- PDB-4k1g: Structure of E. coli Nfo(Endo IV)-H69A mutant bound to a cleaved ... -
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IDまたはキーワード:
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基本情報
登録情報
データベース: PDB / ID: 4k1g
タイトル
Structure of E. coli Nfo(Endo IV)-H69A mutant bound to a cleaved DNA duplex containing a alphadA:T basepair
要素
DNA (5'-D(*CP*GP*TP*CP*GP*TP*CP*GP*TP*GP*GP*AP*CP*GP*C)-3')
DNA (5'-D(*GP*CP*GP*TP*CP*C)-3')
DNA (5'-D(P*AP*CP*GP*AP*CP*GP*AP*CP*G)-3')
Endonuclease 4
キーワード
HYDROLASE/DNA / DNA endonuclease IV / HYDROLASE-DNA complex
機能・相同性
機能・相同性情報
deoxyribonuclease IV / deoxyribonuclease IV (phage-T4-induced) activity / phosphoric diester hydrolase activity / 3'-5'-DNA exonuclease activity / phosphatase activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / base-excision repair / endonuclease activity / DNA repair / DNA binding ...deoxyribonuclease IV / deoxyribonuclease IV (phage-T4-induced) activity / phosphoric diester hydrolase activity / 3'-5'-DNA exonuclease activity / phosphatase activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / base-excision repair / endonuclease activity / DNA repair / DNA binding / zinc ion binding / cytosol 類似検索 - 分子機能
AP endonucleases family 2 signature 1. / AP endonucleases family 2 signature 2. / AP endonuclease 2, zinc binding site / AP endonucleases family 2 signature 3. / AP endonucleases family 2 profile. / AP endonuclease 2 / AP endonuclease family 2 / Divalent-metal-dependent TIM barrel enzymes / Xylose isomerase-like, TIM barrel domain / Xylose isomerase-like TIM barrel ...AP endonucleases family 2 signature 1. / AP endonucleases family 2 signature 2. / AP endonuclease 2, zinc binding site / AP endonucleases family 2 signature 3. / AP endonucleases family 2 profile. / AP endonuclease 2 / AP endonuclease family 2 / Divalent-metal-dependent TIM barrel enzymes / Xylose isomerase-like, TIM barrel domain / Xylose isomerase-like TIM barrel / Xylose isomerase-like superfamily / TIM Barrel / Alpha-Beta Barrel / Alpha Beta 類似検索 - ドメイン・相同性
DI(HYDROXYETHYL)ETHER / DNA / DNA (> 10) / Endonuclease 4 類似検索 - 構成要素
A: Endonuclease 4 B: Endonuclease 4 E: DNA (5'-D(*GP*CP*GP*TP*CP*C)-3') F: DNA (5'-D(*CP*GP*TP*CP*GP*TP*CP*GP*TP*GP*GP*AP*CP*GP*C)-3') H: DNA (5'-D(P*AP*CP*GP*AP*CP*GP*AP*CP*G)-3') M: DNA (5'-D(*GP*CP*GP*TP*CP*C)-3') N: DNA (5'-D(*CP*GP*TP*CP*GP*TP*CP*GP*TP*GP*GP*AP*CP*GP*C)-3') O: DNA (5'-D(P*AP*CP*GP*AP*CP*GP*AP*CP*G)-3') ヘテロ分子
A: Endonuclease 4 E: DNA (5'-D(*GP*CP*GP*TP*CP*C)-3') F: DNA (5'-D(*CP*GP*TP*CP*GP*TP*CP*GP*TP*GP*GP*AP*CP*GP*C)-3') H: DNA (5'-D(P*AP*CP*GP*AP*CP*GP*AP*CP*G)-3') ヘテロ分子
B: Endonuclease 4 M: DNA (5'-D(*GP*CP*GP*TP*CP*C)-3') N: DNA (5'-D(*CP*GP*TP*CP*GP*TP*CP*GP*TP*GP*GP*AP*CP*GP*C)-3') O: DNA (5'-D(P*AP*CP*GP*AP*CP*GP*AP*CP*G)-3') ヘテロ分子
AUTHORS STATE THAT THE QUATERNARY STRUCTURE IS A PROTEIN MONOMER-DOUBLE STRANDED DNA COMPLEX. ONE OF THE DNA STRANDS IS CLEAVED BETWEEN THE BASE 306 AND THE BASE 307.
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要素
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タンパク質 , 1種, 2分子 AB
#1: タンパク質
Endonuclease4 / Endodeoxyribonuclease IV / Endonuclease IV
CHAINS E AND H, AND CHAINS M AND O ARE FORM THE SAME DNA STRAND WHICH IS CLEAVED BETWEEN THE BASE ...CHAINS E AND H, AND CHAINS M AND O ARE FORM THE SAME DNA STRAND WHICH IS CLEAVED BETWEEN THE BASE 306 AND THE BASE 307.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.79 Å3/Da / 溶媒含有率: 55.94 %
結晶化
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8 詳細: PEG 4000, Tris HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K