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Yorodumi- PDB-4hdq: Crystal Structure of the Ternary Complex of KRIT1 bound to both t... -
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-Basic information
Entry | Database: PDB / ID: 4hdq | ||||||
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Title | Crystal Structure of the Ternary Complex of KRIT1 bound to both the Rap1 GTPase and the Heart of Glass (HEG1) cytoplasmic tail | ||||||
Components |
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Keywords | SIGNALING PROTEIN / RA binding motif / GTPase / HEG1 cytoplasmic tail / PTD domain / Rap1 effector / transmembrane protein / Rap1 / HEG1 / Cell-cell junctions / plasma membrane / nucleus | ||||||
Function / homology | Function and homology information lymph circulation / cardiac muscle tissue growth / negative regulation of membrane permeability / negative regulation of Rho-dependent protein serine/threonine kinase activity / venous blood vessel morphogenesis / regulation of body fluid levels / Rap protein signal transduction / protein localization to cell junction / positive regulation of fibroblast growth factor production / modification of postsynaptic structure ...lymph circulation / cardiac muscle tissue growth / negative regulation of membrane permeability / negative regulation of Rho-dependent protein serine/threonine kinase activity / venous blood vessel morphogenesis / regulation of body fluid levels / Rap protein signal transduction / protein localization to cell junction / positive regulation of fibroblast growth factor production / modification of postsynaptic structure / GTPase regulator activity / pericardium development / regulation of cell junction assembly / endothelium development / negative regulation of calcium ion-dependent exocytosis / positive regulation of integrin activation / cardiac atrium morphogenesis / endothelial cell morphogenesis / lymph vessel development / negative regulation of synaptic vesicle exocytosis / calcium-ion regulated exocytosis / integrin activation / cell-cell junction organization / ventricular trabecula myocardium morphogenesis / Rap1 signalling / negative regulation of endothelial cell migration / establishment of endothelial barrier / regulation of establishment of cell polarity / MET activates RAP1 and RAC1 / negative regulation of Rho protein signal transduction / azurophil granule membrane / ventricular septum development / small GTPase-mediated signal transduction / negative regulation of endothelial cell proliferation / p130Cas linkage to MAPK signaling for integrins / GRB2:SOS provides linkage to MAPK signaling for Integrins / vasculogenesis / regulation of angiogenesis / negative regulation of endothelial cell apoptotic process / cellular response to cAMP / phosphatidylinositol-4,5-bisphosphate binding / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / lipid droplet / Integrin signaling / negative regulation of angiogenesis / post-embryonic development / cell redox homeostasis / small monomeric GTPase / G protein activity / establishment of localization in cell / lung development / Signaling by high-kinase activity BRAF mutants / multicellular organism growth / MAP2K and MAPK activation / GDP binding / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / cell-cell junction / Signaling by BRAF and RAF1 fusions / heart development / microtubule binding / angiogenesis / in utero embryonic development / cell population proliferation / positive regulation of ERK1 and ERK2 cascade / cytoskeleton / external side of plasma membrane / GTPase activity / glutamatergic synapse / calcium ion binding / Neutrophil degranulation / protein-containing complex binding / GTP binding / protein-containing complex / extracellular space / extracellular exosome / extracellular region / membrane / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.95 Å | ||||||
Authors | Gingras, A.R. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2013 Title: The Structure of the Ternary Complex of Krev Interaction Trapped 1 (KRIT1) Bound to Both the Rap1 GTPase and the Heart of Glass (HEG1) Cytoplasmic Tail. Authors: Gingras, A.R. / Puzon-McLaughlin, W. / Ginsberg, M.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4hdq.cif.gz | 117.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4hdq.ent.gz | 87 KB | Display | PDB format |
PDBx/mmJSON format | 4hdq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hd/4hdq ftp://data.pdbj.org/pub/pdb/validation_reports/hd/4hdq | HTTPS FTP |
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-Related structure data
Related structure data | 4hdoSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 37372.348 Da / Num. of mol.: 1 / Fragment: FERM domain (UNP residues 417-736) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KRIT1, CCM1 / Plasmid: pLEICS-07 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 Star (DE3) / References: UniProt: O00522 |
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#2: Protein | Mass: 19020.508 Da / Num. of mol.: 1 / Fragment: UNP residues 1-167 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RAP1B, OK/SW-cl.11 / Plasmid: pTAC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 Star (DE3) / References: UniProt: P61224 |
-Protein/peptide , 1 types, 1 molecules C
#3: Protein/peptide | Mass: 3170.456 Da / Num. of mol.: 1 / Fragment: C-terminal domain (UNP residues 1356-1381) / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: Q9ULI3 |
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-Non-polymers , 4 types, 96 molecules
#4: Chemical | ChemComp-GOL / |
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#5: Chemical | ChemComp-MG / |
#6: Chemical | ChemComp-GNP / |
#7: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.34 % |
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Crystal grow | Temperature: 278 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 15% PEG2000 MME, 100 mM Tris, 100 mM potassium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I24 / Wavelength: 0.979 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 3, 2010 |
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→30 Å / Num. all: 36963 / Num. obs: 36963 / % possible obs: 99.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.3 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 20.99 |
Reflection shell | Resolution: 1.95→2.07 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.347 / Mean I/σ(I) obs: 3.9 / Num. unique all: 16656 / % possible all: 96.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4HDO Resolution: 1.95→29.15 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.929 / SU B: 4.33 / SU ML: 0.124 / Cross valid method: THROUGHOUT / ESU R: 0.183 / ESU R Free: 0.177 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.365 Å2
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Refinement step | Cycle: LAST / Resolution: 1.95→29.15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.95→2 Å / Total num. of bins used: 20
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