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Open data
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Basic information
Entry | Database: PDB / ID: 4uin | ||||||
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Title | crystal structure of quinine-dependent Fab 314.3 with quinine | ||||||
![]() | (FAB 314.3) x 2 | ||||||
![]() | IMMUNE SYSTEM / QUININE-DEPENDENT / MOUSE MAB | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / Quinine![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhu, J. / Zhu, J. / Bougie, D.W. / Aster, R.H. / Springer, T.A. | ||||||
![]() | ![]() Title: Structural Basis for Quinine-Dependent Antibody Binding to Platelet Integrin Alphaiib Beta3 Authors: Zhu, J. / Zhu, J. / Bougie, D.W. / Aster, R.H. / Springer, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 249.7 KB | Display | ![]() |
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PDB format | ![]() | 206.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 814.3 KB | Display | ![]() |
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Full document | ![]() | 817.1 KB | Display | |
Data in XML | ![]() | 17.8 KB | Display | |
Data in CIF | ![]() | 24.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4uikSC ![]() 4uilC ![]() 4uimC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 24257.135 Da / Num. of mol.: 1 / Fragment: HEAVY CHAIN, RESIDUES 1-225 / Source method: isolated from a natural source / Details: ASCITES FLUID / Source: (natural) ![]() ![]() |
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#2: Antibody | Mass: 23590.900 Da / Num. of mol.: 1 / Fragment: LIGHT CHAIN, RESIDUES 1-214 / Source method: isolated from a natural source / Details: ASCITES FLUID / Source: (natural) ![]() ![]() |
#3: Chemical | ChemComp-QI9 / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.04 % / Description: NONE |
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Crystal grow | pH: 7.5 / Details: 25% PEG 1500, PH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 10, 2012 / Details: DOUBLE MIRROR |
Radiation | Monochromator: SI CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.03321 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→50 Å / Num. obs: 14747 / % possible obs: 99.3 % / Observed criterion σ(I): 0 / Redundancy: 3.7 % / Biso Wilson estimate: 26.11 Å2 / Rmerge(I) obs: 0.13 / Net I/σ(I): 9.6 |
Reflection shell | Resolution: 2.5→2.59 Å / Redundancy: 3 % / Rmerge(I) obs: 0.76 / Mean I/σ(I) obs: 1.5 / % possible all: 95.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4UIK Resolution: 2.5→43.771 Å / SU ML: 0.32 / σ(F): 1.99 / Phase error: 25.39 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.7 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→43.771 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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