+Open data
-Basic information
Entry | Database: PDB / ID: 6w6w | |||||||||||||||||||||
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Title | Cryo-EM structure of CST bound to telomeric single-stranded DNA | |||||||||||||||||||||
Components |
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Keywords | STRUCTURAL PROTEIN/DNA / Telomere homeostasis / telomere packaging / telomerase terminator / DNA replication / Double-stranded breaks repair / single-stranded DNA-binding proteins / higher-order protein assembly / DNA-induced oligomeriization / STRUCTURAL PROTEIN / STRUCTURAL PROTEIN-DNA complex | |||||||||||||||||||||
Function / homology | Function and homology information CST complex / telomerase inhibitor activity / telomere maintenance via telomere lengthening / Telomere C-strand synthesis initiation / single-stranded telomeric DNA binding / Polymerase switching on the C-strand of the telomere / G-rich strand telomeric DNA binding / telomere capping / bone marrow development / intermediate filament cytoskeleton ...CST complex / telomerase inhibitor activity / telomere maintenance via telomere lengthening / Telomere C-strand synthesis initiation / single-stranded telomeric DNA binding / Polymerase switching on the C-strand of the telomere / G-rich strand telomeric DNA binding / telomere capping / bone marrow development / intermediate filament cytoskeleton / hematopoietic stem cell proliferation / telomeric DNA binding / negative regulation of telomere maintenance via telomerase / replicative senescence / spleen development / regulation of G2/M transition of mitotic cell cycle / telomere maintenance / thymus development / positive regulation of DNA replication / multicellular organism growth / fibrillar center / positive regulation of fibroblast proliferation / single-stranded DNA binding / chromosome, telomeric region / intracellular membrane-bounded organelle / DNA damage response / nucleoplasm / nucleus / cytosol Similarity search - Function | |||||||||||||||||||||
Biological species | Homo sapiens (human) | |||||||||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3 Å | |||||||||||||||||||||
Authors | Lim, C. / Barbour, A.T. / Zaug, A.J. / Goodrich, K.J. / McKay, A.E. / Wuttke, D.S. / Cech, T.R. | |||||||||||||||||||||
Funding support | United States, 6items
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Citation | Journal: Science / Year: 2020 Title: The structure of human CST reveals a decameric assembly bound to telomeric DNA. Authors: Ci Ji Lim / Alexandra T Barbour / Arthur J Zaug / Karen J Goodrich / Allison E McKay / Deborah S Wuttke / Thomas R Cech / Abstract: The CTC1-STN1-TEN1 (CST) complex is essential for telomere maintenance and resolution of stalled replication forks genome-wide. Here, we report the 3.0-angstrom cryo-electron microscopy structure of ...The CTC1-STN1-TEN1 (CST) complex is essential for telomere maintenance and resolution of stalled replication forks genome-wide. Here, we report the 3.0-angstrom cryo-electron microscopy structure of human CST bound to telomeric single-stranded DNA (ssDNA), which assembles as a decameric supercomplex. The atomic model of the 134-kilodalton CTC1 subunit, built almost entirely de novo, reveals the overall architecture of CST and the DNA-binding anchor site. The carboxyl-terminal domain of STN1 interacts with CTC1 at two separate docking sites, allowing allosteric mediation of CST decamer assembly. Furthermore, ssDNA appears to staple two monomers to nucleate decamer assembly. CTC1 has stronger structural similarity to Replication Protein A than the expected similarity to yeast Cdc13. The decameric structure suggests that CST can organize ssDNA analogously to the nucleosome's organization of double-stranded DNA. | |||||||||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6w6w.cif.gz | 314.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6w6w.ent.gz | 233.5 KB | Display | PDB format |
PDBx/mmJSON format | 6w6w.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6w6w_validation.pdf.gz | 852.3 KB | Display | wwPDB validaton report |
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Full document | 6w6w_full_validation.pdf.gz | 857.2 KB | Display | |
Data in XML | 6w6w_validation.xml.gz | 47.4 KB | Display | |
Data in CIF | 6w6w_validation.cif.gz | 72.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w6/6w6w ftp://data.pdbj.org/pub/pdb/validation_reports/w6/6w6w | HTTPS FTP |
-Related structure data
Related structure data | 21567MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | |
EM raw data | EMPIAR-10718 (Title: The structure of human CST reveals a decameric assembly bound to telomeric DNA Data size: 14.3 TB Data #1: Unaligned movies of CST with 3xTEL DNA oligomer at 0 degrees stage tilt [micrographs - multiframe] Data #2: Unaligned movies of CST with 3xTEL DNA oligomer at 30 degrees stage tilt [micrographs - multiframe] Data #3: Gain reference for both datasets - gainref_20190729.mrc [micrographs - single frame]) |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 136745.984 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CTC1, C17orf68 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q2NKJ3 #2: DNA chain | | Mass: 1230.854 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) #3: Protein | | Mass: 43001.824 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: STN1, OBFC1 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q9H668 #4: Protein | | Mass: 17285.604 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TEN1, C17orf106 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q86WV5 #5: Chemical | ChemComp-ZN / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Human CST complex / Type: COMPLEX / Entity ID: #1-#4 / Source: RECOMBINANT |
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Molecular weight | Value: 0.2 MDa / Experimental value: YES |
Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Trichoplusia ni (cabbage looper) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 60 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||
Symmetry | Point symmetry: C1 (asymmetric) | ||||||||||||||||||||
3D reconstruction | Resolution: 3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 833627 / Symmetry type: POINT | ||||||||||||||||||||
Atomic model building | Protocol: AB INITIO MODEL | ||||||||||||||||||||
Atomic model building |
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