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- PDB-6vjt: Co-crystals of broadly neutralizing antibody with the linear epit... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6vjt | ||||||
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Title | Co-crystals of broadly neutralizing antibody with the linear epitope from Hepatitis B surface antigen | ||||||
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![]() | ANTIVIRAL PROTEIN/IMMUNE SYSTEM / Hepatitis B / HepB / Fab / Ab / antibody / ANTIVIRAL PROTEIN / ANTIVIRAL PROTEIN-IMMUNE SYSTEM complex | ||||||
Function / homology | Large envelope protein S / Major surface antigen from hepadnavirus / caveolin-mediated endocytosis of virus by host cell / membrane => GO:0016020 / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / virion membrane / Large envelope protein![]() | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Oren, D.A. / Nussenzweig, M.C. / Wang, Q. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A Combination of Human Broadly Neutralizing Antibodies against Hepatitis B Virus HBsAg with Distinct Epitopes Suppresses Escape Mutations. Authors: Wang, Q. / Michailidis, E. / Yu, Y. / Wang, Z. / Hurley, A.M. / Oren, D.A. / Mayer, C.T. / Gazumyan, A. / Liu, Z. / Zhou, Y. / Schoofs, T. / Yao, K.H. / Nieke, J.P. / Wu, J. / Jiang, Q. / ...Authors: Wang, Q. / Michailidis, E. / Yu, Y. / Wang, Z. / Hurley, A.M. / Oren, D.A. / Mayer, C.T. / Gazumyan, A. / Liu, Z. / Zhou, Y. / Schoofs, T. / Yao, K.H. / Nieke, J.P. / Wu, J. / Jiang, Q. / Zou, C. / Kabbani, M. / Quirk, C. / Oliveira, T. / Chhosphel, K. / Zhang, Q. / Schneider, W.M. / Jahan, C. / Ying, T. / Horowitz, J. / Caskey, M. / Jankovic, M. / Robbiani, D.F. / Wen, Y. / de Jong, Y.P. / Rice, C.M. / Nussenzweig, M.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 108.6 KB | Display | ![]() |
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PDB format | ![]() | 80 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 248 KB | Display | ![]() |
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Full document | ![]() | 247.9 KB | Display | |
Data in XML | ![]() | 928 B | Display | |
Data in CIF | ![]() | 7.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5gguS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 24024.889 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Antibody | Mass: 23310.910 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Protein/peptide | Mass: 1081.071 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.36 % / Description: plates |
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Crystal grow | Temperature: 300 K / Method: vapor diffusion, sitting drop / Details: Morpheus position E1 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 3, 2019 Details: Cryogenically-cooled single crystal Si(220) side bounce monochromator. | ||||||||||||||||||||||||||||||
Radiation | Monochromator: Cryogenically-cooled single crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 1.78→134.7 Å / Num. obs: 46432 / % possible obs: 99.5 % / Redundancy: 12.9 % / CC1/2: 0.999 / Rmerge(I) obs: 0.084 / Rpim(I) all: 0.025 / Rrim(I) all: 0.088 / Net I/σ(I): 17.2 / Num. measured all: 598818 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5GGU Resolution: 1.782→67.352 Å / SU ML: 0.21 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 24.48 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 119.32 Å2 / Biso mean: 39.4993 Å2 / Biso min: 20.84 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.782→67.352 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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