#256 - Apr 2021 SARS-CoV-2 Spike and Antibodies similarity (1)
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Assembly
Deposited unit
H: Heavy chain of bovine anti-RSV B13 Fab L: Light chain of bovine anti-RSV Fab B13 A: Heavy chain of bovine anti-RSV B13 Fab B: Light chain of bovine anti-RSV Fab B13 C: Heavy chain of bovine anti-RSV B13 Fab D: Light chain of bovine anti-RSV Fab B13 E: Heavy chain of bovine anti-RSV B13 Fab F: Light chain of bovine anti-RSV Fab B13 G: Heavy chain of bovine anti-RSV B13 Fab I: Light chain of bovine anti-RSV Fab B13 J: Heavy chain of bovine anti-RSV B13 Fab K: Light chain of bovine anti-RSV Fab B13 hetero molecules
Mass: 25837.941 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Cell line (production host): Expi293 / Production host: Homo sapiens (human)
#2: Antibody
Lightchainofbovineanti-RSVFabB13
Mass: 22635.068 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Production host: Homo sapiens (human)
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.9282 Å / Relative weight: 1
Reflection
Resolution: 2.12→115.8 Å / Num. obs: 148202 / % possible obs: 99.2 % / Redundancy: 6.8 % / CC1/2: 0.99 / Rmerge(I) obs: 0.128 / Net I/σ(I): 19.4
Reflection shell
Resolution: 2.12→2.16 Å / Redundancy: 6 % / Num. unique obs: 7273 / CC1/2: 0.58 / % possible all: 97.6
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0238
refinement
xia2
datareduction
xia2
datascaling
PHASER
phasing
Refinement
Resolution: 2.12→87.07 Å / Cor.coef. Fo:Fc: 0.933 / Cor.coef. Fo:Fc free: 0.917 / SU B: 16.421 / SU ML: 0.205 / Cross valid method: THROUGHOUT / ESU R: 0.309 / ESU R Free: 0.223 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.267
7327
5 %
RANDOM
Rwork
0.23529
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obs
0.23687
140486
98.98 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å