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TitleCrenactin forms actin-like double helical filaments regulated by arcadin-2.
Journal, issue, pagesElife, Vol. 5, Year 2016
Publish dateNov 17, 2016
AuthorsThierry Izoré / Danguole Kureisaite-Ciziene / Stephen H McLaughlin / Jan Löwe /
PubMed AbstractThe similarity of eukaryotic actin to crenactin, a filament-forming protein from the crenarchaeon supports the theory of a common origin of Crenarchaea and Eukaryotes. Monomeric structures of ...The similarity of eukaryotic actin to crenactin, a filament-forming protein from the crenarchaeon supports the theory of a common origin of Crenarchaea and Eukaryotes. Monomeric structures of crenactin and actin are similar, although their filament architectures were suggested to be different. Here we report that crenactin forms double helical filaments that show exceptional similarity to eukaryotic F-actin. With cryo-electron microscopy and helical reconstruction we solved the structure of the crenactin filament to 3.8 Å resolution. When forming double filaments, the 'hydrophobic plug' loop in crenactin rearranges. Arcadin-2, also encoded by the arcade gene cluster, binds tightly with its C-terminus to the hydrophobic groove of crenactin. Binding is reminiscent of eukaryotic actin modulators such as cofilin and thymosin β4 and arcadin-2 is a depolymeriser of crenactin filaments. Our work further supports the theory of shared ancestry of Eukaryotes and Crenarchaea.
External linksElife / PubMed:27852434 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution1.6 - 3.8 Å
Structure data

4117

5mw1

EMDB-4117, PDB-5mw1:
cryoEM structure of crenactin double helical filament at 3.8A resolution
Method: EM (helical sym.) / Resolution: 3.8 Å

PDB-5ly3:
P. calidifontis crenactin in complex with arcadin-2 C-terminal peptide
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-5ly5:
Arcadin-1 from Pyrobaculum calidifontis
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-HOH:
WATER

Source
  • pyrobaculum calidifontis (archaea)
  • Pyrobaculum calidifontis (strain JCM 11548 / VA1) (archaea)
KeywordsSTRUCTURAL PROTEIN / actin / bacterial cytoskeleton / UNKNOWN FUNCTION / arcade clauster / crenactin

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