical ChemComp-DGP / 2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE


Mass: 347.221 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H14N5O7P
#9: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 22 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.67 Å3/Da / Density % sol: 53.99 % / Description: thin plate
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6
Details: 6-10% (w/v) PEG 8000, 15 mM magnesium sulfate, and 50 mM MES adjusted at pH 6.0

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: NSLS-II / Beamline: 17-ID-2 / Wavelength: 0.9793 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Sep 16, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9793 Å / Relative weight: 1
ReflectionResolution: 2.71→30 Å / Num. obs: 38359 / % possible obs: 98.4 % / Redundancy: 25.3 % / Biso Wilson estimate: 64.91 Å2 / CC1/2: 0.999 / CC star: 0.999 / Rsym value: 0.161 / Net I/σ(I): 20.33
Reflection shellResolution: 2.71→2.88 Å / Redundancy: 24.1 % / Mean I/σ(I) obs: 2.68 / Num. unique obs: 5620 / CC1/2: 0.911 / CC star: 0.911 / Rsym value: 0.977 / % possible all: 90.9

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Processing

Software
NameVersionClassification
XDSdata scaling
PHENIX1.14_3260refinement
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6OR7

6or7


Resolution: 2.72→29.52 Å / SU ML: 0.4372 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 29.306
RfactorNum. reflection% reflection
Rfree0.2566 1885 5 %
Rwork0.1969 --
obs0.1999 37715 98.11 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 65.1 Å2
Refinement stepCycle: LAST / Resolution: 2.72→29.52 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7480 824 53 22 8379
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00758677
X-RAY DIFFRACTIONf_angle_d0.941512000
X-RAY DIFFRACTIONf_chiral_restr0.05241328
X-RAY DIFFRACTIONf_plane_restr0.00631388
X-RAY DIFFRACTIONf_dihedral_angle_d17.14195020
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.72-2.80.48921120.45242119X-RAY DIFFRACTION75.78
2.8-2.880.38451460.30782778X-RAY DIFFRACTION99.9
2.88-2.970.32691450.25692755X-RAY DIFFRACTION99.93
2.97-3.080.34191460.24732781X-RAY DIFFRACTION99.93
3.08-3.20.31191450.24522782X-RAY DIFFRACTION99.93
3.2-3.350.31631460.24572775X-RAY DIFFRACTION99.97
3.35-3.520.32831470.2282796X-RAY DIFFRACTION100
3.52-3.740.2631470.19592786X-RAY DIFFRACTION100
3.74-4.030.23781470.17682801X-RAY DIFFRACTION100
4.03-4.440.20881490.15822831X-RAY DIFFRACTION100
4.44-5.080.20711480.15082813X-RAY DIFFRACTION100
5.08-6.390.25221510.18232853X-RAY DIFFRACTION100
6.39-29.520.20771560.17862960X-RAY DIFFRACTION99.84
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.9005016414990.0125451487501-0.2030016755381.54455325513-0.2925915047160.96808398051-0.2013623564110.255472274779-0.422293633161-0.2963963034820.05240493216750.2723600054250.804947465255-0.252932952212-0.0114110212110.678585845938-0.0837146744956-0.136589757180.513969566359-0.09622763259780.68244286920731.3264283212-9.61973140739-27.7638670602
21.71389495842-0.3300464872670.07195775588890.392606806710.05194185793690.295547392752-0.3448929309781.070338754160.704699683401-0.9228397291220.337541341088-0.461104635266-0.0976512999678-0.2394114776910.02324883900680.966933590698-0.156707704895-0.167316565590.640871638357-0.2241603894650.88174311373238.984046702-2.63514048886-33.5429261493
30.903229238048-0.5788016950890.1392880983620.4650659336290.03802164094520.226169515322-0.09145156958480.124326141085-0.243097241154-0.1174607595970.065162176575-0.04716824959010.0956594366368-0.00539783551782-7.26943561153E-50.467236253298-0.0502267371488-0.01579561442280.412218438048-0.02346127434120.43442311402838.23955655655.6429989611-18.8270568726
40.0688000824988-0.08283426931690.07619052958080.101522448553-0.1026695819350.134225998169-0.3552009049240.201853985577-0.00605135779025-0.4407000410690.0723276327559-0.03693383564120.4030270330690.0395802670860.001232722773540.982135587952-0.197983183272-0.1152050906180.720267264906-0.1198975982780.69280346262228.2765424172-11.8223909591-30.3750705865
50.248986237402-0.03611997817180.02147575530361.75020176586-0.5417467481540.6251745954210.012201952198-0.0430745185624-0.109407784753-0.1587661579530.0139193410322-0.2945580942970.2687282888120.079429714178-0.00052032553420.3769693475720.0281836220764-0.006308355533820.311445178339-0.04234410254490.3105809340748.3019261038.99899814538-21.1542729253
60.2055368434240.1773198280670.1965486712581.7577606031-0.2698628063540.301234706687-0.03617034177540.06331366220.225751730549-0.219537319253-0.13068677391-0.427450407505-0.111082948267-0.0496654158515-0.001501086338040.352930283356-0.0001140145927650.01735381304370.4171859901630.03965716494290.37530378740546.966761621331.8106242449-30.7895196382
70.286277503965-0.09010973356980.1052128358661.30127994565-0.2569680413480.849137866604-0.016902708812-0.008083829158690.1577687611240.102506897569-0.136489454482-0.286684895594-0.2088406939680.0723039215139-6.50101734954E-50.36214667163-0.02950365481270.06462030229660.38542381617-0.01233135720510.45555036120137.22298737252.6688287504-28.3494920455
81.10333605020.14844384929-0.2229079730440.6486076437710.0524323164812.137323706130.1982646717680.1321614976020.236878120641-0.049402080391-0.127983323771-0.005182295390410.0121189551815-0.0417908442758-0.0001132011417240.3478451497850.01011148793660.08576876668080.2943832394160.07096809622640.39280396901123.859007967657.3553175622-43.6344087308
90.102680245179-0.120480920576-0.05445576006330.1336636629840.06546889759440.0822999100776-0.1377490690310.31248475286-0.2458408082310.3756585114970.1255410004911.272603961060.256964818076-0.57757716052-0.0006744444439670.766231298812-0.02345163289590.1626622485090.744155524116-0.02611558231271.001299029328.8324188208815.2885115187-8.61162747087
101.05081566904-0.9912708060070.1985779226561.071267394660.1112160644790.535126679226-0.0287506101235-0.0505768429210.02439649881990.2105224102570.04725118405370.0963568943260.02182286620030.0537502886256-0.000114892730410.4502410251190.05389014197660.01714244832620.5263121116370.01013158721640.35830460129128.980852334120.9596682848-6.19954573768
110.0986285332436-0.0186405805063-0.05919533247840.07712989860730.01250364244830.034865084298-0.180262369238-0.15924752101-0.101090203831-0.4116636371910.1183423562240.161788316366-0.0745117986864-0.0217142774517-0.0004389639147350.3287009466320.0386398917157-0.04485582150750.7251902952210.02017656466510.67412937026710.014822767721.6053378607-11.6801194081
121.824524843080.074870630754-0.2106755205410.2497861712540.2691665334150.3725553704980.154222980874-0.193866163186-0.6042331381820.355747822391-0.0223244759395-0.0208081789632-7.93535814509E-5-0.07417542562820.689124229930.471345257770.06742653720340.1593037030080.4539230399040.1665925994020.16408881060321.787644958222.1354990288-2.23614288944
130.975154006269-0.4401291492970.5768078732760.9665127371610.04171905878840.5145708268620.0519283088829-0.442191276944-0.1552842933370.266317701581-0.1309727386040.225556109891-0.3292999237790.304716914388-0.002172561397490.64430913527-0.122138367030.2071440650550.6176050135180.006587210216520.28060722755712.367846730634.387077652510.5046105325
140.5314074725250.133913132137-0.382711483060.2119219281470.1287575017140.5620623089080.622650377398-0.933773187547-0.02165914098110.194396243710.1768689538840.412490102816-0.9430199063460.5915585209130.0685469836551.12273236848-0.2640866660820.03520591094321.045794320740.2287362587450.55667852821317.10752153839.74767181489.93055857869
151.605037945480.524743448496-0.7962914892930.830759812402-0.1743092653811.30876021820.169080204383-0.1560918793630.2729812504940.126676741185-0.06540024732950.280432268552-0.272700179734-0.268219448191-0.0001090755229220.3625607099780.004209065864120.1043626324720.489005146099-0.006808358307650.52867485355910.367642585364.73470165-23.141922047
162.292835299220.428744948422-0.7436587758821.86657235463-0.2812963010311.1328472773-0.02974008223970.1496997129990.233077295895-0.04364576208680.0933449257158-0.1590557981-0.00930883659725-0.0435576787028-8.83911994096E-50.3931877593340.01459225984470.02785557987270.449396119336-0.03331669323060.33598347350422.013903037343.9706878761-13.5243125497
170.01074121735110.0322064985564-0.01552977625440.118449537829-0.02059208623620.0600941205454-1.021165090630.512946936550.301447875081-0.3224317159440.169298849250.716824657730.0464979212041-0.7665634391830.004703482688880.482921146373-0.05265185243370.0563708120980.674465596679-0.02238775829261.113458079169.0543551817739.9438687592-41.5432896237
180.02905707448790.06046803638010.01654746362860.1658816567550.007428965924410.0421525579474-0.129446519008-0.201918904678-0.622377351891-0.5059895612060.6339654358460.5390954551040.5671006895250.06905938990410.001829428744330.5854972987680.137964620773-0.09353909653330.5985723536470.07803035280860.69295973190722.772232801837.6787057774-35.2926095962
190.07506470225670.003335309523810.03123732500790.04797884028380.03686945503030.0379774827653-0.224289104129-0.0571079047767-0.6673048850120.3667561741310.218572556716-0.578696815934-0.103347548626-0.378875868111-0.001822692529120.9314819732390.1118926738190.1576069552420.640689759178-0.05893999455541.0430017196326.289194344922.041508755-33.1042562964
200.0190364800373-0.02653428303260.01854431097290.0325477608434-0.01999605659780.01808720019130.24489565122-0.287657974059-0.123773841694-0.06019377263670.1920278215510.147540301376-0.254288629783-0.4647841000870.002163212103240.7152441007840.06287036533680.02516527926390.554622574880.04542510464440.48861000810642.807411390614.4208024227-33.8016128248
214.46204485792-2.041896403030.8167109786332.37693504750.896730470521.26435412924-0.6625765868820.380909394260.602182750592-1.353575569120.185537441951-1.029357792190.114677573625-0.212548488097-0.3443243597970.941592091706-0.1542823917070.1511798213310.544334901139-0.005461066678290.6126748858434.65617728414.61973063568-33.3598012248
220.01337954644960.0131951577753-0.003795703666840.063356253882-0.01827304460790.0369688907580.02802950075950.06598093768510.294478402558-0.243295573308-0.07459807127110.002488773972670.422206624090.1332199763290.0003833869793980.615723933921-0.00691441725011-0.2152837496910.4947052059150.02851477866980.4815986231337.469611574819.3085812382-28.1617998312
230.1507222619050.1209105417590.1910909762820.4919090930570.4625905202880.4812328517890.07037072962060.677215595835-0.3701619895980.624625603038-0.4648992721560.7539564466870.0766654344973-0.0265970947447-0.004641929706780.7847212100630.15425251235-0.01409225918950.7820539903940.02159464575760.73994880575820.976745217332.3071454088-36.0012854045
240.1103744989810.06470707544340.008561297542280.0953895931117-0.02385484391180.0449746434706-1.02825302566-0.0703655764201-0.4242059431860.5251816214060.749027450324-0.572455015143-0.632969867948-0.6255327827240.001272420849140.612240092060.0370433578090.03927977973150.7300427912870.07832107910161.038984528725.6053336616849.6080769724-42.3121771512
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 1:62)
2X-RAY DIFFRACTION2(chain A and resid 63:79)
3X-RAY DIFFRACTION3(chain A and resid 80:125)
4X-RAY DIFFRACTION4(chain A and resid 126:142)
5X-RAY DIFFRACTION5(chain A and resid 143:277)
6X-RAY DIFFRACTION6(chain A and resid 278:387)
7X-RAY DIFFRACTION7(chain A and resid 388:448)
8X-RAY DIFFRACTION8(chain A and resid 449:555)
9X-RAY DIFFRACTION9(chain B and resid 6:16)
10X-RAY DIFFRACTION10(chain B and resid 17:82)
11X-RAY DIFFRACTION11(chain B and resid 83:86)
12X-RAY DIFFRACTION12(chain B and resid 96:153)
13X-RAY DIFFRACTION13(chain B and resid 154:204)
14X-RAY DIFFRACTION14(chain B and resid 205:240)
15X-RAY DIFFRACTION15(chain B and resid 241:324)
16X-RAY DIFFRACTION16(chain B and resid 325:428)
17X-RAY DIFFRACTION17(chain P and resid 805:808)
18X-RAY DIFFRACTION18(chain P and resid 809:812)
19X-RAY DIFFRACTION19(chain P and resid 813:816)
20X-RAY DIFFRACTION20(chain P and resid 818:821)
21X-RAY DIFFRACTION21(chain T and resid 704:707)
22X-RAY DIFFRACTION22(chain T and resid 708:711)
23X-RAY DIFFRACTION23(chain T and resid 712:721)
24X-RAY DIFFRACTION24(chain T and resid 722:724)

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