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Yorodumi- PDB-6q6e: Structural and functional insights into the condensin ATPase cycle -
+Open data
-Basic information
Entry | Database: PDB / ID: 6q6e | ||||||
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Title | Structural and functional insights into the condensin ATPase cycle | ||||||
Components | Condensin complex subunit 2,Structural maintenance of chromosomes protein,Structural maintenance of chromosomes protein | ||||||
Keywords | STRUCTURAL PROTEIN / Condensin / cohesin / SMC protein complex | ||||||
Function / homology | Function and homology information condensin complex / mitotic chromosome condensation / chromosome condensation / chromosome / cell division / ATP hydrolysis activity / ATP binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Chaetomium thermophilum (fungus) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Simon, B. / Hassler, M. / Haering, C.H. / Hennig, J. | ||||||
Citation | Journal: Mol.Cell / Year: 2019 Title: Structural Basis of an Asymmetric Condensin ATPase Cycle. Authors: Hassler, M. / Shaltiel, I.A. / Kschonsak, M. / Simon, B. / Merkel, F. / Tharichen, L. / Bailey, H.J. / Macosek, J. / Bravo, S. / Metz, J. / Hennig, J. / Haering, C.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6q6e.cif.gz | 769.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6q6e.ent.gz | 661.3 KB | Display | PDB format |
PDBx/mmJSON format | 6q6e.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/q6/6q6e ftp://data.pdbj.org/pub/pdb/validation_reports/q6/6q6e | HTTPS FTP |
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-Related structure data
Related structure data | 6qj0C 6qj1C 6qj2C 6qj3C 6qj4C C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 25200.189 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (fungus) / Gene: CTHT_0053810, CTHT_0033000 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta pLysS / References: UniProt: G0SBJ6, UniProt: G0S5H7 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Details: 10 mM Na/K phosphate, 50 mM NaCl, 1 mM DTT / Ionic strength: 50 mM / Label: conditions_1 / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |