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- PDB-5f9f: Crystal structure of RIG-I helicase-RD in complex with 24-mer blu... -

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Basic information

Entry
Database: PDB / ID: 5f9f
TitleCrystal structure of RIG-I helicase-RD in complex with 24-mer blunt-end hairpin RNA
Components
  • Probable ATP-dependent RNA helicase DDX58
  • RNA (5'-R(*GP*AP*AP*UP*AP*UP*AP*AP*UP*AP*GP*UP*GP*AP*UP*AP*UP*UP*AP*UP*AP*UP*UP*C)-3')
Keywordshydrolase/rna / Complex / RIG-I / capped RNA / self versus non-self / innate immunity / hydrolase-rna complex
Function / homology
Function and homology information


RIG-I signaling pathway / regulation of type III interferon production / cytoplasmic pattern recognition receptor signaling pathway in response to virus / positive regulation of myeloid dendritic cell cytokine production / antiviral innate immune response / OAS antiviral response / detection of virus / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / positive regulation of response to cytokine stimulus / positive regulation of granulocyte macrophage colony-stimulating factor production ...RIG-I signaling pathway / regulation of type III interferon production / cytoplasmic pattern recognition receptor signaling pathway in response to virus / positive regulation of myeloid dendritic cell cytokine production / antiviral innate immune response / OAS antiviral response / detection of virus / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / positive regulation of response to cytokine stimulus / positive regulation of granulocyte macrophage colony-stimulating factor production / TRAF3-dependent IRF activation pathway / cellular response to exogenous dsRNA / TRAF6 mediated IRF7 activation / response to exogenous dsRNA / positive regulation of interferon-alpha production / TRAF6 mediated NF-kB activation / bicellular tight junction / positive regulation of defense response to virus by host / regulation of cell migration / positive regulation of interferon-beta production / Negative regulators of DDX58/IFIH1 signaling / positive regulation of interleukin-8 production / response to virus / DDX58/IFIH1-mediated induction of interferon-alpha/beta / positive regulation of interleukin-6 production / ISG15 antiviral mechanism / ruffle membrane / positive regulation of tumor necrosis factor production / Ovarian tumor domain proteases / actin cytoskeleton / double-stranded RNA binding / ribonucleoprotein complex / positive regulation of DNA-binding transcription factor activity / single-stranded RNA binding / defense response to virus / RNA helicase / RNA helicase activity / Ub-specific processing proteases / GTP binding / ubiquitin protein ligase binding / innate immune response / positive regulation of gene expression / ATP hydrolysis activity / positive regulation of transcription by RNA polymerase II / zinc ion binding / ATP binding / identical protein binding / cytosol / cytoplasm
Similarity search - Function
phosphoenolpyruvate carboxylase, domain 3 - #30 / phosphoenolpyruvate carboxylase, domain 3 / RIG-I-like receptor, C-terminal regulatory domain / RIG-I, CARD domain repeat 2 / Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A / RIG-I-like receptor, C-terminal / RIG-I receptor C-terminal domain / C-terminal domain of RIG-I / RIG-I-like receptor, C-terminal domain superfamily / RIG-I-like receptor, C-terminal regulatory domain ...phosphoenolpyruvate carboxylase, domain 3 - #30 / phosphoenolpyruvate carboxylase, domain 3 / RIG-I-like receptor, C-terminal regulatory domain / RIG-I, CARD domain repeat 2 / Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A / RIG-I-like receptor, C-terminal / RIG-I receptor C-terminal domain / C-terminal domain of RIG-I / RIG-I-like receptor, C-terminal domain superfamily / RIG-I-like receptor, C-terminal regulatory domain / RIG-I-like receptor (RLR) C-terminal regulatory (CTR) domain profile. / Caspase recruitment domain / Caspase recruitment domain / DEAD/DEAH box helicase / DEAD/DEAH box helicase domain / Death-like domain superfamily / Helicase conserved C-terminal domain / Beta Complex / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / Helicase, C-terminal / DEAD-like helicases superfamily / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleotide triphosphate hydrolases / Up-down Bundle / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
(R,R)-2,3-BUTANEDIOL / RNA (> 10) / RNA / TRIFLUOROETHANOL / Antiviral innate immune response receptor RIG-I
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.601 Å
AuthorsWang, C. / Marcotrigiano, J. / Miller, M.T. / Jiang, F.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2016
Title: Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I.
Authors: Devarkar, S.C. / Wang, C. / Miller, M.T. / Ramanathan, A. / Jiang, F. / Khan, A.G. / Patel, S.S. / Marcotrigiano, J.
History
DepositionDec 9, 2015Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 13, 2016Provider: repository / Type: Initial release
Revision 1.1Jan 20, 2016Group: Database references / Structure summary
Revision 1.2Jan 27, 2016Group: Database references

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Probable ATP-dependent RNA helicase DDX58
B: RNA (5'-R(*GP*AP*AP*UP*AP*UP*AP*AP*UP*AP*GP*UP*GP*AP*UP*AP*UP*UP*AP*UP*AP*UP*UP*C)-3')
C: Probable ATP-dependent RNA helicase DDX58
D: RNA (5'-R(*GP*AP*AP*UP*AP*UP*AP*AP*UP*AP*GP*UP*GP*AP*UP*AP*UP*UP*AP*UP*AP*UP*UP*C)-3')
E: Probable ATP-dependent RNA helicase DDX58
F: RNA (5'-R(*GP*AP*AP*UP*AP*UP*AP*AP*UP*AP*GP*UP*GP*AP*UP*AP*UP*UP*AP*UP*AP*UP*UP*C)-3')
G: Probable ATP-dependent RNA helicase DDX58
H: RNA (5'-R(*GP*AP*AP*UP*AP*UP*AP*AP*UP*AP*GP*UP*GP*AP*UP*AP*UP*UP*AP*UP*AP*UP*UP*C)-3')
I: Probable ATP-dependent RNA helicase DDX58
J: RNA (5'-R(*GP*AP*AP*UP*AP*UP*AP*AP*UP*AP*GP*UP*GP*AP*UP*AP*UP*UP*AP*UP*AP*UP*UP*C)-3')
K: Probable ATP-dependent RNA helicase DDX58
L: RNA (5'-R(*GP*AP*AP*UP*AP*UP*AP*AP*UP*AP*GP*UP*GP*AP*UP*AP*UP*UP*AP*UP*AP*UP*UP*C)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)525,57641
Polymers523,46612
Non-polymers2,11029
Water2,270126
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area37670 Å2
ΔGint-211 kcal/mol
Surface area183650 Å2
MethodPISA
Unit cell
Length a, b, c (Å)111.524, 174.252, 308.288
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein / RNA chain , 2 types, 12 molecules ACEGIKBDFHJL

#1: Protein
Probable ATP-dependent RNA helicase DDX58 / DEAD box protein 58 / RIG-I-like receptor 1 / RLR-1 / Retinoic acid-inducible gene 1 protein / RIG- ...DEAD box protein 58 / RIG-I-like receptor 1 / RLR-1 / Retinoic acid-inducible gene 1 protein / RIG-1 / Retinoic acid-inducible gene I protein / RIG-I


Mass: 79594.734 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: DDX58 / Production host: Escherichia coli (E. coli) / References: UniProt: O95786, RNA helicase
#2: RNA chain
RNA (5'-R(*GP*AP*AP*UP*AP*UP*AP*AP*UP*AP*GP*UP*GP*AP*UP*AP*UP*UP*AP*UP*AP*UP*UP*C)-3') / 5'OH HP RNA


Mass: 7649.559 Da / Num. of mol.: 6 / Source method: obtained synthetically / Details: 5'OH HP RNA / Source: (synth.) synthetic construct (others)

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Non-polymers , 5 types, 155 molecules

#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Zn
#4: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Mg
#5: Chemical
ChemComp-ETF / TRIFLUOROETHANOL / 2,2,2-Trifluoroethanol


Mass: 100.040 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C2H3F3O
#6: Chemical
ChemComp-BU3 / (R,R)-2,3-BUTANEDIOL


Mass: 90.121 Da / Num. of mol.: 13 / Source method: isolated from a natural source / Formula: C4H10O2
#7: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 126 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 2.9 Å3/Da / Density % sol: 57.64 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop
Details: 23% (w/v) PEG 3350, 0.3 M NaSCN, 100 mM MOPS (pH 7.8)

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.918 Å
DetectorType: ADSC QUANTUM 270 / Detector: CCD / Date: Nov 18, 2012
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.918 Å / Relative weight: 1
ReflectionResolution: 2.601→30 Å / Num. obs: 166166 / % possible obs: 90.6 % / Redundancy: 13 % / Net I/σ(I): 13
Reflection shellResolution: 2.6→2.7 Å / Redundancy: 9.5 % / Mean I/σ(I) obs: 2.4 / % possible all: 80.3

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Processing

Software
NameVersionClassification
PHENIX1.9_1690refinement
iMOSFLMdata reduction
Aimlessdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5E3H
Resolution: 2.601→29.994 Å / SU ML: 0.38 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 27.98 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2594 8317 5.01 %
Rwork0.2028 --
obs0.2056 166166 90.18 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.601→29.994 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms31642 3036 114 126 34918
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00235769
X-RAY DIFFRACTIONf_angle_d0.57949037
X-RAY DIFFRACTIONf_dihedral_angle_d10.88213657
X-RAY DIFFRACTIONf_chiral_restr0.0235681
X-RAY DIFFRACTIONf_plane_restr0.0035747
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.601-2.63050.38122220.29423981X-RAY DIFFRACTION70
2.6305-2.66150.36532290.30584464X-RAY DIFFRACTION77
2.6615-2.69390.34792390.30284484X-RAY DIFFRACTION78
2.6939-2.7280.37812600.29264806X-RAY DIFFRACTION83
2.728-2.76390.34222810.27764968X-RAY DIFFRACTION87
2.7639-2.80170.33032800.27075085X-RAY DIFFRACTION87
2.8017-2.84170.33752470.25925031X-RAY DIFFRACTION87
2.8417-2.88410.31482680.26275072X-RAY DIFFRACTION87
2.8841-2.92910.32532520.26655079X-RAY DIFFRACTION87
2.9291-2.97710.33682580.27035064X-RAY DIFFRACTION88
2.9771-3.02840.32072780.27025081X-RAY DIFFRACTION88
3.0284-3.08340.3372630.26125116X-RAY DIFFRACTION88
3.0834-3.14260.31752430.24625141X-RAY DIFFRACTION88
3.1426-3.20670.30242570.23865149X-RAY DIFFRACTION89
3.2067-3.27630.30662590.2385180X-RAY DIFFRACTION89
3.2763-3.35250.28342350.22955202X-RAY DIFFRACTION89
3.3525-3.43620.26382640.21945198X-RAY DIFFRACTION90
3.4362-3.5290.27472660.2175118X-RAY DIFFRACTION88
3.529-3.63260.27412750.20835364X-RAY DIFFRACTION91
3.6326-3.74970.26352940.19765203X-RAY DIFFRACTION90
3.7497-3.88340.24923020.1895456X-RAY DIFFRACTION94
3.8834-4.03850.24533120.17825439X-RAY DIFFRACTION94
4.0385-4.22190.23133200.17535675X-RAY DIFFRACTION97
4.2219-4.44380.2143110.16385793X-RAY DIFFRACTION99
4.4438-4.72120.21362990.14655843X-RAY DIFFRACTION100
4.7212-5.08410.21893270.15255880X-RAY DIFFRACTION100
5.0841-5.59280.20923110.17315891X-RAY DIFFRACTION100
5.5928-6.39520.23993120.18925907X-RAY DIFFRACTION100
6.3952-8.03160.22083190.18176006X-RAY DIFFRACTION100
8.0316-29.99550.20523340.16696173X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
10.0225-0.0035-0.00460.03970.0180.0084-0.02210.0456-0.0058-0.03420.02240.03030.00930.0268-0.02910.08540.05420.02750.0978-0.00370.0354-52.335-26.761964.0686
20.04270.02310.03730.01890.02260.040.01490.0306-0.0517-0.00910.0042-0.00660.03180.04640.00770.06130.19840.06710.0891-0.08950.0086-48.0973-39.049170.6007
30.014-0.00340.01990.00560.01040.05940.1155-0.0421-0.00390.246-0.06660.08950.18630.03530.090.3482-0.14870.28530.08810.1011-0.0058-59.9503-32.1958105.8955
40.12450.07970.07980.04880.0210.22160.11940.02610.01160.1124-0.01160.01080.04230.08280.21430.08-0.00560.07160.0463-0.01310.0752-51.214-23.370788.9377
50.0381-0.0094-0.00070.0076-0.00320.01450.03710.0147-0.05320.0004-0.0330.04720.1119-0.0223-0.01680.476-0.10570.0940.1150.03570.2501-71.1675-55.58189.6837
60.00340.0053-0.00260.0046-0.00380.00150.0003-0.026-0.04760.0089-0.03230.01010.00910.038700.30790.0076-0.00230.21690.05550.1368-53.6605-40.629892.1723
70.00870.01140.00560.01290.00650.0058-0.0124-0.0132-0.04380.0137-0.0246-0.0310.00290.05240.01720.27920.04280.07930.1640.05010.1615-52.4834-42.20594.26
80.4069-0.1715-0.11540.2190.0770.320.39170.16610.1041-0.1968-0.07420.0544-0.145-0.08990.7372-0.04960.01270.12430.04610.00280.158-76.2172-9.824146.6894
90.4484-0.0462-0.18540.144-0.08520.28090.34360.04750.2606-0.241-0.02680.1608-0.0930.07990.6613-0.03340.13430.1840.1029-0.11340.0666-71.6212-6.307242.9905
100.0029-0.0012-0.0039-0.0001-0.00050.00550.02140.04090.0121-0.00460.03210.0226-0.0003-0.0009-00.27410.13330.0150.25050.0480.2377-77.8825-7.609637.707
110.0005-0.00060.00020.00460.00540.00610.06220.03650.03060.00490.03280.00590.018-0.0319-00.22410.06450.00670.20310.03630.2203-79.1234-6.560335.4178
120.02360.0199-0.00840.0290.0030.0067-0.02110.01520.0637-0.04230.0063-0.0176-0.0530.0351-0.01840.1189-0.07870.06570.17250.08720.2509-22.917418.387991.8382
130.0169-0.00630.01160.00250.00140.0198-0.0212-0.0170.06360.00070.05210.0141-0.00850.00540.00850.0959-0.02640.03810.1067-0.0150.2022-30.53717.2914105.2602
140.0245-0.00110.01550.00440.00170.02310.0603-0.0074-0.0250.01160.0177-0.01240.00950.03160.00440.1781-0.0055-0.00290.1720.05210.1876-34.3851-6.563894.399
150.0298-0.02010.01520.003-0.00960.0112-0.0422-0.071-0.07620.09210.0857-0.04960.06040.0126-0.02710.35810.2666-0.20760.4131-0.06250.1848-14.2211-19.2268121.2149
160.06420.03940.01410.0974-0.02690.0378-0.0462-0.05010.12380.04370.114-0.00210.04180.03470.05940.10330.02830.0040.2401-0.06120.1348-25.8396-1.3232106.3693
170.0133-0.0030.00030.0016-0.00020.018-0.04350.00270.01590.03260.0532-0.0539-0.02540.07280.00150.34290.1087-0.19160.6727-0.16350.3696-6.863310.1712129.3289
180.00070.00030.00110.00090.00160.0042-0.0017-0.02350.00550.00050.01760.0065-0.00460.016800.3064-0.01680.01080.3707-0.02190.2486-24.68121.0337117.5131
190.00310.00010.00470.00270.00240.0065-0.0058-0.00960.01280.00910.04550.02640.0019-0.004600.23630.0252-0.01860.3645-0.04180.2684-26.14730.2079119.7539
200.02170.0141-0.00910.0122-0.00870.0340.0163-0.0424-0.0489-0.0006-0.0239-0.01870.01230.0276-0.050.06260.0033-0.12240.0993-0.02470.1757-16.0298-44.981826.6269
210.0297-0.0469-0.00880.0750.00290.04480.1072-0.0813-0.06070.0407-0.0541-0.09880.00060.05880.06690.1562-0.0291-0.10270.3532-0.01760.1289-7.0819-30.73224.8003
220.0331-0.0086-0.02150.00390.00710.01010.21720.14260.15480.00220.0010.0434-0.22010.01790.2220.30.30270.32780.0906-0.2019-0.0215-28.5573-5.352610.4218
230.0191-0.0043-0.00490.00170.00780.0190.0572-0.0120.0005-0.0578-0.0024-0.0407-0.07520.04490.020.16850.08210.00130.2469-0.13380.097-7.8689-24.2649.4387
240.02850.0368-0.04530.0631-0.07430.07980.167-0.0959-0.00790.1423-0.0293-0.0583-0.0830.08010.07150.376-0.099-0.00990.4495-0.10990.2518-11.3055-16.37140.1046
250.0264-0.0195-0.00560.03460.00930.02490.0766-0.04920.050.0046-0.0041-0.0096-0.07190.03960.08110.3117-0.09420.14510.236-0.19690.1468-34.5973-10.508140.033
260.0025-0.00250.00060.0070.00420.00550.0435-0.00410.0210.01180.0154-0.0341-0.0098-0.0005-00.3382-0.1240.09980.2868-0.09060.2846-16.2452-13.866326.2159
270.0063-0.00290.00270.00510.00120.00740.038-0.00930.0051-0.0102-0.0002-0.02570.0044-0.006-0.00190.242-0.03560.01950.2795-0.12830.222-15.0576-11.426.7671
280.0756-0.0161-0.02070.0542-0.04180.06960.0779-0.13750.08050.02160.0512-0.0444-0.05290.0180.1821-0.0582-0.13940.14580.2227-0.10620.24337.056423.777887.4853
290.21930.01840.06370.0036-0.02070.05820.19140.06140.0198-0.1547-0.0191-0.0292-0.1183-0.00370.21750.39820.3140.08850.35630.04920.2177-3.607529.584347.4413
300.01-0.0230.00740.0686-0.00150.17160.12310.0343-0.0575-0.1426-0.0281-0.0032-0.0729-0.01990.1020.14250.1072-0.01620.176-0.11520.3872.325811.751760.2251
310.0077-0.0187-0.00970.03590.04010.07550.1545-0.07120.0351-0.07520.0231-0.0595-0.1570.0720.00860.2532-0.08250.13490.264-0.09250.44399.363844.145173.337
320.04780.02650.00280.01920.00010.00120.0643-0.04890.0659-0.0221-0.0055-0.0072-0.0776-0.00690.01230.40840.09160.17530.19410.03960.4833-13.998547.305567.1763
330.00370.0020.00280.0087-0.00230.00330.01080.01220.0206-0.0003-0.01760.018-0.00230.0121-0.00380.29450.10220.16870.23340.03570.33234.267333.56363.7497
340.0027-0.00420.00540.012-0.00680.00920.0090.02460.01640.033-0.0425-0.00630.03340.0389-0.0040.27030.03520.14570.19230.01150.33125.536535.274161.9457
350.0792-0.0283-0.00660.05270.04520.0552-0.1005-00.05030.0376-0.05260.0292-0.1108-0.0207-0.22060.1843-0.0145-0.09150.1571-0.11660.0662-45.5683-52.392829.0972
360.0443-0.0172-0.01210.03810.01190.0259-0.05950.0246-0.1107-0.0453-0.10570.060.12240.0476-0.13250.40470.19560.12360.20990.0120.331-36.5395-87.507210.609
370.08970.06040.00080.1755-0.03790.2016-0.12330.0284-0.1428-0.0703-0.1139-0.08630.10750.0936-0.13770.22520.05140.05050.34140.04010.2649-36.8394-74.142618.3751
380.0014-0.0001-0.00040.0031-0.00370.0035-0.046-0.0138-0.033-0.0163-0.03640.01270.0290.007900.3509-0.0288-0.02450.33870.00810.3845-43.2326-77.030222.6175
390.001-0.0020.00340.0057-0.00510.0042-0.0238-0.009-0.03540.0105-0.00330.0178-0.0164-0.0358-00.24530.0330.04410.24680.00610.3278-44.6681-79.407222.8926
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 240 through 339 )
2X-RAY DIFFRACTION2chain 'A' and (resid 340 through 419 )
3X-RAY DIFFRACTION3chain 'A' and (resid 420 through 624 )
4X-RAY DIFFRACTION4chain 'A' and (resid 625 through 793 )
5X-RAY DIFFRACTION5chain 'A' and (resid 794 through 922 )
6X-RAY DIFFRACTION6chain 'B' and (resid 1 through 10 )
7X-RAY DIFFRACTION7chain 'B' and (resid 11 through 24 )
8X-RAY DIFFRACTION8chain 'C' and (resid 241 through 600 )
9X-RAY DIFFRACTION9chain 'C' and (resid 601 through 922 )
10X-RAY DIFFRACTION10chain 'D' and (resid 1 through 10 )
11X-RAY DIFFRACTION11chain 'D' and (resid 11 through 24 )
12X-RAY DIFFRACTION12chain 'E' and (resid 242 through 339 )
13X-RAY DIFFRACTION13chain 'E' and (resid 340 through 433 )
14X-RAY DIFFRACTION14chain 'E' and (resid 434 through 469 )
15X-RAY DIFFRACTION15chain 'E' and (resid 470 through 649 )
16X-RAY DIFFRACTION16chain 'E' and (resid 650 through 819 )
17X-RAY DIFFRACTION17chain 'E' and (resid 820 through 922 )
18X-RAY DIFFRACTION18chain 'F' and (resid 1 through 10 )
19X-RAY DIFFRACTION19chain 'F' and (resid 11 through 24 )
20X-RAY DIFFRACTION20chain 'G' and (resid 240 through 347 )
21X-RAY DIFFRACTION21chain 'G' and (resid 348 through 469 )
22X-RAY DIFFRACTION22chain 'G' and (resid 470 through 696 )
23X-RAY DIFFRACTION23chain 'G' and (resid 697 through 770 )
24X-RAY DIFFRACTION24chain 'G' and (resid 771 through 819 )
25X-RAY DIFFRACTION25chain 'G' and (resid 820 through 922 )
26X-RAY DIFFRACTION26chain 'H' and (resid 1 through 10 )
27X-RAY DIFFRACTION27chain 'H' and (resid 11 through 24 )
28X-RAY DIFFRACTION28chain 'I' and (resid 240 through 433 )
29X-RAY DIFFRACTION29chain 'I' and (resid 434 through 600 )
30X-RAY DIFFRACTION30chain 'I' and (resid 601 through 770 )
31X-RAY DIFFRACTION31chain 'I' and (resid 771 through 819 )
32X-RAY DIFFRACTION32chain 'I' and (resid 820 through 922 )
33X-RAY DIFFRACTION33chain 'J' and (resid 1 through 10 )
34X-RAY DIFFRACTION34chain 'J' and (resid 11 through 24 )
35X-RAY DIFFRACTION35chain 'K' and (resid 242 through 433 )
36X-RAY DIFFRACTION36chain 'K' and (resid 434 through 592 )
37X-RAY DIFFRACTION37chain 'K' and (resid 593 through 923 )
38X-RAY DIFFRACTION38chain 'L' and (resid 1 through 10 )
39X-RAY DIFFRACTION39chain 'L' and (resid 11 through 24 )

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