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- PDB-3ztj: Structure of influenza A neutralizing antibody selected from cult... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3ztj | |||||||||
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Title | Structure of influenza A neutralizing antibody selected from cultures of single human plasma cells in complex with human H3 Influenza haemagglutinin. | |||||||||
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![]() | IMMUNE SYSTEM/VIRAL PROTEIN / IMMUNE SYSTEM-VIRAL PROTEIN COMPLEX / X31 / MONOCLONAL ANTIBODY | |||||||||
Function / homology | ![]() viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Voss, J.E. / Vachieri, S.G. / Gamblin, S.J. / Collins, P.J. / Haire, L.F. / Skehel, J.J. | |||||||||
![]() | ![]() Title: A Neutralizing Antibody Selected from Plasma Cells that Binds to Group 1 and Group 2 Influenza a Hemagglutinins. Authors: Corti, D. / Voss, J.E. / Gamblin, S.J. / Codoni, G. / Macagno, A. / Jarrossay, D. / Vachieri, S.G. / Pinna, D. / Minola, A. / Vanzetta, F. / Silacci, C. / Fernandez-Rodriguez, B.M. / Agatic, ...Authors: Corti, D. / Voss, J.E. / Gamblin, S.J. / Codoni, G. / Macagno, A. / Jarrossay, D. / Vachieri, S.G. / Pinna, D. / Minola, A. / Vanzetta, F. / Silacci, C. / Fernandez-Rodriguez, B.M. / Agatic, G. / Bianchi, S. / Giacchetto-Sasselli, I. / Calder, L. / Sallusto, F. / Collins, P.J. / Haire, L.F. / Temperton, N. / Langedijk, J.P.M. / Skehel, J.J. / Lanzavecchia, A. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1 MB | Display | ![]() |
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PDB format | ![]() | 877.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 4.3 MB | Display | ![]() |
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Full document | ![]() | 4.4 MB | Display | |
Data in XML | ![]() | 98.4 KB | Display | |
Data in CIF | ![]() | 133.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
-HEMAGGLUTININ ... , 2 types, 6 molecules ACEBDF
#1: Protein | Mass: 36222.652 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Protein | Mass: 20212.350 Da / Num. of mol.: 3 / Fragment: RESIDUES 346-520 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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-Antibody , 2 types, 6 molecules GIKHJL
#3: Antibody | Mass: 24557.555 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Antibody | Mass: 24105.758 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Sugars , 3 types, 15 molecules ![](data/chem/img/NAG.gif)
#5: Polysaccharide | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #6: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #7: Sugar | ChemComp-NAG / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.62 Å3/Da / Density % sol: 75 % / Description: NONE |
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-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 3.4→143.4 Å / Num. obs: 97028 / % possible obs: 96.1 % / Observed criterion σ(I): 2 / Redundancy: 2.9 % / Rmerge(I) obs: 0.12 / Net I/σ(I): 14 |
Reflection shell | Resolution: 3.41→3.55 Å / Redundancy: 2.4 % / Rmerge(I) obs: 0.6 / Mean I/σ(I) obs: 1.6 / % possible all: 93.2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 152.486 Å2
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Refinement step | Cycle: LAST / Resolution: 3.41→143.42 Å
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