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- PDB-5ewx: Fusion protein of T4 lysozyme and B4 domain of protein A from sta... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5ewx | ||||||
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Title | Fusion protein of T4 lysozyme and B4 domain of protein A from staphylococcal aureus with chemical cross-linker EY-CBS | ||||||
![]() | Endolysin,Immunoglobulin G-binding protein A,Endolysin | ||||||
![]() | PROTEIN BINDING / Fusion / EY-CBS / alpha helix / cross-linker | ||||||
Function / homology | ![]() IgG binding / viral release from host cell by cytolysis / peptidoglycan catabolic process / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / host cell cytoplasm / defense response to bacterium / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jeong, W.H. / Lee, H. / Song, D.H. / Lee, J.O. | ||||||
![]() | ![]() Title: Connecting two proteins using a fusion alpha helix stabilized by a chemical cross linker. Authors: Jeong, W.H. / Lee, H. / Song, D.H. / Eom, J.H. / Kim, S.C. / Lee, H.S. / Lee, H. / Lee, J.O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 185.7 KB | Display | ![]() |
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PDB format | ![]() | 147.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 976.5 KB | Display | ![]() |
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Full document | ![]() | 986.4 KB | Display | |
Data in XML | ![]() | 19.1 KB | Display | |
Data in CIF | ![]() | 25.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5cbnC ![]() 5cboC ![]() 5cocC ![]() 1deeS ![]() 1lydS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 25212.469 Da / Num. of mol.: 2 / Fragment: UNP RESIDUES 1-35, 219-266, 38-164 Mutation: A1212V, G1240A, E1258C, K1261A, L1262A, N40C, C54T, C97A, K162A Source method: isolated from a genetically manipulated source Details: The fusion protein of T4 lysozyme (residues 1-35), linker GGGGSGGGGS, protein A (B4 domain, residues 212-266), and T4 lysozyme (residues 38-164) Source: (gene. exp.) ![]() ![]() ![]() Gene: spa / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Sequence details | THE AUTHOR STATES THAT THERE ARE ERRORS AT THESE POSITIONS IN THE SEQUENCE DATABASE | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.23 Å3/Da / Density % sol: 61.97 % Description: THE ENTRY CONTAINS FRIEDEL PAIRS IN I_PLUS/MINUS COLUMNS. square rod-plate |
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Crystal grow | Temperature: 277 K / Method: evaporation / pH: 7.5 / Details: 1.84M Na/K Phosphate, pH 7.5 |
-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 8, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→50 Å / Num. obs: 35397 / % possible obs: 99.1 % / Redundancy: 3.5 % / Net I/σ(I): 18.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1LYD, 1DEE Resolution: 2.6→42.745 Å / SU ML: 0.39 / Cross valid method: FREE R-VALUE / σ(F): 1.39 / Phase error: 31.46 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→42.745 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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