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- PDB-5ihl: STRUCTURE OF THE EXTRACELLULAR DOMAIN OF THE CD40 IN COMPLEX WITH... -
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Basic information
Entry | Database: PDB / ID: 5ihl | ||||||
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Title | STRUCTURE OF THE EXTRACELLULAR DOMAIN OF THE CD40 IN COMPLEX WITH 3H56-5 DAB | ||||||
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![]() | IMMUNE SYSTEM/SIGNALING PROTEIN / CELL SURFACE RECEPTOR / DOMAIN ANTIBODY / ANTITUMOR / PROTEIN/PROTEIN INTERACTION / IMMUNE SYSTEM-SIGNALING PROTEIN complex | ||||||
Function / homology | ![]() cellular response to erythropoietin / B cell mediated immunity / immune response-regulating cell surface receptor signaling pathway / response to peptide / positive regulation of protein kinase C signaling / varicosity / CD40 signaling pathway / TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway / positive regulation of isotype switching to IgG isotypes / CD40 receptor complex ...cellular response to erythropoietin / B cell mediated immunity / immune response-regulating cell surface receptor signaling pathway / response to peptide / positive regulation of protein kinase C signaling / varicosity / CD40 signaling pathway / TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway / positive regulation of isotype switching to IgG isotypes / CD40 receptor complex / positive regulation of endothelial cell apoptotic process / B cell activation / response to cobalamin / B cell proliferation / defense response to protozoan / response to type II interferon / cellular response to interleukin-1 / positive regulation of blood vessel endothelial cell migration / positive regulation of B cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / positive regulation of interleukin-12 production / phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of GTPase activity / antigen binding / TNFR2 non-canonical NF-kB pathway / positive regulation of MAP kinase activity / platelet activation / intracellular calcium ion homeostasis / cellular response to mechanical stimulus / positive regulation of angiogenesis / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / cellular response to tumor necrosis factor / signaling receptor activity / positive regulation of NF-kappaB transcription factor activity / protein-containing complex assembly / cellular response to lipopolysaccharide / positive regulation of canonical NF-kappaB signal transduction / defense response to virus / inflammatory response / positive regulation of protein phosphorylation / protein domain specific binding / external side of plasma membrane / intracellular membrane-bounded organelle / neuronal cell body / ubiquitin protein ligase binding / enzyme binding / cell surface / positive regulation of transcription by RNA polymerase II / extracellular exosome / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Sheriff, S. | ||||||
![]() | ![]() Title: Functional Antagonism of Human CD40 Achieved by Targeting a Unique Species-Specific Epitope. Authors: Yamniuk, A.P. / Suri, A. / Krystek, S.R. / Tamura, J. / Ramamurthy, V. / Kuhn, R. / Carroll, K. / Fleener, C. / Ryseck, R. / Cheng, L. / An, Y. / Drew, P. / Grant, S. / Suchard, S.J. / ...Authors: Yamniuk, A.P. / Suri, A. / Krystek, S.R. / Tamura, J. / Ramamurthy, V. / Kuhn, R. / Carroll, K. / Fleener, C. / Ryseck, R. / Cheng, L. / An, Y. / Drew, P. / Grant, S. / Suchard, S.J. / Nadler, S.G. / Bryson, J.W. / Sheriff, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 437.2 KB | Display | ![]() |
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PDB format | ![]() | 361.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5dmiC ![]() 5dmjC ![]() 1jmaS ![]() 1ncfS ![]() 2aw2S ![]() 2uwiS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20084.449 Da / Num. of mol.: 4 / Fragment: Extra Cellular Domain (UNP residues 23-193) Mutation: Protein produced with Asn 153 & Asn 180, but deglycosylated with PNGase F, which leaves Asp at those positions Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Antibody | Mass: 13272.805 Da / Num. of mol.: 4 / Fragment: 3H56-5 dAb Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Chemical | ChemComp-SO4 / Sequence details | THE AUTHOR STATES THAT THE PROTEIN WAS EXPRESSED WITH ASN153 AND ASN180 (AND POST-TRANSLATIONAL ...THE AUTHOR STATES THAT THE PROTEIN WAS EXPRESSED WITH ASN153 AND ASN180 (AND POST-TRANSLATIO | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.66 Å3/Da / Density % sol: 73.61 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 3.5 Details: 80mM Tris-HCl, pH 8.5, 1.6M Ammonium dihydrogen phosphate, 20% (v/v) Glycerol |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 92 / Detector: CCD / Date: Oct 4, 2009 / Details: MICROMAX CONFOCAL |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 3.3→50 Å / Num. obs: 37772 / % possible obs: 99.8 % / Observed criterion σ(I): 0 / Redundancy: 42.5 % / Biso Wilson estimate: 53.11 Å2 / Rmerge(I) obs: 0.137 / Net I/σ(I): 33.7 |
Reflection shell | Resolution: 3.3→3.36 Å / Redundancy: 43.6 % / Rmerge(I) obs: 0.439 / Mean I/σ(I) obs: 12.5 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: CD40 ENSEMBLE FROM 1JMA, 2UWI, 2AW2, 1NCF Resolution: 3.3→29.04 Å / Cor.coef. Fo:Fc: 0.88 / Cor.coef. Fo:Fc free: 0.836 / Rfactor Rfree error: 0 / SU R Cruickshank DPI: 2.443 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 2.045 / SU Rfree Blow DPI: 0.486 / SU Rfree Cruickshank DPI: 0.496
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Displacement parameters | Biso mean: 79.59 Å2
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Refine analyze | Luzzati coordinate error obs: 0.55 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.3→29.04 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.3→3.4 Å / Total num. of bins used: 17
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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