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- PDB-6b4h: Crystal structure of Chaetomium thermophilum Gle1 CTD-Nup42 GBM-I... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6b4h | ||||||
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Title | Crystal structure of Chaetomium thermophilum Gle1 CTD-Nup42 GBM-IP6 complex | ||||||
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![]() | TRANSPORT PROTEIN / Complex / Nuclear Pore Complex / mRNA export / DEAD-box helicase | ||||||
Function / homology | ![]() poly(A)+ mRNA export from nucleus / mRNA transport / nuclear pore / protein transport / nuclear membrane / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lin, D.H. / Correia, A.R. / Cai, S.W. / Huber, F.M. / Jette, C.A. / Hoelz, A. | ||||||
![]() | ![]() Title: Structural and functional analysis of mRNA export regulation by the nuclear pore complex. Authors: Lin, D.H. / Correia, A.R. / Cai, S.W. / Huber, F.M. / Jette, C.A. / Hoelz, A. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 311.7 KB | Display | ![]() |
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PDB format | ![]() | 258.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.2 MB | Display | ![]() |
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Full document | ![]() | 3.2 MB | Display | |
Data in XML | ![]() | 32.1 KB | Display | |
Data in CIF | ![]() | 45.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6b4eC ![]() 6b4fC ![]() 6b4gC ![]() 6b4iC ![]() 6b4jC ![]() 6b4kC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 2 types, 4 molecules DBAC
#1: Protein | Mass: 8425.326 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 35483.184 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 4 types, 364 molecules ![](data/chem/img/IHP.gif)
![](data/chem/img/MES.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MES.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-IHP / #4: Chemical | #5: Chemical | ChemComp-ZN / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.83 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop Details: 10 mM zinc sulfate heptahydrate, 0.1 M MES pH 6.3, 18 % (w/v) PEG MME 550 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jan 7, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 2.17→35 Å / Num. obs: 52814 / % possible obs: 98.4 % / Redundancy: 6.7 % / CC1/2: 0.999 / Rpim(I) all: 0.043 / Rsym value: 0.111 / Net I/σ(I): 11.1 |
Reflection shell | Resolution: 2.17→2.25 Å / Redundancy: 6.9 % / Mean I/σ(I) obs: 1.9 / Num. unique obs: 5249 / CC1/2: 0.87 / Rpim(I) all: 0.498 / Rsym value: 1.316 / % possible all: 98.5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.17→34.847 Å
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Refine LS restraints |
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LS refinement shell |
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