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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-21563 | |||||||||||||||||||||
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Title | DNA-free CST monomer | |||||||||||||||||||||
![]() | DNA-free CST monomer, C1 refined, sharpened | |||||||||||||||||||||
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Biological species | ![]() | |||||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 6.3 Å | |||||||||||||||||||||
![]() | Lim C / Barbour AT / Zaug AJ / Goodrich KJ / McKay AE / Wuttke DS / Cech TR | |||||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: The structure of human CST reveals a decameric assembly bound to telomeric DNA. Authors: Ci Ji Lim / Alexandra T Barbour / Arthur J Zaug / Karen J Goodrich / Allison E McKay / Deborah S Wuttke / Thomas R Cech / ![]() Abstract: The CTC1-STN1-TEN1 (CST) complex is essential for telomere maintenance and resolution of stalled replication forks genome-wide. Here, we report the 3.0-angstrom cryo-electron microscopy structure of ...The CTC1-STN1-TEN1 (CST) complex is essential for telomere maintenance and resolution of stalled replication forks genome-wide. Here, we report the 3.0-angstrom cryo-electron microscopy structure of human CST bound to telomeric single-stranded DNA (ssDNA), which assembles as a decameric supercomplex. The atomic model of the 134-kilodalton CTC1 subunit, built almost entirely de novo, reveals the overall architecture of CST and the DNA-binding anchor site. The carboxyl-terminal domain of STN1 interacts with CTC1 at two separate docking sites, allowing allosteric mediation of CST decamer assembly. Furthermore, ssDNA appears to staple two monomers to nucleate decamer assembly. CTC1 has stronger structural similarity to Replication Protein A than the expected similarity to yeast Cdc13. The decameric structure suggests that CST can organize ssDNA analogously to the nucleosome's organization of double-stranded DNA. | |||||||||||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 59.8 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.2 KB 14.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 11.9 KB | Display | ![]() |
Images | ![]() | 46.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 78.3 KB | Display | ![]() |
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Full document | ![]() | 77.4 KB | Display | |
Data in XML | ![]() | 494 B | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | DNA-free CST monomer, C1 refined, sharpened | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Heterotrimeric complex of human CST complex without DNA
Entire | Name: Heterotrimeric complex of human CST complex without DNA |
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Components |
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-Supramolecule #1: Heterotrimeric complex of human CST complex without DNA
Supramolecule | Name: Heterotrimeric complex of human CST complex without DNA type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
Molecular weight | Experimental: 200 KDa |
-Macromolecule #1: Homo sapien CTC1
Macromolecule | Name: Homo sapien CTC1 / type: protein_or_peptide / ID: 1 / Details: 2xFLAG-CTCC1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
Sequence | String: MDYKDDDDKD YKDDDDKAAG RAQVPSSEQA WLEDAQVFIQ KTLCPAVKEP NVQLTPLVID CVKTVWLSQG RNQGSTLPLS YSFVSVQDLK THQRLPCCSH LSWSSSAYQA WAQEAGPNGN PLPREQLLLL GTLTDLSADL EQECRNGSLY VRDNTGVLSC ELIDLDLSWL ...String: MDYKDDDDKD YKDDDDKAAG RAQVPSSEQA WLEDAQVFIQ KTLCPAVKEP NVQLTPLVID CVKTVWLSQG RNQGSTLPLS YSFVSVQDLK THQRLPCCSH LSWSSSAYQA WAQEAGPNGN PLPREQLLLL GTLTDLSADL EQECRNGSLY VRDNTGVLSC ELIDLDLSWL GHLFLFPRWS YLPPARWNSS GEGHLELWDA PVPVFPLTIS PGPVTPIPVL YPESASCLLR LRNKLRGVQR NLAGSLVRLS ALVKSKQKAY FILSLGRSHP AVTHVSIIVQ VPAQLVWHRA LRPGTAYVLT ELRVSKIRGQ RQHVWMTSQS SRLLLLKPEC VQELELELEG PLLEADPKPL PMPSNSEDKK DPESLVRYSR LLSYSGAVTG VLNEPAGLYE LDGQLGLCLA YQQFRGLRRV MRPGVCLQLQ DVHLLQSVGG GTRRPVLAPC LRGAVLLQSF SRQKPGAHSS RQAYGASLYE QLVWERQLGL PLYLWATKAL EELACKLCPH VLRHHQFLQH SSPGSPSLGL QLLAPTLDLL APPGSPVRNA HNEILEEPHH CPLQKYTRLQ TPSSFPTLAT LKEEGQRKAW ASFDPKALLP LPEASYLPSC QLNRRLAWSW LCLLPSAFCP AQVLLGVLVA SSHKGCLQLR DQSGSLPCLL LAKHSQPLSD PRLIGCLVRA ERFQLIVERD VRSSFPSWKE LSMPGFIQKQ QARVYVQFFL ADALILPVPR PCLHSATPST PQTDPTGPEG PHLGQSRLFL LCHKEALMKR NFCVPPGASP EVPKPALSFY VLGSWLGGTQ RKEGTGWGLP EPQGNDDNDQ KVHLIFFGSS VRWFEFLHPG QVYRLVAPGP ATPMLFEKDG SSCISRRPLE LAGCASCLTV QDNWTLELES SQDIQDVLDA NKSLPESSLT DLLSDNFTDS LVSFSAEILS RTLCEPLVAS LWMKLGNTGA MRRCVKLTVA LETAECEFPP HLDVYIEDPH LPPSLGLLPG ARVHFSQLEK RVSRSHNVYC CFRSSTYVQV LSFPPETTIS VPLPHIYLAE LLQGGQSPFQ ATASCHIVSV FSLQLFWVCA YCTSICRQGK CTRLGSTCPT QTAISQAIIR LLVEDGTAEA VVTCRNHHVA AALGLCPREW ASLLDFVQVP GRVVLQFAGP GAQLESSARV DEPMTMFLWT LCTSPSVLRP IVLSFELERK PSKIVPLEPP RLQRFQCGEL PFLTHVNPRL RLSCLSIRES EYSSSLGILA SSC |
-Macromolecule #2: Homo sapien STN1
Macromolecule | Name: Homo sapien STN1 / type: protein_or_peptide / ID: 2 / Details: 6xHIS-STN1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
Sequence | String: MHHHHHHQPG SSRCEEETPS LLWGLDPVFL AFAKLYIRDI LDMKESRQVP GVFLYNGHPI KQVDVLGTVI GVRERDAFYS YGVDDSTGVI NCICWKKLNT ESVSAAPSAA RELSLTSQLK KLQETIEQKT KIEIGDTIRV RGSIRTYREE REIHATTYYK VDDPVWNIQI ...String: MHHHHHHQPG SSRCEEETPS LLWGLDPVFL AFAKLYIRDI LDMKESRQVP GVFLYNGHPI KQVDVLGTVI GVRERDAFYS YGVDDSTGVI NCICWKKLNT ESVSAAPSAA RELSLTSQLK KLQETIEQKT KIEIGDTIRV RGSIRTYREE REIHATTYYK VDDPVWNIQI ARMLELPTIY RKVYDQPFHS SALEKEEALS NPGALDLPSL TSLLSEKAKE FLMENRVQSF YQQELEMVES LLSLANQPVI HSASSDQVNF KKDTTSKAIH SIFKNAIQLL QEKGLVFQKD DGFDNLYYVT REDKDLHRKI HRIIQQDCQK PNHMEKGCHF LHILACARLS IRPGLSEAVL QQVLELLEDQ SDIVSTMEHY YTAF |
-Macromolecule #3: Homo sapien TEN1
Macromolecule | Name: Homo sapien TEN1 / type: protein_or_peptide / ID: 3 / Details: 6xHIS-TEN1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
Sequence | String: MSYYHHHHHH DYDIPTTENL YFQGAMGSMS QLVVDICKLN QVLLNLNERS KEIFTNSSRR FRVVAQLLDI QPYDDEENEG FLCKLIVGNL PDFHAQILDD VAHFTQVKNV RLELYVSESL YRSTFDINCS NRTPELYDAV DLTVAIWNNG FINIKCEVID IEILNLEEVN ...String: MSYYHHHHHH DYDIPTTENL YFQGAMGSMS QLVVDICKLN QVLLNLNERS KEIFTNSSRR FRVVAQLLDI QPYDDEENEG FLCKLIVGNL PDFHAQILDD VAHFTQVKNV RLELYVSESL YRSTFDINCS NRTPELYDAV DLTVAIWNNG FINIKCEVID IEILNLEEVN QLREFIASPM GQEFLQLSNS TGDYSQPNT |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TECNAI ARCTICA |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 53.6 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |