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Open data
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Basic information
Entry | Database: PDB / ID: 1i0x | ||||||
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Title | RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL) | ||||||
![]() | GUANYL-SPECIFIC RIBONUCLEASE T1 | ||||||
![]() | HYDROLASE / Ribonuclease / RNase / metal binding / stability / 2'GMP | ||||||
Function / homology | ![]() hyphal tip / ribonuclease T1 activity / ribonuclease T1 / cell septum / endonuclease activity / lyase activity / RNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | De Swarte, J. / De Vos, S. / Langhorst, U. / Steyaert, J. / Loris, R. | ||||||
![]() | ![]() Title: The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution. Authors: Deswarte, J. / De Vos, S. / Langhorst, U. / Steyaert, J. / Loris, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 102.3 KB | Display | ![]() |
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PDB format | ![]() | 83 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2 MB | Display | ![]() |
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Full document | ![]() | 2 MB | Display | |
Data in XML | ![]() | 24.7 KB | Display | |
Data in CIF | ![]() | 35.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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5 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11094.694 Da / Num. of mol.: 4 / Mutation: Q25K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-2GP / #3: Chemical | ChemComp-CA / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.96 Å3/Da / Density % sol: 37.29 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.2 Details: MPD, acetate, calcium chloride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 11, 1998 / Details: graphite monochromator |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→15 Å / Num. all: 40949 / Num. obs: 40949 / % possible obs: 95.7 % / Observed criterion σ(F): -6 / Observed criterion σ(I): -3 / Redundancy: 5.55 % / Biso Wilson estimate: 13.67 Å2 / Rmerge(I) obs: 0.1 / Net I/σ(I): 12.81 |
Reflection shell | Resolution: 1.65→1.7 Å / Redundancy: 5.36 % / Rmerge(I) obs: 0.534 / Mean I/σ(I) obs: 2.72 / Num. unique all: 3920 / % possible all: 92.4 |
Reflection | *PLUS Num. obs: 41132 / Num. measured all: 228296 / Rmerge(I) obs: 0.1 |
Reflection shell | *PLUS % possible obs: 92.4 % / Num. unique obs: 3920 / Num. measured obs: 20995 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.65→15 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 15 Å / σ(F): 0 / Rfactor obs: 0.1903 / Rfactor Rfree: 0.216 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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