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- PDB-4ydv: STRUCTURE OF THE ANTIBODY 7B2 THAT CAPTURES HIV-1 VIRIONS -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 4ydv
TitleSTRUCTURE OF THE ANTIBODY 7B2 THAT CAPTURES HIV-1 VIRIONS
Components
  • HIV ANTIBODY 7B2 HEAVY CHAIN,IgG H chain
  • HIV ANTIBODY 7B2 LIGHT CHAIN,Ig kappa chain C region
  • HIV GP41 PEPTIDE GP41(596-606)
KeywordsIMMUNE SYSTEM / IGG / HIV / ANTIBODY / HIV GP41 ENVELOPE PROTEIN
Function / homology
Function and homology information


IgD immunoglobulin complex / IgA immunoglobulin complex / IgM immunoglobulin complex / IgE immunoglobulin complex / Synthesis and processing of ENV and VPU / evasion of host immune response / CD22 mediated BCR regulation / Alpha-defensins / IgG immunoglobulin complex / Fc epsilon receptor (FCERI) signaling ...IgD immunoglobulin complex / IgA immunoglobulin complex / IgM immunoglobulin complex / IgE immunoglobulin complex / Synthesis and processing of ENV and VPU / evasion of host immune response / CD22 mediated BCR regulation / Alpha-defensins / IgG immunoglobulin complex / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / Dectin-2 family / immunoglobulin complex / FCGR activation / Binding and entry of HIV virion / immunoglobulin mediated immune response / Role of LAT2/NTAL/LAB on calcium mobilization / Role of phospholipids in phagocytosis / Scavenging of heme from plasma / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / positive regulation of establishment of T cell polarity / antigen binding / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / host cell endosome membrane / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / actin filament organization / Regulation of Complement cascade / Cell surface interactions at the vascular wall / FCGR3A-mediated phagocytosis / FCERI mediated MAPK activation / B cell receptor signaling pathway / Assembly Of The HIV Virion / Budding and maturation of HIV virion / Regulation of actin dynamics for phagocytic cup formation / FCERI mediated NF-kB activation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / clathrin-dependent endocytosis of virus by host cell / blood microparticle / adaptive immune response / Potential therapeutics for SARS / viral protein processing / immune response / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / virus-mediated perturbation of host defense response / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / apoptotic process / host cell plasma membrane / structural molecule activity / virion membrane / extracellular space / extracellular exosome / extracellular region / membrane / plasma membrane
Similarity search - Function
Envelope glycoprotein Gp160 / : / Retroviral envelope protein / Retroviral envelope protein GP41-like / Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120 / Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain ...Envelope glycoprotein Gp160 / : / Retroviral envelope protein / Retroviral envelope protein GP41-like / Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120 / Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain / : / Immunoglobulin subtype / Immunoglobulin / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Immunoglobulin kappa constant / Envelope glycoprotein gp160 / IgG H chain
Similarity search - Component
Biological speciesHomo sapiens (human)
Human immunodeficiency virus 1
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å
AuthorsNicely, N.I. / Pemble IV, C.W.
CitationJournal: Plos Pathog. / Year: 2015
Title: Human Non-neutralizing HIV-1 Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques.
Authors: Santra, S. / Tomaras, G.D. / Warrier, R. / Nicely, N.I. / Liao, H.X. / Pollara, J. / Liu, P. / Alam, S.M. / Zhang, R. / Cocklin, S.L. / Shen, X. / Duffy, R. / Xia, S.M. / Schutte, R.J. / ...Authors: Santra, S. / Tomaras, G.D. / Warrier, R. / Nicely, N.I. / Liao, H.X. / Pollara, J. / Liu, P. / Alam, S.M. / Zhang, R. / Cocklin, S.L. / Shen, X. / Duffy, R. / Xia, S.M. / Schutte, R.J. / Pemble Iv, C.W. / Dennison, S.M. / Li, H. / Chao, A. / Vidnovic, K. / Evans, A. / Klein, K. / Kumar, A. / Robinson, J. / Landucci, G. / Forthal, D.N. / Montefiori, D.C. / Kaewkungwal, J. / Nitayaphan, S. / Pitisuttithum, P. / Rerks-Ngarm, S. / Robb, M.L. / Michael, N.L. / Kim, J.H. / Soderberg, K.A. / Giorgi, E.E. / Blair, L. / Korber, B.T. / Moog, C. / Shattock, R.J. / Letvin, N.L. / Schmitz, J.E. / Moody, M.A. / Gao, F. / Ferrari, G. / Shaw, G.M. / Haynes, B.F.
History
DepositionFeb 23, 2015Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 12, 2015Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2021Group: Derived calculations / Source and taxonomy / Category: entity_src_gen / pdbx_struct_oper_list
Item: _entity_src_gen.pdbx_host_org_cell_line / _entity_src_gen.pdbx_host_org_strain / _pdbx_struct_oper_list.symmetry_operation
Revision 1.2Oct 23, 2024Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / diffrn_radiation_wavelength / pdbx_entry_details / pdbx_modification_feature / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
P: HIV GP41 PEPTIDE GP41(596-606)
Q: HIV GP41 PEPTIDE GP41(596-606)
L: HIV ANTIBODY 7B2 LIGHT CHAIN,Ig kappa chain C region
H: HIV ANTIBODY 7B2 HEAVY CHAIN,IgG H chain
A: HIV ANTIBODY 7B2 LIGHT CHAIN,Ig kappa chain C region
B: HIV ANTIBODY 7B2 HEAVY CHAIN,IgG H chain


Theoretical massNumber of molelcules
Total (without water)115,1636
Polymers115,1636
Non-polymers00
Water1,63991
1
P: HIV GP41 PEPTIDE GP41(596-606)
L: HIV ANTIBODY 7B2 LIGHT CHAIN,Ig kappa chain C region
H: HIV ANTIBODY 7B2 HEAVY CHAIN,IgG H chain


Theoretical massNumber of molelcules
Total (without water)57,5823
Polymers57,5823
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4890 Å2
ΔGint-31 kcal/mol
Surface area20310 Å2
MethodPISA
2
Q: HIV GP41 PEPTIDE GP41(596-606)
A: HIV ANTIBODY 7B2 LIGHT CHAIN,Ig kappa chain C region
B: HIV ANTIBODY 7B2 HEAVY CHAIN,IgG H chain


Theoretical massNumber of molelcules
Total (without water)57,5823
Polymers57,5823
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4870 Å2
ΔGint-31 kcal/mol
Surface area20310 Å2
MethodPISA
Unit cell
Length a, b, c (Å)73.434, 76.281, 127.627
Angle α, β, γ (deg.)90.00, 98.06, 90.00
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11chain H and (resseq 1:127 or resseq 134:213 )
21chain B and (resseq 1:127 or resseq 134:213 )
12chain L and (resseq 2:27 or resseq 31: 210 )
22chain A and (resseq 2:27 or resseq 31: 210 )

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111GLNGLNSERSERchain H and (resseq 1:127 or resseq 134:213 )HD1 - 12722 - 161
121GLYGLYPROPROchain H and (resseq 1:127 or resseq 134:213 )HD134 - 213168 - 247
211GLNGLNSERSERchain B and (resseq 1:127 or resseq 134:213 )BF1 - 12722 - 161
221GLYGLYPROPROchain B and (resseq 1:127 or resseq 134:213 )BF134 - 213168 - 247
112ILEILEGLNGLNchain L and (resseq 2:27 or resseq 31: 210 )LC2 - 2747 - 72
122HISHISASNASNchain L and (resseq 2:27 or resseq 31: 210 )LC31 - 21082 - 261
212ILEILEGLNGLNchain A and (resseq 2:27 or resseq 31: 210 )AE2 - 2747 - 72
222HISHISASNASNchain A and (resseq 2:27 or resseq 31: 210 )AE31 - 21082 - 261

NCS ensembles :
ID
1
2

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Components

#1: Protein/peptide HIV GP41 PEPTIDE GP41(596-606)


Mass: 1193.441 Da / Num. of mol.: 2 / Fragment: HIV GP41 PEPTIDE GP41(596-606) / Source method: obtained synthetically
Details: THIS SEQUENCE OCCURS NATURALLY. A SEGMENT OF THE FULL LENGTH PROTEIN WAS SYNTHESIZED COMMERCIALLY.
Source: (synth.) Human immunodeficiency virus 1 / References: UniProt: P04578*PLUS
#2: Antibody HIV ANTIBODY 7B2 LIGHT CHAIN,Ig kappa chain C region


Mass: 29180.635 Da / Num. of mol.: 2
Fragment: HIV ANTIBODY 7B2 LIGHT CHAIN,HIV ANTIBODY 7B2 LIGHT CHAIN
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: IGKC / Cell line (production host): HEK-293T / Production host: HOMO SAPIENS (human) / References: UniProt: P01834
#3: Antibody HIV ANTIBODY 7B2 HEAVY CHAIN,IgG H chain


Mass: 27207.551 Da / Num. of mol.: 2
Fragment: HIV ANTIBODY 7B2 HEAVY CHAIN,HIV ANTIBODY 7B2 HEAVY CHAIN
Mutation: YES,YES
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK-293T / Production host: HOMO SAPIENS (human) / References: UniProt: S6B291
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 91 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.07 Å3/Da / Density % sol: 59.98 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / pH: 3.5
Details: 6% PEG 20,000, 5% 2-PROPANOL, 0.1 M CITRIC ACID PH 3.5
PH range: 3.5

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Data collection

DiffractionMean temperature: 93 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-BM / Wavelength: 1 Å
DetectorType: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Oct 20, 2011
RadiationMonochromator: SAGITALLY FOCUSED SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.7→30.8 Å / Num. obs: 39428 / % possible obs: 99.1 % / Observed criterion σ(I): 1.5 / Redundancy: 4.9 % / Rmerge(I) obs: 0.037 / Net I/σ(I): 31
Reflection shellResolution: 2.7→2.75 Å / Redundancy: 5 % / Rmerge(I) obs: 0.676 / Mean I/σ(I) obs: 1.6 / % possible all: 98.7

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Processing

Software
NameVersionClassification
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
PHENIX(PHENIX.REFINE: 1.7.2_869)refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.7→30.8 Å / SU ML: 0.77 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 25.32 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.223 1806 5.09 %
Rwork0.189 --
obs0.191 35499 91.9 %
Solvent computationShrinkage radii: 0.98 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 52.37 Å2 / ksol: 0.31 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1--2.3138 Å20 Å2-7.3595 Å2
2--7.4176 Å20 Å2
3----5.1038 Å2
Refinement stepCycle: LAST / Resolution: 2.7→30.8 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6714 0 0 91 6805
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0066890
X-RAY DIFFRACTIONf_angle_d0.9929360
X-RAY DIFFRACTIONf_dihedral_angle_d12.8492440
X-RAY DIFFRACTIONf_chiral_restr0.0651040
X-RAY DIFFRACTIONf_plane_restr0.0041200
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDTypeRms dev position (Å)
11H1675X-RAY DIFFRACTIONPOSITIONAL0.119
12B1675X-RAY DIFFRACTIONPOSITIONAL0.119
21L1599X-RAY DIFFRACTIONPOSITIONAL0.018
22A1599X-RAY DIFFRACTIONPOSITIONAL0.018
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.7-2.7730.34531170.32852135X-RAY DIFFRACTION77
2.773-2.85450.35191240.30362282X-RAY DIFFRACTION81
2.8545-2.94660.32771260.28022355X-RAY DIFFRACTION83
2.9466-3.05180.30931290.29632414X-RAY DIFFRACTION87
3.0518-3.17390.2831370.25992554X-RAY DIFFRACTION91
3.1739-3.31820.28991400.23632616X-RAY DIFFRACTION93
3.3182-3.49290.24281420.23162675X-RAY DIFFRACTION95
3.4929-3.71140.25831440.21312692X-RAY DIFFRACTION96
3.7114-3.99740.21511460.18922730X-RAY DIFFRACTION97
3.9974-4.39860.17331470.15122754X-RAY DIFFRACTION97
4.3986-5.03270.16371500.12672803X-RAY DIFFRACTION99
5.0327-6.33160.20631500.16312822X-RAY DIFFRACTION99
6.3316-30.8310.21251540.17822861X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
19.68565.84612.02478.61731.86357.94721.0042-1.0117-0.05390.7111-0.06-1.28520.89051.7325-0.94390.82610.2473-0.11431.1324-0.42531.198221.6964-1.090456.4623
27.11922.51511.76076.26122.13557.68790.1690.38210.4092-0.45070.6221-1.0619-0.32061.3688-0.67070.6748-0.11240.16080.9894-0.33590.915117.28524.693348.3813
36.95181.95871.87675.6795.73155.85680.2721-1.18370.3623-0.90510.6354-1.72140.01690.5958-0.78990.80840.0089-0.0241.045-0.21950.777112.77050.596452.7586
45.07232.84746.70772.60284.27029.34041.8262-2.2152-0.96771.2465-0.6418-1.27911.4643-0.9898-1.14581.1048-0.001-0.25681.1414-0.24660.948812.1056-1.274758.3161
51.4704-0.98231.38431.6726-0.89091.3525-0.3953-0.1343-0.40770.11380.5206-0.7196-0.52380.4704-0.12430.74840.0422-0.16241.7623-0.66341.240323.557417.216276.373
67.2946-0.5251-3.10574.02760.73155.54650.1282-0.9786-0.12260.1930.4269-0.57950.07380.5749-0.42510.75580.1652-0.23891.2256-0.20210.751511.773717.076684.702
78.8493-0.1433-4.9163.6836-3.01287.23780.0278-1.9328-0.86980.340.6321-0.55581.02931.0729-0.42480.93070.1529-0.2741.18290.13461.190816.23726.683691.2838
84.91326.2474-3.78278.9628-2.92754.7131-0.33810.0198-0.9597-0.23990.5621-1.12930.74380.5893-0.24770.64250.128-0.11671.1779-0.19830.82415.072514.740275.9215
93.2910.7940.99175.50560.93450.38430.1755-1.1953-0.67272.0892-0.189-0.5010.42150.3278-0.06381.38550.3101-0.23812.14530.08680.87478.561813.059999.6343
103.10730.3682-2.69035.57240.37612.3010.5195-0.5926-1.47950.78660.0684-2.44250.91180.5096-0.22850.99280.3946-0.32541.5617-0.24911.432324.727113.228287.4612
117.13660.9717-2.05810.21440.18612.7389-0.00990.3202-0.27890.1071-0.268-0.0462-1.0841-1.38630.18170.81620.02120.08360.6664-0.10890.647-8.99269.240656.5967
125.587-0.2228-0.17267.5395-1.23797.7243-0.0471-0.240.3006-0.09630.0967-0.0749-0.0148-0.0177-0.08380.5912-0.0370.02850.4985-0.06570.4517-3.7666-0.769149.4559
138.05030.6871-0.72885.09844.20483.64230.5431-0.2568-0.0897-0.0278-0.50840.97540.7487-0.74690.00420.5856-0.0404-0.03720.6285-0.07890.6467-11.72430.467554.6167
142.5734-0.5391-1.85813.59473.47397.23440.229-0.15160.0394-0.0440.3102-0.42050.48780.1428-0.5650.6172-0.0279-0.06560.57-0.01690.5380.2282-0.819348.6103
155.9947-1.95553.88584.98422.18955.29550.47880.35420.3271.83790.2143-1.3241-1.54392.1048-0.09871.0719-0.0802-0.09410.5425-0.1710.6684-4.50928.781564.5343
165.96594.70871.40356.38932.34064.60990.1366-1.26420.06640.8807-0.3555-0.13390.7760.15370.09710.83660.1943-0.04760.9207-0.08590.5981-1.911919.040582.1022
174.7036.2854.53078.60514.7845.4711-0.31530.2453-0.6661-0.08410.8029-1.147-0.13790.4884-0.47080.69530.0151-0.02830.9145-0.13770.59565.357620.908980.5975
186.7532.63520.93657.29862.69153.8499-0.28180.46030.0551-0.10050.6644-1.1953-0.52570.7924-0.32790.7127-0.0861-0.02650.8421-0.14780.64827.312123.691474.3467
192.5087-1.8404-1.21752.1615-0.12836.0634-0.2147-0.47110.7767-0.00890.6113-0.784-0.81040.8666-0.310.6309-0.0596-0.04580.8136-0.2010.6989.227727.055380.7509
202.587-4.51872.9028.5112-6.01474.7897-0.1734-0.17411.29540.39820.05220.1925-0.34420.92520.16480.67790.04550.07340.7669-0.17970.5532-1.371127.785478.6629
217.2517-0.77992.40970.8820.40312.44890.02030.1639-0.3536-0.5048-0.22670.21621.1622-1.21330.10440.7657-0.0385-0.11070.4844-0.11460.6483-1.9135-21.67356.369
225.722-0.676-0.70377.7290.99619.33880.08880.084-0.3777-0.09460.0371-0.2538-0.11350.2378-0.19220.5253-0.03470.00460.4292-0.04450.47371.1743-11.588514.5456
238.7599-2.1973-0.48045.74115.1464.87480.56490.32420.4652-0.7033-0.58320.5005-0.5614-0.88010.04790.68350.0262-0.05650.5706-0.04280.6112-5.1753-13.13997.6193
241.5521.16620.41161.9328-0.08525.46080.51740.0230.01830.10290.0527-0.55590.1554-0.2619-0.57990.62050.00440.04020.5357-0.04970.56914.7649-11.924716.5723
252.01396.2863-2.61393.9938-1.42182.17910.8375-0.2773-0.2224-1.27610.0183-2.1550.08192.3955-0.53240.94910.16860.20710.6242-0.01720.54094.422-20.7112-2.26
267.1164-3.8356-3.98544.85961.62594.29310.61521.62070.0258-1.1576-0.6032-0.3272-1.17480.79350.11930.97630.02950.14481.0549-0.13440.650312.6786-32.4357-16.4254
277.8909-4.7244-3.03364.48651.95494.2336-0.0494-0.012-0.0424-0.37650.4239-0.58470.03670.1175-0.31750.90830.02860.16640.74-0.1380.709818.5866-33.5712-12.5179
289.2782-3.1959-0.65742.73211.90485.2239-0.2382-0.660.27750.41520.5704-1.6847-0.32340.6116-0.44180.64510.05420.0090.737-0.08950.728418.6068-36.375-5.9783
292.51991.85940.06042.6744-1.11916.7732-0.16940.051-0.69780.16360.2941-0.88190.54891.1468-0.07910.63380.07530.06910.7824-0.20160.812222.6062-39.6228-11.6857
306.99064.14860.86748.9491-3.62853.5353-0.10460.1731-1.1001-0.09920.1749-0.1540.60550.5742-0.07970.78090.02090.00080.6094-0.14750.43511.6631-40.502-12.4194
313.9884-2.9276-2.30092.11730.69353.96970.5219-0.30121.19240.09070.1519-2.5125-0.34790.9896-0.73280.8163-0.19320.00480.986-0.25651.37327.9611-12.290814.9364
325.5307-4.5744-1.93718.0665-2.76857.95060.62330.0258-0.8198-0.1979-0.0914-0.81360.9450.2295-0.66130.5632-0.0065-0.05510.738-0.19930.700116.6784-18.015416.6344
336.1461-2.6052-2.90573.10813.69245.48160.0631-1.08570.04181.10830.5215-0.96350.53180.8778-0.6640.92360.1108-0.32281.2001-0.32111.030522.764-16.806524.5777
344.5097-4.8838-5.64085.57787.00799.1770.45620.92690.03760.7065-0.0404-1.07730.04950.3617-0.48890.5693-0.1109-0.04740.8196-0.16050.904217.9199-13.355116.388
355.0726-4.41110.46714.782-2.72956.16640.65731.40530.38360.0287-0.0111-1.13310.2404-0.0096-0.4390.858-0.00680.01010.8228-0.2431.085822.948-15.28610.6969
368.6485-0.96174.28564.8212.42533.95720.43171.43210.1437-0.42550.9367-1.58490.32722.0363-1.17350.8698-0.260.36821.2883-0.27551.176229.1361-30.5667-12.0153
371.808-0.28082.18432.9279-1.12788.30130.12971.09471.0805-1.59420.3532-2.2031-0.83011.0224-0.20381.8783-0.45130.60741.48640.05351.444432.6092-19.683-19.3424
384.7321-5.45792.72336.2975-2.5413.1183-0.4833-0.15490.9845-0.53140.7274-1.7402-0.35080.3452-0.32390.8207-0.22980.15261.1015-0.23061.121326.7205-27.6482-5.0544
395.9859-2.05655.83347.4818-3.39225.87880.30950.6-0.3452-2.44220.6734-0.7526-1.32272.6383-0.23851.8238-0.54150.66741.79930.00181.295227.6392-25.9231-29.6266
403.5462-5.83770.64039.7725-0.76525.9195-0.57081.10821.61070.06960.477-3.6208-0.43011.8285-1.10591.0525-0.61410.4281.9476-0.31041.751439.4201-26.4008-13.1761
419.261-5.9225-6.4484.07114.47545.2323-0.0675-0.24520.04180.2910.0241-0.30570.43230.32860.19980.724-0.0139-0.01210.88070.0180.61365.0036-8.594330.6278
426.89984.44984.89633.01043.26478.43990.17030.2349-0.0279-0.8784-0.003-0.5794-0.32490.4643-0.21890.7707-0.12940.04460.7955-0.04920.62384.4041-3.974435.2832
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 2:31)
2X-RAY DIFFRACTION2(chain A and resid 32:80)
3X-RAY DIFFRACTION3(chain A and resid 81:94)
4X-RAY DIFFRACTION4(chain A and resid 95:104)
5X-RAY DIFFRACTION5(chain A and resid 105:119)
6X-RAY DIFFRACTION6(chain A and resid 120:143)
7X-RAY DIFFRACTION7(chain A and resid 144:158)
8X-RAY DIFFRACTION8(chain A and resid 159:181)
9X-RAY DIFFRACTION9(chain A and resid 182:193)
10X-RAY DIFFRACTION10(chain A and resid 194:210)
11X-RAY DIFFRACTION11(chain B and resid 1:23)
12X-RAY DIFFRACTION12(chain B and resid 24:62)
13X-RAY DIFFRACTION13(chain B and resid 63:84)
14X-RAY DIFFRACTION14(chain B and resid 85:105)
15X-RAY DIFFRACTION15(chain B and resid 106:111)
16X-RAY DIFFRACTION16(chain B and resid 112:126)
17X-RAY DIFFRACTION17(chain B and resid 127:148)
18X-RAY DIFFRACTION18(chain B and resid 149:171)
19X-RAY DIFFRACTION19(chain B and resid 172:197)
20X-RAY DIFFRACTION20(chain B and resid 198:213)
21X-RAY DIFFRACTION21(chain H and resid 1:23)
22X-RAY DIFFRACTION22(chain H and resid 24:63)
23X-RAY DIFFRACTION23(chain H and resid 64:84)
24X-RAY DIFFRACTION24(chain H and resid 85:106)
25X-RAY DIFFRACTION25(chain H and resid 107:112)
26X-RAY DIFFRACTION26(chain H and resid 113:126)
27X-RAY DIFFRACTION27(chain H and resid 127:148)
28X-RAY DIFFRACTION28(chain H and resid 149:171)
29X-RAY DIFFRACTION29(chain H and resid 172:196)
30X-RAY DIFFRACTION30(chain H and resid 197:213)
31X-RAY DIFFRACTION31(chain L and resid 2:27)
32X-RAY DIFFRACTION32(chain L and resid 31:45)
33X-RAY DIFFRACTION33(chain L and resid 46:80)
34X-RAY DIFFRACTION34(chain L and resid 81:94)
35X-RAY DIFFRACTION35(chain L and resid 95:107)
36X-RAY DIFFRACTION36(chain L and resid 108:143)
37X-RAY DIFFRACTION37(chain L and resid 144:158)
38X-RAY DIFFRACTION38(chain L and resid 159:181)
39X-RAY DIFFRACTION39(chain L and resid 182:193)
40X-RAY DIFFRACTION40(chain L and resid 194:210)
41X-RAY DIFFRACTION41(chain P and resid 596:606)
42X-RAY DIFFRACTION42(chain Q and resid 596:606)

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