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Yorodumi- PDB-6b4e: Crystal structure of Saccharomyces cerevisiae Gle1 CTD-Nup42 GBM ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6b4e | |||||||||||||||||||||
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Title | Crystal structure of Saccharomyces cerevisiae Gle1 CTD-Nup42 GBM complex | |||||||||||||||||||||
Components |
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Keywords | TRANSPORT PROTEIN / Complex / Nuclear Pore Complex / mRNA export / DEAD-box helicase | |||||||||||||||||||||
Function / homology | Function and homology information cellular response to salt stress / mRNA export from nucleus in response to heat stress / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore central transport channel / regulation of translational termination / tRNA export from nucleus / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore cytoplasmic filaments / inositol hexakisphosphate binding / structural constituent of nuclear pore ...cellular response to salt stress / mRNA export from nucleus in response to heat stress / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore central transport channel / regulation of translational termination / tRNA export from nucleus / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore cytoplasmic filaments / inositol hexakisphosphate binding / structural constituent of nuclear pore / regulation of translational initiation / nucleocytoplasmic transport / poly(A)+ mRNA export from nucleus / NLS-bearing protein import into nucleus / enzyme activator activity / mRNA export from nucleus / translation initiation factor binding / nuclear pore / phospholipid binding / mRNA processing / transcription corepressor activity / protein transport / nuclear envelope / nuclear membrane / mitochondrion / nucleus / cytoplasm Similarity search - Function | |||||||||||||||||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | |||||||||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.75 Å | |||||||||||||||||||||
Authors | Lin, D.H. / Correia, A.R. / Cai, S.W. / Huber, F.M. / Jette, C.A. / Hoelz, A. | |||||||||||||||||||||
Funding support | United States, 6items
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Citation | Journal: Nat Commun / Year: 2018 Title: Structural and functional analysis of mRNA export regulation by the nuclear pore complex. Authors: Lin, D.H. / Correia, A.R. / Cai, S.W. / Huber, F.M. / Jette, C.A. / Hoelz, A. | |||||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6b4e.cif.gz | 490.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6b4e.ent.gz | 418.3 KB | Display | PDB format |
PDBx/mmJSON format | 6b4e.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b4/6b4e ftp://data.pdbj.org/pub/pdb/validation_reports/b4/6b4e | HTTPS FTP |
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-Related structure data
Related structure data | 6b4fC 6b4gC 6b4hC 6b4iC 6b4jC 6b4kC 3rrnS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 34258.738 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / Gene: GLE1, BRR3, RSS1, YDL207W, D1049 / Production host: Escherichia coli (E. coli) / References: UniProt: Q12315 #2: Protein/peptide | Mass: 4076.666 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) Strain: ATCC 204508 / S288c / Gene: NUP42, RIP1, UIP1, YDR192C, YD9346.04C / Production host: Escherichia coli (E. coli) / References: UniProt: P49686 #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.17 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop Details: 0.1 M HEPES pH 8.2, 11 % (w/v/) PEG 3350, 0.2 M L-Proline |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 8, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→47.3 Å / Num. obs: 85335 / % possible obs: 99.3 % / Redundancy: 21.8 % / CC1/2: 0.999 / Rpim(I) all: 0.025 / Rsym value: 0.124 / Net I/σ(I): 16.4 |
Reflection shell | Resolution: 1.75→1.81 Å / Redundancy: 10.5 % / Mean I/σ(I) obs: 1.3 / Num. unique obs: 7866 / CC1/2: 0.573 / Rpim(I) all: 0.398 / Rsym value: 1.27 / % possible all: 93.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3RRN Resolution: 1.75→47.299 Å / SU ML: 0.25 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 21.41 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.75→47.299 Å
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Refine LS restraints |
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LS refinement shell |
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