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- PDB-5onw: X-Ray crystal structure of a nucleosome core particle with its DN... -

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Basic information

Entry
Database: PDB / ID: 5onw
TitleX-Ray crystal structure of a nucleosome core particle with its DNA site-specifically crosslinked to the histone octamer and the two H2A/H2B dimers crosslinked via H2A N38C
Components
  • (DNA (147-MER)) x 2
  • Histone H2A
  • Histone H2B 1.1
  • Histone H3.2
  • Histone H4
KeywordsDNA BINDING PROTEIN / Nucleosome Core Particle / Histones / Disulfide / Convertible Nucleotide / DNA
Function / homology
Function and homology information


DNA-templated transcription, initiation / nucleosome / protein heterodimerization activity / DNA binding / nucleoplasm / nucleus
Similarity search - Function
Histone, subunit A / Histone, subunit A / Histone H2B signature. / Histone H2B / Histone H2B / Histone H2A signature. / Histone H2A conserved site / Histone H2A, C-terminal domain / C-terminus of histone H2A / Histone 2A ...Histone, subunit A / Histone, subunit A / Histone H2B signature. / Histone H2B / Histone H2B / Histone H2A signature. / Histone H2A conserved site / Histone H2A, C-terminal domain / C-terminus of histone H2A / Histone 2A / Histone H2A / Histone H4, conserved site / Histone H4 signature. / Histone H4 / Histone H4 / Centromere kinetochore component CENP-T histone fold / CENP-T/Histone H4, histone fold / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF) / Histone H3 signature 1. / Histone H3 signature 2. / Histone H3 / Histone H3/CENP-A / Histone H2A/H2B/H3 / Core histone H2A/H2B/H3/H4 / Histone-fold / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Histone H4 / Histone H3.2 / Histone H2A type 1 / : / Histone H2B 1.1 / DNA (> 100) / DNA (> 10) / DNA / Histone H2A
Similarity search - Component
Biological speciesHomo sapiens (human)
Xenopus laevis (African clawed frog)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å
AuthorsFrouws, T.D. / Barth, P.D. / Richmond, T.J.
Funding support Switzerland, 1items
OrganizationGrant numberCountry
Louis Jeantet Prize Switzerland
CitationJournal: J. Mol. Biol. / Year: 2018
Title: Site-Specific Disulfide Crosslinked Nucleosomes with Enhanced Stability.
Authors: Frouws, T.D. / Barth, P.D. / Richmond, T.J.
History
DepositionAug 4, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 22, 2017Provider: repository / Type: Initial release
Revision 1.1Dec 27, 2017Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
I: DNA (147-MER)
J: DNA (147-MER)
A: Histone H3.2
B: Histone H4
C: Histone H2A
D: Histone H2B 1.1
E: Histone H3.2
F: Histone H4
G: Histone H2A
H: Histone H2B 1.1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)200,30034
Polymers199,06010
Non-polymers1,24124
Water1,11762
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area60820 Å2
ΔGint-536 kcal/mol
Surface area71630 Å2
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)106.560, 183.830, 109.490
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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DNA chain , 2 types, 2 molecules IJ

#1: DNA chain DNA (147-MER)


Mass: 45430.148 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pUC57 / Production host: Escherichia coli (E. coli) / Strain (production host): DH10B
#2: DNA chain DNA (147-MER)


Mass: 45421.133 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pUC57 / Production host: Escherichia coli (E. coli) / Strain (production host): DH10B

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Protein , 4 types, 8 molecules AEBFCGDH

#3: Protein Histone H3.2


Mass: 15217.812 Da / Num. of mol.: 2 / Mutation: C110A, R40C
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Xenopus laevis (African clawed frog) / Plasmid: pET3a / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: P84233
#4: Protein Histone H4 /


Mass: 11394.426 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Xenopus laevis (African clawed frog) / Plasmid: pET3a / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: P62799
#5: Protein Histone H2A /


Mass: 13967.281 Da / Num. of mol.: 2 / Mutation: N38C
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Xenopus laevis (African clawed frog) / Gene: hist1h2aj, LOC494591 / Plasmid: pET3a / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: Q6AZJ8, UniProt: P06897*PLUS
#6: Protein Histone H2B 1.1 / H2B1.1


Mass: 13524.752 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Xenopus laevis (African clawed frog) / Plasmid: pET3a / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS / References: UniProt: P02281

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Non-polymers , 3 types, 86 molecules

#7: Chemical
ChemComp-MN / MANGANESE (II) ION


Mass: 54.938 Da / Num. of mol.: 20 / Source method: obtained synthetically / Formula: Mn
#8: Chemical
ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Cl
#9: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 62 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.69 Å3/Da / Density % sol: 54.34 %
Crystal growTemperature: 295 K / Method: vapor diffusion, sitting drop / pH: 6
Details: Sample was mixed in a 1:1 ratio with 10 mM Na-cacodylate, pH 6.0, 160-210 mM MnCl2, 140-200 mM KCl and equilibrated against a 1:4 dilution of the same solution

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Data collection

DiffractionMean temperature: 90 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Jun 5, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.8→29.505 Å / Num. obs: 52834 / % possible obs: 98.4 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.062 / Net I/σ(I): 13.9
Reflection shellResolution: 2.8→2.85 Å / Rmerge(I) obs: 0.328 / Mean I/σ(I) obs: 2.8 / % possible all: 93.7

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Processing

Software
NameVersionClassification
PHENIX(dev_2447: ???)refinement
XDSdata reduction
SCALAdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1kx5
Resolution: 2.8→29.505 Å / SU ML: 0.36 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 26.98
RfactorNum. reflection% reflectionSelection details
Rfree0.2544 2661 5.04 %random
Rwork0.2076 ---
obs0.21 52757 98.34 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.8→29.505 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5956 6027 24 62 12069
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00512798
X-RAY DIFFRACTIONf_angle_d0.71318544
X-RAY DIFFRACTIONf_dihedral_angle_d31.8925264
X-RAY DIFFRACTIONf_chiral_restr0.042104
X-RAY DIFFRACTIONf_plane_restr0.051472
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.7996-2.85050.34841260.29972317X-RAY DIFFRACTION89
2.8505-2.90520.35321260.26942531X-RAY DIFFRACTION95
2.9052-2.96450.27731380.24952536X-RAY DIFFRACTION95
2.9645-3.02890.30611100.25862635X-RAY DIFFRACTION99
3.0289-3.09930.31281590.262584X-RAY DIFFRACTION99
3.0993-3.17670.35631420.2712649X-RAY DIFFRACTION99
3.1767-3.26250.30551470.25572617X-RAY DIFFRACTION99
3.2625-3.35840.29911310.24372656X-RAY DIFFRACTION99
3.3584-3.46660.26491510.23192644X-RAY DIFFRACTION99
3.4666-3.59030.26171440.22352585X-RAY DIFFRACTION98
3.5903-3.73380.25471370.21642663X-RAY DIFFRACTION99
3.7338-3.90330.26891550.21622626X-RAY DIFFRACTION99
3.9033-4.10860.26881350.2062683X-RAY DIFFRACTION100
4.1086-4.36530.26741360.1912700X-RAY DIFFRACTION100
4.3653-4.70110.23211390.18432681X-RAY DIFFRACTION100
4.7011-5.17190.22561540.18722685X-RAY DIFFRACTION100
5.1719-5.9150.26891330.20152732X-RAY DIFFRACTION100
5.915-7.43260.2571590.21192723X-RAY DIFFRACTION99
7.4326-29.50660.17531390.16022849X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
10.0143-0.0074-0.02750.00830.0496-0.10770.5603-0.46690.15290.3117-0.150.1572-0.2076-0.3084-01.07050.1630.27251.0495-0.10971.07049.9577112.173720.4317
20.1313-0.4156-0.11710.2597-0.3970.3583-0.0807-0.18740.22520.5253-0.1174-0.10661.06620.2634-00.55940.0858-0.0770.63880.08710.987954.595770.327721.1079
30.4336-0.4452-0.07080.24910.441.18160.09260.08880.0233-0.1027-0.07130.1074-0.0143-0.2305-00.2953-0.06980.03160.15510.05360.465235.911496.0163-0.9578
40.1256-0.0016-0.079-0.0325-0.11330.0670.5948-0.08940.1736-0.1342-0.6033-0.21060.0720.514300.87470.12560.39321.06290.02031.503282.444192.751-9.1031
50.0294-0.0081-0.0441-0.0029-0.01720.03080.26480.05180.1488-0.178-0.6263-0.09360.2890.1951-00.7324-0.04620.21250.9090.15021.108485.1844103.2454-11.6455
60.4786-0.2185-0.08770.35910.16961.13410.06390.0987-0.041-0.1905-0.08140.08360.0287-0.2036-00.2661-0.0657-0.02090.2288-0.00990.477335.891688.9798-3.2722
70.07140.10420.1284-0.0723-0.2881-0.23870.5232-0.59010.56320.16880.2545-0.21950.03730.91160-1.688-0.2967-0.41941.24670.3031.714883.3037100.346414.4734
80.2019-0.0137-0.34730.18310.0820.85960.0224-0.0125-0.06170.26020.08420.12920.1674-0.2884-00.7099-0.08290.08570.53840.15140.837627.12883.068221.114
9-0.01680.005-0.0203-0.0122-0.0115-0.0270.04650.0314-0.11310.14950.1301-0.2295-0.09230.10980-0.8953-0.9121.20460.12730.56560.404672.4775110.1211-1.3117
100.0123-0.0140.01610.0103-0.00070.0042-0.0819-0.17960.02290.3253-0.18750.04550.128-0.1101-0-0.54940.028-0.17051.15150.18360.444269.872494.173520.8588
11-0.0681-0.04940.05770.0065-0.0022-0.0682-0.231-0.5970.29770.04450.5968-0.47470.51941.06330-0.0598-0.34440.04520.01140.0820.531761.6406100.00849.913
120.0062-0.01-0.0047-0.0029-0.0013-0.0033-0.0481-0.093-0.00450.07-0.13680.04810.0132-0.0970-0.3854-0.41640.25530.40980.02220.39250.2289106.969710.691
130.0034-0.04350.0108-0.0141-0.0250.02420.1834-0.26630.09140.52670.4045-0.30420.3270.31560-0.9211-0.4533-0.16130.2436-0.07250.459963.2372101.645513.949
140.03110.03810.01520.0173-0.01550.0009-0.19170.1682-0.19010.08930.00930.03160.0782-0.1428-00.27250.09780.00310.38160.04920.387860.428583.143712.2483
15-0.0026-0.0023-0.00350.0023-0.0024-0.0007-0.02090.00110.0373-0.0068-0.04440.00650.01570.011100.8109-0.01610.1870.54450.18690.623232.005466.384531.8156
16-0.005-0.00840.0020.0035-0.00410.014-0.1687-0.088-0.3177-0.02180.1631-0.0946-0.0246-0.2288-00.6553-0.16420.13390.16570.12030.279133.839570.781922.6252
17-0.00020.00180.0005-0.00450.01310.0046-0.13290.0391-0.2309-0.0546-0.2283-0.0256-0.0254-0.017600.967-0.04910.05720.54880.02460.418943.643966.660114.4326
180.02240.02480.00080.00230.00570.0553-0.0663-0.1753-0.0830.11980.05130.0880.031-0.3234-00.2729-0.03040.02290.3189-0.00240.196933.400985.530727.0478
190.0059-0-0.00110.00010.0018-0.0022-0.0698-0.0680.1356-0.013-0.051-0.00090.0119-0.0239-00.1672-0.11350.01020.37080.01590.238118.571496.963524.3115
20-0.00170.00840.00090.01320.01580.001-0.0430.0016-0.03650.0059-0.0446-0.0719-0.1113-0.0669-00.21990.0321-0.0120.29010.03230.147329.537897.798819.5922
210.0046-0.0012-0.00480.001-0.0033-0.0015-0.07260.01030.020.0245-0.05430.0015-0.0040.040300.151-0.01590.0210.24180.01650.268740.792895.116521.5138
220.0010.00020.0044-0.0001-0.00430.0070.00410.06720.0763-0.04760.03790.0413-0.00190.005100.1361-0.0006-0.0210.29990.05710.260237.556393.102411.6486
230.0170.0050.00820.00370.00640.007-0.03740.0533-0.00590.0172-0.0187-0.032-0.0097-0.0489-00.3295-0.08990.08080.1083-0.07170.22626.637195.867512.3423
24-0.00940.00120.0030.00830.00050.00030.0491-0.0141-0.11790.02360.0079-0.02610.02190.00600.3623-0.1584-0.6111-0.1876-0.6132-0.982132.2125108.640715.9697
250.00550.0010.00220.00320.00320.0034-0.0218-0.0785-0.0318-0.0219-0.0541-0.09340.0366-0.039600.72970.110.01570.7238-0.06410.487836.0611119.744414.3389
26-0.01090.01260.01130.0033-0.012-0.0010.0220.184-0.08280.1009-0.0776-0.0423-0.026-0.146401.0018-0.2599-0.00790.4429-0.02260.301621.266870.426711.2803
27-0.01130.014-0.00630.0012-0.00410.0142-0.2896-0.19410.3331-0.09970.15950.25780.1511-0.1883-0-0.0493-0.21780.34260.54630.3090.210122.804984.428328.0255
28-0.0093-0.0236-0.04930.0025-0.0523-0.02370.23830.2107-0.1943-0.2510.4379-0.38810.63990.279500.2523-0.17350.24050.04440.2169-0.170841.166978.237815.8807
290.03630.0140.0063-0.0043-0.04370.0041-0.4787-0.10420.01210.44180.343-0.14040.02120.24700.4421-0.01280.02350.28840.04580.241344.869278.95528.7936
30-00.0003-0.0012-0.0015-0.00040.00080.0122-0.0252-0.0065-0.00550.0360.0491-0.00080.0089-01.10450.14010.13730.6261-0.14480.791422.0302128.41528.7437
310.00040.00120.0015-0.00070.00050.0010.17740.0922-0.0403-0.01510.06340.21260.0558-0.1233-00.37420.06410.05030.3246-0.08360.307124.2813115.839810.8434
32-0.00740.00560.00320.0001-0.00510.00430.1104-0.1243-0.12850.0107-0.06230.221-0.05270.005700.32570.04440.01410.31960.00210.318822.4257104.07237.7
33-0.0054-0.01510.0075-0.0079-0.00860.0122-0.00570.06260.0993-0.37680.00490.0970.00970.011400.3556-0.0868-0.2717-0.0575-1.0113-1.450523.902394.1365-9.6648
340.00360.0031-0.0028-0.00190.00380.00020.0478-0.0936-0.1925-0.07130.08530.2327-0.0039-0.0142-00.42390.0517-0.01370.5019-0.06270.264123.081783.6746-10.4811
350.0025-0.0073-0.05990.00570.04590.01950.24130.11170.12970.0242-0.2043-0.0148-0.0337-0.2231-00.15710.01650.00480.1777-0.00220.206432.9445102.33093.7056
360.03350.00430.0298-0.00190.0196-0.0005-0.1040.30420.06580.0042-0.0216-0.03030.07530.106700.3530.04570.09770.2738-0.03380.375543.0659110.9468-0.0835
37-0.01270.0096-0.0091-0.0026-0.0060.0127-0.0626-0.1573-0.26960.08510.00280.0651-0.15330.125300.4450.0289-0.06510.9546-0.11280.186618.011792.962-19.2207
380.0319-0.06640.03720.0537-0.02840.01040.197-0.08290.2036-0.2235-0.155-0.0023-0.0205-0.285400.3310.1068-0.00260.2223-0.00560.259527.878110.9662-0.7268
390.01360.02340.10210.00810.0651-0.00290.09220.3630.093-0.04780.2372-0.1059-0.273-0.310400.2321-0.1227-0.04840.3219-0.08180.11331.932591.1522-6.8008
400.0195-0.00440.0070.0160.01850.01080.074-0.0842-0.1175-0.24920.0172-0.10260.1498-0.037100.14990.0217-0.0120.3038-0.02020.141932.831886.44214.4536
410.0137-0.01370.01360.0360.0050.01640.1563-0.2091-0.54580.0509-0.0454-0.29380.089-0.0106-00.70220.12750.18560.5654-0.05250.658352.71764.424-5.1836
420.023-0.04320.01460.014-0.02010.00240.37620.3857-0.1474-0.2626-0.09380.07480.23860.3443-00.3430.42150.73450.3279-0.3018-0.140957.861679.5842-13.0831
430.00280.0026-0.0005-0.0016-0.0001-0.0011-0.10260.02770.07340.0346-0.0562-0.0158-0.02430.053700.10240.31610.56470.81310.12630.78274.287489.0768-12.5675
440.02410.02430.01550.0036-0.01980.0295-0.1509-0.02910.2381-0.29490.03040.011-0.00210.041900.37420.01510.16760.42790.06340.432259.752993.5038-6.1524
450.0005-0.00130.0017-0.001-0.0026-0.0002-0.00830.01260.04110.0054-0.0097-0.01560.0467-0.0115-00.7375-0.02510.07970.65880.16510.783260.9374116.8557-8.514
46-0.0003-0.00270.0044-0.0001-0.0020.0017-0.062-0.01830.02570.0190.00310.04670.0355-0.004200.992-0.06610.16470.4365-0.0780.680364.362269.09371.1626
47-0.0010.0034-0.00090.00530.0032-0.00020.03680.05210.1041-0.00970.0495-0.0192-0.0140.033600.58410.37630.43030.53-0.26260.716866.349874.3379-12.9205
480.00180.00180.0011-0.00590.00370.006-0.0160.03730.0024-0.05930.0431-0.0480.01460.0251-00.44120.23550.5830.89110.00991.124171.416981.3516-15.1803
490.0013-0.00620.0092-0.0161-0.01420.01060.1668-0.169-0.022-0.1397-0.1352-0.08790.187-0.0822-00.45740.14220.09020.3737-0.040.468752.52777.9941-0.719
500.0002-0.00150.00150.001-0.00590.00060.04890.046-0.0263-0.06930.04440.0208-0.0264-0.0186-00.7357-0.0173-0.04790.54910.01740.761135.919867.6859-1.0945
510.0094-0.0137-0.0104-0.00290.01530.010.1446-0.0190.11220.02140.0160.0750.1059-0.0115-00.4944-0.0269-0.01880.2707-0.0760.309442.172777.3796-8.3685
520.0107-0.00630.00850.00010.00710.0283-0.13950.2990.082-0.07530.10090.05590.12050.016101.64430.1181-0.11710.0658-0.6581-0.173847.808472.4976-17.3077
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'I' and (resid -73 through -54 )
2X-RAY DIFFRACTION2chain 'I' and (resid -53 through -14 )
3X-RAY DIFFRACTION3chain 'I' and (resid -13 through 46 )
4X-RAY DIFFRACTION4chain 'I' and (resid 47 through 73 )
5X-RAY DIFFRACTION5chain 'J' and (resid -73 through -54 )
6X-RAY DIFFRACTION6chain 'J' and (resid -53 through 6 )
7X-RAY DIFFRACTION7chain 'J' and (resid 7 through 26 )
8X-RAY DIFFRACTION8chain 'J' and (resid 27 through 73 )
9X-RAY DIFFRACTION9chain 'A' and (resid 39 through 63 )
10X-RAY DIFFRACTION10chain 'A' and (resid 64 through 77 )
11X-RAY DIFFRACTION11chain 'A' and (resid 78 through 120 )
12X-RAY DIFFRACTION12chain 'A' and (resid 121 through 134 )
13X-RAY DIFFRACTION13chain 'B' and (resid 25 through 75 )
14X-RAY DIFFRACTION14chain 'B' and (resid 76 through 102 )
15X-RAY DIFFRACTION15chain 'C' and (resid 15 through 20 )
16X-RAY DIFFRACTION16chain 'C' and (resid 21 through 35 )
17X-RAY DIFFRACTION17chain 'C' and (resid 36 through 44 )
18X-RAY DIFFRACTION18chain 'C' and (resid 45 through 72 )
19X-RAY DIFFRACTION19chain 'C' and (resid 73 through 79 )
20X-RAY DIFFRACTION20chain 'C' and (resid 80 through 89 )
21X-RAY DIFFRACTION21chain 'C' and (resid 90 through 96 )
22X-RAY DIFFRACTION22chain 'C' and (resid 97 through 101 )
23X-RAY DIFFRACTION23chain 'C' and (resid 102 through 106 )
24X-RAY DIFFRACTION24chain 'C' and (resid 107 through 111 )
25X-RAY DIFFRACTION25chain 'C' and (resid 112 through 117 )
26X-RAY DIFFRACTION26chain 'D' and (resid 26 through 34 )
27X-RAY DIFFRACTION27chain 'D' and (resid 35 through 52 )
28X-RAY DIFFRACTION28chain 'D' and (resid 53 through 87 )
29X-RAY DIFFRACTION29chain 'D' and (resid 88 through 121 )
30X-RAY DIFFRACTION30chain 'E' and (resid 39 through 44 )
31X-RAY DIFFRACTION31chain 'E' and (resid 45 through 54 )
32X-RAY DIFFRACTION32chain 'E' and (resid 55 through 63 )
33X-RAY DIFFRACTION33chain 'E' and (resid 64 through 78 )
34X-RAY DIFFRACTION34chain 'E' and (resid 79 through 85 )
35X-RAY DIFFRACTION35chain 'E' and (resid 86 through 113 )
36X-RAY DIFFRACTION36chain 'E' and (resid 114 through 133 )
37X-RAY DIFFRACTION37chain 'F' and (resid 17 through 24 )
38X-RAY DIFFRACTION38chain 'F' and (resid 25 through 49 )
39X-RAY DIFFRACTION39chain 'F' and (resid 50 through 82 )
40X-RAY DIFFRACTION40chain 'F' and (resid 83 through 102 )
41X-RAY DIFFRACTION41chain 'G' and (resid 14 through 44 )
42X-RAY DIFFRACTION42chain 'G' and (resid 45 through 72 )
43X-RAY DIFFRACTION43chain 'G' and (resid 73 through 79 )
44X-RAY DIFFRACTION44chain 'G' and (resid 80 through 111 )
45X-RAY DIFFRACTION45chain 'G' and (resid 112 through 118 )
46X-RAY DIFFRACTION46chain 'H' and (resid 30 through 34 )
47X-RAY DIFFRACTION47chain 'H' and (resid 35 through 45 )
48X-RAY DIFFRACTION48chain 'H' and (resid 46 through 52 )
49X-RAY DIFFRACTION49chain 'H' and (resid 53 through 80 )
50X-RAY DIFFRACTION50chain 'H' and (resid 81 through 87 )
51X-RAY DIFFRACTION51chain 'H' and (resid 88 through 100 )
52X-RAY DIFFRACTION52chain 'H' and (resid 101 through 121 )

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