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Open data
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Basic information
Entry | Database: PDB / ID: 4avp | ||||||
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Title | Crystal structure of the DNA-binding domain of human ETV1. | ||||||
![]() | ETS TRANSLOCATION VARIANT 1 | ||||||
![]() | TRANSCRIPTION / TRANSCRIPTIONAL ACTIVATION AND REPRESSION / DNA BINDING PROTEIN / E TWENTY-SIX / ERWING SARCOMA / PROSTATE CANCER / MELANOMA / GASTROINTESTINAL STROMAL TUMOUR | ||||||
Function / homology | ![]() peripheral nervous system neuron development / mechanosensory behavior / muscle organ development / axon guidance / sequence-specific double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / transcription by RNA polymerase II / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity ...peripheral nervous system neuron development / mechanosensory behavior / muscle organ development / axon guidance / sequence-specific double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / transcription by RNA polymerase II / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / chromatin / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Allerston, C.K. / Cooper, C.D.O. / Krojer, T. / Chaikuad, A. / Filippakopoulos, P. / Canning, P. / Arrowsmith, C.H. / Edwards, A. / Bountra, C. / von Delft, F. / Gileadi, O. | ||||||
![]() | ![]() Title: Structures of the Ets Domains of Transcription Factors Etv1, Etv4, Etv5 and Fev: Determinants of DNA Binding and Redox Regulation by Disulfide Bond Formation. Authors: Cooper, C.D.O. / Newman, J.A. / Aitkenhead, H. / Allerston, C.K. / Gileadi, O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 92.4 KB | Display | ![]() |
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PDB format | ![]() | 69.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 463.9 KB | Display | ![]() |
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Full document | ![]() | 465.4 KB | Display | |
Data in XML | ![]() | 17.7 KB | Display | |
Data in CIF | ![]() | 24.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2yprC ![]() 3zp5C ![]() 4bncC ![]() 4co8C ![]() 4unoC ![]() 4uuvC ![]() 1gvjS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given) |
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Components
#1: Protein | Mass: 12437.209 Da / Num. of mol.: 4 / Fragment: DNA-BINDING DOMAIN, RESIDUES 326-429 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Compound details | ENGINEERED RESIDUE IN CHAIN A, TYR 329 TO SER ENGINEERED RESIDUE IN CHAIN B, TYR 329 TO SER ...ENGINEERED | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.72 Å3/Da / Density % sol: 28.55 % / Description: NONE |
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Crystal grow | pH: 7.5 / Details: 2.5M SODIUM FORMATE, pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH MARMOSAIC 300 / Detector: CCD / Date: Apr 29, 2012 / Details: MIRRORS |
Radiation | Monochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.82→53.94 Å / Num. obs: 27819 / % possible obs: 97.4 % / Observed criterion σ(I): 2 / Redundancy: 2.3 % / Biso Wilson estimate: 22.04 Å2 / Rmerge(I) obs: 0.054 / Rpim(I) all: 0.062 / Net I/σ(I): 10.4 |
Reflection shell | Resolution: 1.82→1.92 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.33 / Mean I/σ(I) obs: 2.3 / Rpim(I) all: 0.36 / % possible all: 96.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1GVJ Resolution: 1.82→53.94 Å / Cor.coef. Fo:Fc: 0.9259 / Cor.coef. Fo:Fc free: 0.8979 / SU R Cruickshank DPI: 0.19 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.187 / SU Rfree Blow DPI: 0.159 / SU Rfree Cruickshank DPI: 0.161 Details: IDEAL-DIST CONTACT TERM CONTACT SETUP. ALL ATOMS HAVE CCP4 ATOM TYPE FROM LIBRARY
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Displacement parameters | Biso mean: 23.4 Å2
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Refinement step | Cycle: LAST / Resolution: 1.82→53.94 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.82→1.89 Å / Total num. of bins used: 14
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