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Structure paper

TitleThe impact of cryosolution thermal contraction on proteins and protein crystals: volumes, conformation and order.
Journal, issue, pagesActa Crystallogr D Struct Biol, Vol. 74, Page 922-938, Year 2018
Publish dateJan 30, 2017 (structure data deposition date)
AuthorsJuers, D.H. / Farley, C.A. / Saxby, C.P. / Cotter, R.A. / Cahn, J.K.B. / Holton-Burke, R.C. / Harrison, K. / Wu, Z.
External linksActa Crystallogr D Struct Biol / PubMed:30198901
MethodsX-ray diffraction
Resolution1.59969199601 - 3.00010867362 Å
Structure data

PDB-5un3:
Tetragonal thermolysin (295 K) in the presence of 50% xylose
Method: X-RAY DIFFRACTION / Resolution: 1.60004437941 Å

PDB-5uu7:
Tetragonal thermolysin (295 K) in the presence of 50% mpd
Method: X-RAY DIFFRACTION / Resolution: 1.60001390701 Å

PDB-5uu8:
Tetragonal thermolysin cryocooled to 100 K with 30% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5uu9:
Tetragonal thermolysin cryocooled to 100 K with 40% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 1.59969199601 Å

PDB-5uua:
Tetragonal thermolysin cryocooled to 100 K with 50% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 1.60000384919 Å

PDB-5uub:
Tetragonal thermolysin cryocooled to 100 K with 25% xylose/25% mpd as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 1.60000288281 Å

PDB-5uuc:
Tetragonal thermolysin cryocooled to 100 K with 50% mpd as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 1.60000931163 Å

PDB-5uud:
Tetragonal thermolysin cryocooled to 100 K with 50% dmf as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 1.60000346607 Å

PDB-5uue:
Tetragonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 1.60000115572 Å

PDB-6avl:
Orthorhombic Trypsin (295 K) in the presence of 50% xylose
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-6b6n:
Orthorhombic trypsin (295 K) in the presence of 50% mpd
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-6b6o:
Orthorhombic trypsin cryocooled to 100 K with 20% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.40008089282 Å

PDB-6b6p:
Orthorhombic trypsin cryocooled to 100 K with 30% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.00005891372 Å

PDB-6b6q:
Orthorhombic trypsin cryocooled to 100 K with 50% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.00006794459 Å

PDB-6b6r:
Orthorhombic trypsin cryocooled to 100 K with 50% mpd as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.00005867689 Å

PDB-6b6s:
Orthorhombic trypsin cryocooled to 100 K with 50% ethanol as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.00022843018 Å

PDB-6b6t:
Orthorhombic trypsin cryocooled to 100 K with 50% methanol as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.00000470779 Å

PDB-6d5n:
Hexagonal thermolysin (295) in the presence of 50% xylose
Method: X-RAY DIFFRACTION / Resolution: 2.00003665634 Å

PDB-6d5o:
Hexagonal thermolysin (295 K) in the presence of 50% DMF
Method: X-RAY DIFFRACTION / Resolution: 2.00005217381 Å

PDB-6d5p:
Hexagonal thermolysin cryocooled to 100 K with 20% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 3.00010867362 Å

PDB-6d5q:
Hexagonal thermolysin cryocooled to 100 K with 30% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.0001613725 Å

PDB-6d5r:
Hexagonal thermolysin cryocooled to 100 K with 50% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.00002117296 Å

PDB-6d5s:
Hexagonal thermolysin cryocooled to 100 K with 50% MPD as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.00003547199 Å

PDB-6d5t:
Hexagonal thermolysin cryocooled to 100 K with 50% MPD as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.00003718877 Å

PDB-6d5u:
Hexagonal thermolysin cryocooled to 100 K with 50% methanol as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.00009532805 Å

PDB-6d6e:
Triclinic lysozyme (295 K) in the presence of 47% xylose
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-6d6f:
Triclinic lysozyme cryocooled to 100 K with 47% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-6d6g:
Triclinic lysozyme (295 K) in the presence of 47% MPD
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-6d6h:
Triclinic lysozyme cryocooled to 100 K with 47% MPD as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.00006383515 Å

PDB-6dzf:
Orthorhombic trypsin cryocooled to 100 K with 20% xylose as cryoprotectant
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-CL:
Unknown entry / Chloride

ChemComp-XYP:
beta-D-xylopyranose / Xylose

ChemComp-HOH:
WATER / Water

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM / 2-Methyl-2,4-pentanediol

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM / 2-Methyl-2,4-pentanediol

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM / Dimethyl sulfoxide

ChemComp-DMF:
DIMETHYLFORMAMIDE / Dimethylformamide

ChemComp-MOH:
METHANOL / Methanol

ChemComp-BEN:
BENZAMIDINE / Benzamidine

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-XYS:
alpha-D-xylopyranose / Xylose

ChemComp-EOH:
ETHANOL / Ethanol

ChemComp-VAL:
VALINE / Valine

ChemComp-LYS:
LYSINE / Lysine

ChemComp-NO3:
NITRATE ION / Nitrate

Source
  • bacillus thermoproteolyticus (bacteria)
  • bos taurus (cattle)
  • gallus gallus (chicken)
KeywordsHYDROLASE / zinc protease / alpha/beta / serine protease / Glycosidase

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