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Open data
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Basic information
Entry | Database: PDB / ID: 5x67 | ||||||
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Title | Human thymidylate synthase in complex with dUMP and nolatrexed | ||||||
![]() | Thymidylate synthase | ||||||
![]() | TRANSFERASE / methyltransferase | ||||||
Function / homology | ![]() uracil metabolic process / response to organophosphorus / intestinal epithelial cell maturation / response to folic acid / Interconversion of nucleotide di- and triphosphates / thymidylate synthase / response to vitamin A / sequence-specific mRNA binding / cartilage development / tetrahydrofolate interconversion ...uracil metabolic process / response to organophosphorus / intestinal epithelial cell maturation / response to folic acid / Interconversion of nucleotide di- and triphosphates / thymidylate synthase / response to vitamin A / sequence-specific mRNA binding / cartilage development / tetrahydrofolate interconversion / thymidylate synthase activity / folic acid binding / dTMP biosynthetic process / dTTP biosynthetic process / DNA biosynthetic process / G1/S-Specific Transcription / developmental growth / response to glucocorticoid / mRNA regulatory element binding translation repressor activity / response to cytokine / response to progesterone / liver regeneration / response to toxic substance / circadian rhythm / methylation / response to ethanol / mitochondrial inner membrane / negative regulation of translation / mitochondrial matrix / response to xenobiotic stimulus / protein homodimerization activity / mitochondrion / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chen, D. / Jansson, A. / Larsson, A. / Nordlund, P. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural analyses of human thymidylate synthase reveal a site that may control conformational switching between active and inactive states. Authors: Chen, D. / Jansson, A. / Sim, D. / Larsson, A. / Nordlund, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 133.9 KB | Display | ![]() |
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PDB format | ![]() | 103.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5x4wC ![]() 5x4xC ![]() 5x4yC ![]() 5x5aC ![]() 5x5dC ![]() 5x5qC ![]() 5x66C ![]() 5x69C ![]() 1i00S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 33254.125 Da / Num. of mol.: 2 / Fragment: UNP residues 26-313 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.71 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: 100mM sodium calcodylate, pH 6.5, 25% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 14, 2014 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.013 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 2.13→48.22 Å / Num. obs: 35533 / % possible obs: 99.5 % / Redundancy: 15.8 % / CC1/2: 0.998 / Rmerge(I) obs: 0.177 / Rpim(I) all: 0.046 / Rrim(I) all: 0.183 / Net I/σ(I): 15.5 / Num. measured all: 560607 / Scaling rejects: 0 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1I00 Resolution: 2.13→48.22 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.924 / SU B: 4.108 / SU ML: 0.108 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.198 / ESU R Free: 0.172 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 75.81 Å2 / Biso mean: 22.127 Å2 / Biso min: 2 Å2
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Refinement step | Cycle: final / Resolution: 2.13→48.22 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.135→2.19 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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