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- PDB-6qj2: Crystal structure of the C. thermophilum condensin Smc4 ATPase he... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6qj2 | ||||||
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Title | Crystal structure of the C. thermophilum condensin Smc4 ATPase head in complex with the C-terminal domain of Brn1 | ||||||
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![]() | CELL CYCLE / condensin / SMC complex / ATPase / chromosome condensation / loop extrusion | ||||||
Function / homology | ![]() condensin complex / mitotic chromosome condensation / chromosome organization / chromosome segregation / chromosome / mitotic cell cycle / cell division / chromatin binding / regulation of DNA-templated transcription / ATP hydrolysis activity ...condensin complex / mitotic chromosome condensation / chromosome organization / chromosome segregation / chromosome / mitotic cell cycle / cell division / chromatin binding / regulation of DNA-templated transcription / ATP hydrolysis activity / DNA binding / ATP binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hassler, M. / Haering, C.H. | ||||||
Funding support | 1items
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![]() | ![]() Title: Structural Basis of an Asymmetric Condensin ATPase Cycle. Authors: Hassler, M. / Shaltiel, I.A. / Kschonsak, M. / Simon, B. / Merkel, F. / Tharichen, L. / Bailey, H.J. / Macosek, J. / Bravo, S. / Metz, J. / Hennig, J. / Haering, C.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 196.3 KB | Display | ![]() |
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PDB format | ![]() | 156 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 461.7 KB | Display | ![]() |
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Full document | ![]() | 468.2 KB | Display | |
Data in XML | ![]() | 18 KB | Display | |
Data in CIF | ![]() | 23.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6q6eC ![]() 6qj0C ![]() 6qj1C ![]() 6qj3C ![]() 6qj4C ![]() 1w1wS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 44726.895 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0017120 / Cell line (production host): Sf21 / Production host: ![]() ![]() |
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#2: Protein | Mass: 15380.184 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0053810 / Cell line (production host): Sf21 / Production host: ![]() ![]() |
#3: Chemical | ChemComp-SO4 / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 61.51 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 3 % (v/v) EtOH, 0.1 M Na citrate pH 6.0, 1.5 M LiSO4 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 15, 2015 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.9→114.946 Å / Num. obs: 16953 / % possible obs: 99.9 % / Redundancy: 6.9 % / Rpim(I) all: 0.035 / Rrim(I) all: 0.093 / Rsym value: 0.086 / Net I/av σ(I): 4.1 / Net I/σ(I): 12.4 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1W1W Resolution: 3→43.445 Å / SU ML: 0.43 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 26.99
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 316.08 Å2 / Biso mean: 125.0163 Å2 / Biso min: 55.86 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3→43.445 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 5 / % reflection obs: 100 %
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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