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Yorodumi- PDB-5uue: Tetragonal thermolysin cryocooled to 100 K with 50% methanol as c... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5uue | ||||||
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Title | Tetragonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant | ||||||
Components | Thermolysin | ||||||
Keywords | HYDROLASE / zinc protease / alpha/beta | ||||||
Function / homology | Function and homology information thermolysin / metalloendopeptidase activity / proteolysis / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | Bacillus thermoproteolyticus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.60000115572 Å | ||||||
Authors | Juers, D.H. | ||||||
Citation | Journal: Acta Crystallogr D Struct Biol / Year: 2018 Title: The impact of cryosolution thermal contraction on proteins and protein crystals: volumes, conformation and order. Authors: Juers, D.H. / Farley, C.A. / Saxby, C.P. / Cotter, R.A. / Cahn, J.K.B. / Holton-Burke, R.C. / Harrison, K. / Wu, Z. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5uue.cif.gz | 170.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5uue.ent.gz | 110.6 KB | Display | PDB format |
PDBx/mmJSON format | 5uue.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uu/5uue ftp://data.pdbj.org/pub/pdb/validation_reports/uu/5uue | HTTPS FTP |
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-Related structure data
Related structure data | 5un3C 5uu7C 5uu8C 5uu9C 5uuaC 5uubC 5uucC 5uudC 6avlC 6b6nC 6b6oC 6b6pC 6b6qC 6b6rC 6b6sC 6b6tC 6d5nC 6d5oC 6d5pC 6d5qC 6d5rC 6d5sC 6d5tC 6d5uC 6d6eC 6d6fC 6d6gC 6d6hC 6dzfC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 34360.336 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus thermoproteolyticus (bacteria) Gene: npr / Production host: Bacillus thermoproteolyticus (bacteria) / References: UniProt: P00800, thermolysin |
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-Non-polymers , 5 types, 428 molecules
#2: Chemical | #3: Chemical | ChemComp-ZN / #4: Chemical | ChemComp-CL / #5: Chemical | ChemComp-MOH / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.52 Å3/Da / Density % sol: 65.1 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop Details: Well: ~2 M AmSO4 Drop: 50 mg/mL protein in 45% DMSO, 0.5 M ZnCl2 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SEALED TUBE / Type: OXFORD DIFFRACTION ENHANCE ULTRA / Wavelength: 1.54 Å |
Detector | Type: OXFORD ONYX CCD / Detector: CCD / Date: Oct 30, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→20.801 Å / Num. obs: 121775 / % possible obs: 98.5 % / Redundancy: 7.3 % / Biso Wilson estimate: 19.5712077884 Å2 / CC1/2: 0.998 / Net I/σ(I): 13.1 |
Reflection shell | Highest resolution: 1.6 Å / Redundancy: 4.9 % / CC1/2: 0.178 / % possible all: 93.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.60000115572→20.8005965727 Å / SU ML: 0.257288165086 / Cross valid method: FREE R-VALUE / σ(F): 0.901022574281 / Phase error: 26.7832114386
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.8586592548 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.60000115572→20.8005965727 Å
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Refine LS restraints |
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LS refinement shell |
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