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- PDB-5tkj: Structure of vaccine-elicited diverse HIV-1 neutralizing antibody... -

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基本情報

登録情報
データベース: PDB / ID: 5tkj
タイトルStructure of vaccine-elicited diverse HIV-1 neutralizing antibody vFP1.01 in complex with HIV-1 fusion peptide residue 512-519
要素
  • HIV-1 fusion peptide residue 512-519
  • vFP1.01 chimeric mouse antibody heavy chain
  • vFP1.01 chimeric mouse antibody light chain
キーワードIMMUNE SYSTEM / Neutralizing / antibody / fusion-peptide / complex
機能・相同性
機能・相同性情報


Synthesis and processing of ENV and VPU / symbiont-mediated evasion of host immune response / positive regulation of establishment of T cell polarity / Alpha-defensins / Dectin-2 family / Binding and entry of HIV virion / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / host cell endosome membrane / actin filament organization ...Synthesis and processing of ENV and VPU / symbiont-mediated evasion of host immune response / positive regulation of establishment of T cell polarity / Alpha-defensins / Dectin-2 family / Binding and entry of HIV virion / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / host cell endosome membrane / actin filament organization / Assembly Of The HIV Virion / Budding and maturation of HIV virion / clathrin-dependent endocytosis of virus by host cell / viral protein processing / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / structural molecule activity / membrane
類似検索 - 分子機能
Envelope glycoprotein Gp160 / Retroviral envelope protein / Retroviral envelope protein GP41-like / Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120 / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
類似検索 - ドメイン・相同性
Envelope glycoprotein gp160
類似検索 - 構成要素
生物種Mus musculus (ハツカネズミ)
Human immunodeficiency virus 1 (ヒト免疫不全ウイルス)
手法X線回折 / シンクロトロン / 分子置換 / 解像度: 2.118 Å
データ登録者Xu, K. / Liu, K. / Kwong, P.D.
引用ジャーナル: Nat Med / : 2018
タイトル: Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1.
著者: Kai Xu / Priyamvada Acharya / Rui Kong / Cheng Cheng / Gwo-Yu Chuang / Kevin Liu / Mark K Louder / Sijy O'Dell / Reda Rawi / Mallika Sastry / Chen-Hsiang Shen / Baoshan Zhang / Tongqing Zhou ...著者: Kai Xu / Priyamvada Acharya / Rui Kong / Cheng Cheng / Gwo-Yu Chuang / Kevin Liu / Mark K Louder / Sijy O'Dell / Reda Rawi / Mallika Sastry / Chen-Hsiang Shen / Baoshan Zhang / Tongqing Zhou / Mangaiarkarasi Asokan / Robert T Bailer / Michael Chambers / Xuejun Chen / Chang W Choi / Venkata P Dandey / Nicole A Doria-Rose / Aliaksandr Druz / Edward T Eng / S Katie Farney / Kathryn E Foulds / Hui Geng / Ivelin S Georgiev / Jason Gorman / Kurt R Hill / Alexander J Jafari / Young D Kwon / Yen-Ting Lai / Thomas Lemmin / Krisha McKee / Tiffany Y Ohr / Li Ou / Dongjun Peng / Ariana P Rowshan / Zizhang Sheng / John-Paul Todd / Yaroslav Tsybovsky / Elise G Viox / Yiran Wang / Hui Wei / Yongping Yang / Amy F Zhou / Rui Chen / Lu Yang / Diana G Scorpio / Adrian B McDermott / Lawrence Shapiro / Bridget Carragher / Clinton S Potter / John R Mascola / Peter D Kwong /
要旨: A central goal of HIV-1 vaccine research is the elicitation of antibodies capable of neutralizing diverse primary isolates of HIV-1. Here we show that focusing the immune response to exposed N- ...A central goal of HIV-1 vaccine research is the elicitation of antibodies capable of neutralizing diverse primary isolates of HIV-1. Here we show that focusing the immune response to exposed N-terminal residues of the fusion peptide, a critical component of the viral entry machinery and the epitope of antibodies elicited by HIV-1 infection, through immunization with fusion peptide-coupled carriers and prefusion stabilized envelope trimers, induces cross-clade neutralizing responses. In mice, these immunogens elicited monoclonal antibodies capable of neutralizing up to 31% of a cross-clade panel of 208 HIV-1 strains. Crystal and cryoelectron microscopy structures of these antibodies revealed fusion peptide conformational diversity as a molecular explanation for the cross-clade neutralization. Immunization of guinea pigs and rhesus macaques induced similarly broad fusion peptide-directed neutralizing responses, suggesting translatability. The N terminus of the HIV-1 fusion peptide is thus a promising target of vaccine efforts aimed at eliciting broadly neutralizing antibodies.
履歴
登録2016年10月6日登録サイト: RCSB / 処理サイト: RCSB
改定 1.02018年4月4日Provider: repository / タイプ: Initial release
改定 1.12018年6月27日Group: Data collection / Database references / カテゴリ: citation / citation_author
Item: _citation.journal_abbrev / _citation.journal_id_CSD ..._citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
改定 1.22023年10月4日Group: Data collection / Database references / Refinement description
カテゴリ: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
改定 1.32024年11月20日Group: Structure summary
カテゴリ: pdbx_entry_details / pdbx_modification_feature

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構造の表示

構造ビューア分子:
MolmilJmol/JSmol

ダウンロードとリンク

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集合体

登録構造単位
A: vFP1.01 chimeric mouse antibody heavy chain
B: vFP1.01 chimeric mouse antibody light chain
C: HIV-1 fusion peptide residue 512-519
D: vFP1.01 chimeric mouse antibody heavy chain
E: vFP1.01 chimeric mouse antibody light chain
F: HIV-1 fusion peptide residue 512-519
G: vFP1.01 chimeric mouse antibody heavy chain
H: vFP1.01 chimeric mouse antibody light chain
I: HIV-1 fusion peptide residue 512-519
J: vFP1.01 chimeric mouse antibody heavy chain
K: vFP1.01 chimeric mouse antibody light chain
L: HIV-1 fusion peptide residue 512-519
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)195,82719
ポリマ-195,15512
非ポリマー6727
19,4381079
1
A: vFP1.01 chimeric mouse antibody heavy chain
B: vFP1.01 chimeric mouse antibody light chain
C: HIV-1 fusion peptide residue 512-519
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)48,9815
ポリマ-48,7893
非ポリマー1922
543
タイプ名称対称操作
identity operation1_555x,y,z1
2
D: vFP1.01 chimeric mouse antibody heavy chain
E: vFP1.01 chimeric mouse antibody light chain
F: HIV-1 fusion peptide residue 512-519
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)48,9815
ポリマ-48,7893
非ポリマー1922
543
タイプ名称対称操作
identity operation1_555x,y,z1
3
G: vFP1.01 chimeric mouse antibody heavy chain
H: vFP1.01 chimeric mouse antibody light chain
I: HIV-1 fusion peptide residue 512-519
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)48,9815
ポリマ-48,7893
非ポリマー1922
543
タイプ名称対称操作
identity operation1_555x,y,z1
4
J: vFP1.01 chimeric mouse antibody heavy chain
K: vFP1.01 chimeric mouse antibody light chain
L: HIV-1 fusion peptide residue 512-519
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)48,8854
ポリマ-48,7893
非ポリマー961
543
タイプ名称対称操作
identity operation1_555x,y,z1
単位格子
Length a, b, c (Å)76.159, 120.182, 226.182
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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要素

#1: 抗体
vFP1.01 chimeric mouse antibody heavy chain


分子量: 23927.898 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Mus musculus (ハツカネズミ) / 発現宿主: Homo sapiens (ヒト)
#2: 抗体
vFP1.01 chimeric mouse antibody light chain


分子量: 24127.859 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Mus musculus (ハツカネズミ) / 発現宿主: Homo sapiens (ヒト)
#3: タンパク質・ペプチド
HIV-1 fusion peptide residue 512-519


分子量: 732.868 Da / 分子数: 4 / 由来タイプ: 合成
由来: (合成) Human immunodeficiency virus 1 (ヒト免疫不全ウイルス)
参照: UniProt: P04578*PLUS
#4: 化合物
ChemComp-SO4 / SULFATE ION / 硫酸ジアニオン


分子量: 96.063 Da / 分子数: 7 / 由来タイプ: 合成 / : SO4
#5: 水 ChemComp-HOH / water


分子量: 18.015 Da / 分子数: 1079 / 由来タイプ: 天然 / : H2O
Has protein modificationY

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実験情報

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実験

実験手法: X線回折 / 使用した結晶の数: 1

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試料調製

結晶マシュー密度: 2.65 Å3/Da / 溶媒含有率: 53.62 %
結晶化温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.6 / 詳細: 0.2M AmSO4, 0.1M NaoAc pH 4.6, 25% PEG 4000

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データ収集

回折平均測定温度: 100 K
放射光源由来: シンクロトロン / サイト: APS / ビームライン: 22-ID / 波長: 1 Å
検出器タイプ: RAYONIX MX300-HS / 検出器: CCD / 日付: 2016年7月10日
放射プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長波長: 1 Å / 相対比: 1
反射解像度: 2.12→43.539 Å / Num. obs: 118381 / % possible obs: 99.8 % / 冗長度: 7.1 % / Biso Wilson estimate: 29.41 Å2 / Rmerge(I) obs: 0.093 / Rpim(I) all: 0.037 / Rrim(I) all: 0.1 / Χ2: 0.817 / Net I/av σ(I): 20.737 / Net I/σ(I): 5.4 / Num. measured all: 843512
反射 シェル
解像度 (Å)冗長度 (%)Rmerge(I) obsCC1/2Diffraction-ID% possible all
2.12-2.165.40.5170.899197.1
2.16-2.26.40.4890.93199.6
2.2-2.2470.4490.948199.8
2.24-2.2870.4020.959199.8
2.28-2.3370.3750.964199.8
2.33-2.397.10.3330.971199.9
2.39-2.4570.2950.9791100
2.45-2.517.10.2590.983199.9
2.51-2.597.10.2220.987199.9
2.59-2.677.10.1880.9891100
2.67-2.777.30.170.9911100
2.77-2.887.40.1390.9941100
2.88-3.017.50.120.9951100
3.01-3.177.50.1030.9961100
3.17-3.377.60.0820.9971100
3.37-3.627.50.0690.9981100
3.62-3.997.50.0640.9981100
3.99-4.577.50.0580.9981100
4.57-5.757.40.0480.9981100
5.75-5070.0460.999199.8

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解析

ソフトウェア
名称バージョン分類
PHENIX1.10_2155精密化
SERGUIデータ収集
HKL-2000データスケーリング
PDB_EXTRACT3.2データ抽出
HKL-2000データ削減
PHASER位相決定
精密化構造決定の手法: 分子置換
開始モデル: 3BKY
解像度: 2.118→43.539 Å / SU ML: 0.22 / 交差検証法: FREE R-VALUE / σ(F): 1.34 / 位相誤差: 29.6 / 立体化学のターゲット値: ML
Rfactor反射数%反射
Rfree0.2406 5929 5.02 %
Rwork0.1981 --
obs0.2003 118174 99.64 %
溶媒の処理減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL
精密化ステップサイクル: LAST / 解像度: 2.118→43.539 Å
タンパク質核酸リガンド溶媒全体
原子数13412 0 35 1079 14526
拘束条件
Refine-IDタイプDev ideal
X-RAY DIFFRACTIONf_bond_d0.00813788
X-RAY DIFFRACTIONf_angle_d1.00718790
X-RAY DIFFRACTIONf_dihedral_angle_d14.7018142
X-RAY DIFFRACTIONf_chiral_restr0.0622116
X-RAY DIFFRACTIONf_plane_restr0.0082368
LS精密化 シェル
解像度 (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.1185-2.14250.31381860.25083489X-RAY DIFFRACTION95
2.1425-2.16770.28282090.23253631X-RAY DIFFRACTION98
2.1677-2.19420.29441990.2383719X-RAY DIFFRACTION100
2.1942-2.22190.2832000.23533677X-RAY DIFFRACTION100
2.2219-2.25120.26961920.2263709X-RAY DIFFRACTION100
2.2512-2.2820.27572170.2183687X-RAY DIFFRACTION100
2.282-2.31460.24172110.22023708X-RAY DIFFRACTION100
2.3146-2.34920.28972010.23193686X-RAY DIFFRACTION100
2.3492-2.38590.2811970.22273744X-RAY DIFFRACTION100
2.3859-2.4250.24191870.21613689X-RAY DIFFRACTION100
2.425-2.46680.27042000.21943729X-RAY DIFFRACTION100
2.4668-2.51170.29031770.22483742X-RAY DIFFRACTION100
2.5117-2.560.30412030.21813723X-RAY DIFFRACTION100
2.56-2.61220.26521870.21613740X-RAY DIFFRACTION100
2.6122-2.6690.27241840.21623742X-RAY DIFFRACTION100
2.669-2.73110.27341980.21853759X-RAY DIFFRACTION100
2.7311-2.79940.29492070.21813699X-RAY DIFFRACTION100
2.7994-2.8750.23671840.21463741X-RAY DIFFRACTION100
2.875-2.95960.24631780.20953759X-RAY DIFFRACTION100
2.9596-3.05510.2782050.2033752X-RAY DIFFRACTION100
3.0551-3.16430.26251900.20683776X-RAY DIFFRACTION100
3.1643-3.29090.23551920.20473738X-RAY DIFFRACTION100
3.2909-3.44060.25092270.2023732X-RAY DIFFRACTION100
3.4406-3.6220.23632120.19753770X-RAY DIFFRACTION100
3.622-3.84880.21391740.18723807X-RAY DIFFRACTION100
3.8488-4.14570.21222160.17613765X-RAY DIFFRACTION100
4.1457-4.56250.18921910.1513816X-RAY DIFFRACTION100
4.5625-5.22180.18181910.14933855X-RAY DIFFRACTION100
5.2218-6.57520.21981900.18793876X-RAY DIFFRACTION100
6.5752-43.54780.212240.19863985X-RAY DIFFRACTION99
精密化 TLS

手法: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
10.81280.8853-0.63771.2614-0.30671.0123-0.00870.09670.26050.0875-0.00240.3612-0.2259-0.27490.00980.21490.0192-0.02450.2297-0.00080.36083.5222130.671666.3028
21.70990.10820.07020.00640.00450.0026-0.0035-0.15990.04210.2221-0.08870.36610.119-0.2410.07170.1807-0.04650.07970.3571-0.08110.3135-5.9662123.266273.9496
32.52640.9551-1.50122.2281-1.19454.12630.03560.1388-0.1274-0.09180.0132-0.04860.0512-0.0838-0.04810.15020.0062-0.02440.16240.01310.19163.289118.19862.0532
41.2333-0.11370.43581.7565-0.29332.24770.1082-0.1717-0.15790.3526-0.13280.05510.1077-0.1202-0.03140.2107-0.0587-0.05280.1910.03040.19762.3742114.015771.9968
52.5763-0.6116-0.39962.2460.42191.68090.1035-0.26650.00720.2956-0.09730.10620.1152-0.02060.00970.236-0.0185-0.04390.19980.01740.18915.7147120.558573.4022
62.01440.9628-0.18790.8706-0.20480.3013-0.0915-0.0950.1118-0.12350.00750.06520.07010.10370.06540.13940.0271-0.0140.1876-0.00920.20427.1107122.507664.3308
71.0180.33010.03522.6451-0.63110.89050.0035-0.08510.05210.0680.02080.20510.0058-0.26750.00050.1981-0.019-0.02480.24130.03280.2899-9.3186114.389466.6014
80.1698-0.310.26150.7279-0.47640.4328-0.02320.0250.0276-0.0462-0.0777-0.0148-0.18050.01570.05020.2259-0.0085-0.04770.15830.01580.224712.5277136.610557.9566
90.90650.45020.53510.86680.59040.9847-0.04090.13190.1279-0.32780.0313-0.0443-0.3090.16610.03970.3790.053-0.07030.26830.03680.272612.3445141.755537.1214
100.2825-0.12610.7140.0613-0.32521.82470.0631-0.01460.0725-0.1437-0.10040.0897-0.1435-0.14520.05610.38360.0833-0.08730.2247-0.02630.25354.2213142.373845.1518
110.7387-0.07080.13320.5851-0.0621.10360.01840.15090.1057-0.2461-0.1629-0.039-0.1913-0.07550.12150.33770.072-0.05940.23170.02880.2427.5836137.644838.0783
121.3152-0.05770.68311.4540.04025.36320.01820.1590.2038-0.2959-0.12150.0746-0.5313-0.0110.0780.51750.1081-0.08550.2436-0.00590.34536.4513148.728739.7034
132.1781-0.8380.84052.8362-1.74693.2777-0.02330.0112-0.1029-0.0483-0.1092-0.18280.01240.10510.11150.21330.00870.00160.2037-0.01190.2691-2.0904108.277345.9289
140.3350.3212-0.19341.4601-0.33811.83040.07880.0204-0.1216-0.0644-0.06880.16550.1843-0.23830.00970.1771-0.0303-0.06070.19010.01570.3064-8.5636108.250853.8642
150.25080.0286-0.28980.1549-0.14080.88010.06380.0482-0.086-0.04610.03130.04620.1246-0.0886-0.05790.2233-0.0002-0.08030.1515-0.00710.2387-0.0139113.001546.9431
161.98470.57340.41641.22870.28311.58520.1817-0.0388-0.0345-0.0255-0.1509-0.0277-0.17550.223-0.01890.22170.004-0.01090.21610.02020.228115.8053132.596333.7889
170.28170.0744-0.40771.002-0.02430.74870.09080.06050.0377-0.0479-0.0806-0.1202-0.06940.1411-0.00010.23460.0206-0.0410.22250.0110.246517.6826129.652832.5624
182.669-0.78430.46071.79971.20621.8-0.0861-0.6022-0.610.4590.01640.24550.5293-0.40780.08420.4676-0.1043-0.03420.31240.10330.344-4.8849104.253874.5692
190.37270.62240.13951.04690.16850.76580.07130.048-0.30070.0863-0.0355-0.26020.17730.18920.0150.27360.0545-0.070.2724-0.02160.427234.5713105.741966.3897
201.51960.12610.06720.1196-0.01620.1488-0.1098-0.11280.0210.1779-0.1155-0.3397-0.02320.23470.16350.2666-0.0048-0.11610.35490.05330.409344.0464113.22574.0414
211.92150.72581.37371.60920.88823.648-0.05660.1298-0.0631-0.1165-0.00790.0393-0.0606-0.0290.00110.19120.0109-0.03280.2491-0.04360.291834.818118.313562.0795
220.9836-0.1006-0.62111.74060.25712.19150.10480.068-0.08240.2062-0.0707-0.0029-0.09620.03370.00190.1782-0.0344-0.03830.1955-0.0050.241635.7221122.465172.0325
232.2751-0.62890.52041.6755-0.26321.14640.0905-0.1387-0.05040.3466-0.1075-0.1960.02240.04460.05020.22090.0053-0.05860.2330.03840.333632.3714115.917973.41
241.79890.70030.18050.84980.16650.50570.0276-0.0169-0.1927-0.05990.019-0.1049-0.0809-0.0561-0.01640.18090.0255-0.06170.2424-0.02150.293830.9762113.97864.3629
251.17810.73580.15682.84030.78660.88870.0272-0.0187-0.10440.1252-0.0609-0.1451-0.04850.28470.0350.19620.0004-0.0740.342-0.02670.402947.4192122.102366.6656
260.3760.05470.09850.53760.36480.85610.12950.1243-0.08930.0338-0.10580.01970.2903-0.0521-0.02230.33710.0747-0.0350.2302-0.05430.333825.716896.794445.2171
270.2308-0.0263-0.48390.06130.27791.86080.0925-0.05240.0588-0.1311-0.1825-0.05490.10720.13210.03550.32140.0961-0.04010.2009-0.01880.319733.871294.145545.1172
281.232-0.0349-0.67371.25830.49831.3890.15130.12470.0508-0.1213-0.1469-0.07240.08170.02750.03220.34070.1305-0.07340.2571-0.02570.295830.551798.912838.0307
290.76840.0307-0.44310.94080.28464.66150.00160.1095-0.2446-0.2112-0.15490.00250.41290.00180.11350.5160.1229-0.02210.2568-0.03450.370131.607587.726739.6046
301.7535-0.6909-0.44242.63011.59272.77860.0420.15590.06440.0262-0.11220.1798-0.0203-0.0730.05240.26330.0239-0.05670.32870.03460.32240.232128.221746.0203
310.54080.09820.47460.69920.29171.28140.06260.13290.0730.0366-0.0455-0.0360.01350.2459-0.02850.21640.03130.01380.2933-0.00750.334141.5708123.119950.0659
322.2950.9027-0.25370.5732-0.02430.10270.1408-0.14780.19490.0462-0.17380.11370.0320.01030.01050.32650.1007-0.02890.2771-0.06930.310424.5318109.523833.6291
330.1994-0.00320.28890.82980.34610.84550.09890.1775-0.0935-0.1576-0.13630.14890.2794-0.0070.07070.29420.0455-0.05880.2561-0.04020.327320.4001106.798732.3307
341.58520.2615-1.0050.9663-1.31182.0597-0.0238-0.34450.45810.3272-0.0014-0.0313-0.43430.27620.03090.4041-0.0699-0.03410.2736-0.00230.330442.5831132.448574.1216
350.86970.3467-0.57750.4265-0.7481.57080.3776-0.0811-0.66140.13660.0098-0.4750.19960.263-0.2930.3972-0.0191-0.24940.3474-0.01660.599535.6012106.57386.1904
360.3063-0.2389-0.13650.2518-0.02870.3380.25440.0447-0.78210.155-0.0963-0.41930.46180.0892-0.25570.45250.0364-0.22590.2710.00010.677430.695295.633878.5117
371.71980.20170.28070.2885-0.70812.12190.4375-0.1858-0.33890.3334-0.1533-0.10390.2076-0.1145-0.1110.3595-0.1237-0.19030.24670.09190.306621.3054102.107784.9283
382.16170.1539-0.15982.39340.20222.52120.30150.0884-0.55090.0329-0.1798-0.27610.1084-0.1874-0.23690.30050.0005-0.11090.26510.0110.348424.9667106.188579.3575
390.27610.5815-0.19981.545-0.24610.22830.348-0.1346-0.52580.0026-0.1083-0.21060.2514-0.0922-0.12360.5697-0.0876-0.27890.2540.09880.474125.085199.876487.2075
400.1484-0.1240.33760.1144-0.1751.68430.212-0.289-0.15350.26650.1131-0.30810.0160.3213-0.10520.4967-0.1887-0.2570.49380.03550.479242.1974117.6487105.6484
410.40010.0209-0.39040.0009-0.02060.3820.1913-0.2232-0.41060.26640.0384-0.20740.45090.3883-0.210.8414-0.0652-0.490.60620.04770.687246.1501107.5917111.1688
420.3416-0.268-0.03920.2146-0.03421.36760.1688-0.2241-0.26490.34490.0948-0.31390.340.2947-0.1060.7197-0.2546-0.42870.55760.13020.516242.8321113.4538115.8676
430.8048-0.1476-0.45010.2097-0.20510.70610.12130.0851-0.16180.10960.0953-0.1190.19380.5894-0.20760.649-0.0545-0.42440.6778-0.0660.778352.075114.2588109.6535
440.3020.00460.11560.0470.03780.080.2321-0.3085-0.15580.5013-0.2856-0.18410.2409-0.0883-0.24650.9964-0.4816-0.41650.58990.17270.555216.573890.501598.6387
450.0511-0.00990.10570.0023-0.02380.220.2975-0.3074-0.24910.4197-0.1826-0.20560.3508-0.1634-0.16320.9963-0.4996-0.37340.69350.19020.497718.633192.8586104.1893
460.055-0.1133-0.00390.24630.03080.32210.2737-0.4469-0.19750.2441-0.004-0.12090.4413-0.28540.31670.7612-0.5706-0.25150.8630.07240.417631.9137118.8617119.05
471.887-1.0476-0.86161.3865-0.00452.3133-0.03710.4579-0.5877-0.4654-0.09350.52690.6711-0.66740.16590.5737-0.1874-0.19270.46160.0310.423212.505491.411778.4627
481.63440.3078-0.09921.0452-0.130.94530.24-0.44190.4070.1517-0.23250.27770.1386-0.3655-0.02730.31-0.08850.01140.3309-0.06250.280710.5989133.074384.7754
490.2389-0.1235-0.10620.1657-0.0970.27360.1383-0.320.2120.16260.10440.2563-0.092-0.2392-0.04170.772-0.65860.27431.7222-0.1510.5865-7.7373123.3971113.3517
500.36910.0118-0.57420.8936-1.08352.1884-0.145-0.49010.24930.3395-0.1524-0.14050.1193-0.07870.18450.5248-0.2320.01220.6441-0.10560.361726.6644143.284100.1598
510.2794-0.1905-0.26210.4437-0.49231.74360.0472-0.53030.41030.3156-0.33140.0664-0.5325-0.3566-0.09920.5342-0.16680.0830.6712-0.26590.509518.2065145.5856101.5647
520.91570.1297-0.20510.7076-0.01540.279-0.0024-0.1828-0.0297-0.14130.13750.22110.1504-0.14430.05870.9014-0.7330.02731.68-0.07090.6023-0.2905115.7409118.5778
530.3247-0.05180.0330.2312-0.06950.51540.1275-0.2756-0.07360.04870.05280.0950.268-0.35520.23160.901-0.73390.12041.414-0.02090.42749.0498117.6393119.2638
543.3603-1.59121.51180.777-0.65860.8204-0.00110.54520.5997-0.4276-0.1389-0.5108-0.480.4520.17460.4288-0.16760.14580.3017-0.01390.300525.4695144.969578.2264
精密化 TLSグループ
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 17 )
2X-RAY DIFFRACTION2chain 'A' and (resid 18 through 32 )
3X-RAY DIFFRACTION3chain 'A' and (resid 33 through 43 )
4X-RAY DIFFRACTION4chain 'A' and (resid 44 through 59 )
5X-RAY DIFFRACTION5chain 'A' and (resid 60 through 82 )
6X-RAY DIFFRACTION6chain 'A' and (resid 83 through 95 )
7X-RAY DIFFRACTION7chain 'A' and (resid 96 through 106 )
8X-RAY DIFFRACTION8chain 'A' and (resid 107 through 119 )
9X-RAY DIFFRACTION9chain 'A' and (resid 120 through 145 )
10X-RAY DIFFRACTION10chain 'A' and (resid 146 through 157 )
11X-RAY DIFFRACTION11chain 'A' and (resid 158 through 189 )
12X-RAY DIFFRACTION12chain 'A' and (resid 190 through 213 )
13X-RAY DIFFRACTION13chain 'B' and (resid 1 through 18 )
14X-RAY DIFFRACTION14chain 'B' and (resid 19 through 90 )
15X-RAY DIFFRACTION15chain 'B' and (resid 91 through 113 )
16X-RAY DIFFRACTION16chain 'B' and (resid 114 through 150 )
17X-RAY DIFFRACTION17chain 'B' and (resid 151 through 213 )
18X-RAY DIFFRACTION18chain 'C' and (resid 512 through 519 )
19X-RAY DIFFRACTION19chain 'D' and (resid 1 through 17 )
20X-RAY DIFFRACTION20chain 'D' and (resid 18 through 32 )
21X-RAY DIFFRACTION21chain 'D' and (resid 33 through 43 )
22X-RAY DIFFRACTION22chain 'D' and (resid 44 through 59 )
23X-RAY DIFFRACTION23chain 'D' and (resid 60 through 82 )
24X-RAY DIFFRACTION24chain 'D' and (resid 83 through 95 )
25X-RAY DIFFRACTION25chain 'D' and (resid 96 through 106 )
26X-RAY DIFFRACTION26chain 'D' and (resid 107 through 145 )
27X-RAY DIFFRACTION27chain 'D' and (resid 146 through 157 )
28X-RAY DIFFRACTION28chain 'D' and (resid 158 through 189 )
29X-RAY DIFFRACTION29chain 'D' and (resid 190 through 213 )
30X-RAY DIFFRACTION30chain 'E' and (resid 1 through 18 )
31X-RAY DIFFRACTION31chain 'E' and (resid 19 through 128 )
32X-RAY DIFFRACTION32chain 'E' and (resid 129 through 150 )
33X-RAY DIFFRACTION33chain 'E' and (resid 151 through 214 )
34X-RAY DIFFRACTION34chain 'F' and (resid 512 through 519 )
35X-RAY DIFFRACTION35chain 'G' and (resid 1 through 17 )
36X-RAY DIFFRACTION36chain 'G' and (resid 18 through 32 )
37X-RAY DIFFRACTION37chain 'G' and (resid 33 through 59 )
38X-RAY DIFFRACTION38chain 'G' and (resid 60 through 82 )
39X-RAY DIFFRACTION39chain 'G' and (resid 83 through 106 )
40X-RAY DIFFRACTION40chain 'G' and (resid 107 through 145 )
41X-RAY DIFFRACTION41chain 'G' and (resid 146 through 165 )
42X-RAY DIFFRACTION42chain 'G' and (resid 166 through 194 )
43X-RAY DIFFRACTION43chain 'G' and (resid 195 through 213 )
44X-RAY DIFFRACTION44chain 'H' and (resid 1 through 61 )
45X-RAY DIFFRACTION45chain 'H' and (resid 62 through 113 )
46X-RAY DIFFRACTION46chain 'H' and (resid 114 through 211 )
47X-RAY DIFFRACTION47chain 'I' and (resid 512 through 519 )
48X-RAY DIFFRACTION48chain 'J' and (resid 1 through 119 )
49X-RAY DIFFRACTION49chain 'J' and (resid 120 through 213 )
50X-RAY DIFFRACTION50chain 'K' and (resid 1 through 32 )
51X-RAY DIFFRACTION51chain 'K' and (resid 33 through 113 )
52X-RAY DIFFRACTION52chain 'K' and (resid 114 through 139 )
53X-RAY DIFFRACTION53chain 'K' and (resid 140 through 211 )
54X-RAY DIFFRACTION54chain 'L' and (resid 512 through 519 )

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月:コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか? / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例:em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る