ジャーナル: Elife / 年: 2016 タイトル: Crenactin forms actin-like double helical filaments regulated by arcadin-2. 著者: Thierry Izoré / Danguole Kureisaite-Ciziene / Stephen H McLaughlin / Jan Löwe / 要旨: The similarity of eukaryotic actin to crenactin, a filament-forming protein from the crenarchaeon supports the theory of a common origin of Crenarchaea and Eukaryotes. Monomeric structures of ...The similarity of eukaryotic actin to crenactin, a filament-forming protein from the crenarchaeon supports the theory of a common origin of Crenarchaea and Eukaryotes. Monomeric structures of crenactin and actin are similar, although their filament architectures were suggested to be different. Here we report that crenactin forms double helical filaments that show exceptional similarity to eukaryotic F-actin. With cryo-electron microscopy and helical reconstruction we solved the structure of the crenactin filament to 3.8 Å resolution. When forming double filaments, the 'hydrophobic plug' loop in crenactin rearranges. Arcadin-2, also encoded by the arcade gene cluster, binds tightly with its C-terminus to the hydrophobic groove of crenactin. Binding is reminiscent of eukaryotic actin modulators such as cofilin and thymosin β4 and arcadin-2 is a depolymeriser of crenactin filaments. Our work further supports the theory of shared ancestry of Eukaryotes and Crenarchaea.
解像度: 2→2.11 Å / 冗長度: 19.7 % / Rmerge(I) obs: 1.348 / Mean I/σ(I) obs: 2.3 / CC1/2: 0.903 / % possible all: 100
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.8.0151
精密化
SCALA
データスケーリング
PHASER
位相決定
精密化
構造決定の手法: 単一同系置換・異常分散 / 解像度: 2→30 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.957 / SU B: 4.37 / SU ML: 0.115 / 交差検証法: THROUGHOUT / ESU R: 0.139 / ESU R Free: 0.128 / 詳細: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT