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- PDB-4qum: Crystal structure of PTPN3 (PTPH1) in complex with a dually phosp... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4qum | ||||||
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Title | Crystal structure of PTPN3 (PTPH1) in complex with a dually phosphorylated MAPK12 peptide | ||||||
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![]() | HYDROLASE / Alpha Beta | ||||||
Function / homology | ![]() positive regulation of peptidase activity / regulation of membrane depolarization during action potential / negative regulation of membrane protein ectodomain proteolysis / regulation of sodium ion transmembrane transporter activity / myoblast differentiation / Activation of PPARGC1A (PGC-1alpha) by phosphorylation / muscle organ development / DSCAM interactions / positive regulation of muscle cell differentiation / Myogenesis ...positive regulation of peptidase activity / regulation of membrane depolarization during action potential / negative regulation of membrane protein ectodomain proteolysis / regulation of sodium ion transmembrane transporter activity / myoblast differentiation / Activation of PPARGC1A (PGC-1alpha) by phosphorylation / muscle organ development / DSCAM interactions / positive regulation of muscle cell differentiation / Myogenesis / negative regulation of epidermal growth factor receptor signaling pathway / negative regulation of cell cycle / negative regulation of mitotic cell cycle / MAP kinase activity / sodium channel regulator activity / mitogen-activated protein kinase / signal transduction in response to DNA damage / p38MAPK events / cytoskeletal protein binding / phosphotyrosine residue binding / protein dephosphorylation / protein-tyrosine-phosphatase / protein tyrosine phosphatase activity / liver regeneration / EGFR downregulation / NOD1/2 Signaling Pathway / cytoplasmic side of plasma membrane / VEGFA-VEGFR2 Pathway / Negative regulation of MAPK pathway / MAPK cascade / ATPase binding / peptidyl-serine phosphorylation / cytoskeleton / regulation of cell cycle / intracellular signal transduction / cell cycle / protein serine kinase activity / protein serine/threonine kinase activity / magnesium ion binding / signal transduction / mitochondrion / nucleoplasm / ATP binding / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chen, K.E. / Meng, T.C. / Wang, A.H.J. | ||||||
![]() | ![]() Title: Reciprocal allosteric regulation of p38 gamma and PTPN3 involves a PDZ domain-modulated complex formation. Authors: Chen, K.E. / Lin, S.Y. / Wu, M.J. / Ho, M.R. / Santhanam, A. / Chou, C.C. / Meng, T.C. / Wang, A.H.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 73.2 KB | Display | ![]() |
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PDB format | ![]() | 52.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 434.9 KB | Display | ![]() |
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Full document | ![]() | 436.6 KB | Display | |
Data in XML | ![]() | 12.8 KB | Display | |
Data in CIF | ![]() | 16.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4qunC ![]() 2b49S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34887.664 Da / Num. of mol.: 1 / Fragment: Catalytic domain (UNP RESIDUES 628-909) / Mutation: D811A, C842S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1244.252 Da / Num. of mol.: 1 / Fragment: Activation loop (UNP RESIDUES 182-190) / Mutation: W190R / Source method: obtained synthetically / Source: (synth.) ![]() References: UniProt: P53778, mitogen-activated protein kinase |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.71 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.1M Tris-HCl, 30% PEG 8000, 5% glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: Mar 6, 2013 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.51→50 Å / Num. all: 12505 / Num. obs: 12505 / % possible obs: 98 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.7 % / Biso Wilson estimate: 41.1 Å2 / Rmerge(I) obs: 0.122 / Net I/σ(I): 10.79 |
Reflection shell | Resolution: 2.51→2.6 Å / Redundancy: 4 % / Rmerge(I) obs: 0.521 / Mean I/σ(I) obs: 2.35 / Num. unique all: 1182 / % possible all: 95.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB Entry 2B49 Resolution: 2.516→31.325 Å / SU ML: 0.34 / σ(F): 1.34 / Phase error: 25.22 / Stereochemistry target values: MLHL
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.516→31.325 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 4
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