+Open data
-Basic information
Entry | Database: PDB / ID: 2viu | |||||||||
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Title | INFLUENZA VIRUS HEMAGGLUTININ | |||||||||
Components | (HEMAGGLUTININ) x 2 | |||||||||
Keywords | HEMAGGLUTININ / ENVELOPE PROTEIN / GLYCOPROTEIN | |||||||||
Function / homology | Function and homology information viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / membrane Similarity search - Function | |||||||||
Biological species | Influenza A virus unidentified influenza virus | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.5 Å | |||||||||
Authors | Bizebard, T. / Fleury, D. / Gigant, B. / Wharton, S.A. / Skehel, J.J. / Knossow, M. | |||||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1998 Title: Antigen distortion allows influenza virus to escape neutralization. Authors: Fleury, D. / Wharton, S.A. / Skehel, J.J. / Knossow, M. / Bizebard, T. #1: Journal: Nature / Year: 1995 Title: Structure of Influenza Virus Haemagglutinin Complexed with a Neutralizing Antibody Authors: Bizebard, T. / Gigant, B. / Rigolet, P. / Rasmussen, B. / Diat, O. / Bosecke, P. / Wharton, S.A. / Skehel, J.J. / Knossow, M. #2: Journal: Acta Crystallogr.,Sect.D / Year: 1994 Title: Refined Three-Dimensional Structure of the Fab Fragment of a Murine Iggl, Authors: Bizebard, T. / Daniels, R. / Kahn, R. / Golinelli-Pimpaneau, B. / Skehel, J.J. / Knossow, M. #3: Journal: Nature / Year: 1981 Title: Structure of the Haemagglutinin Membrane Glycoprotein of Influenza Virus at 3 A Resolution Authors: Wilson, I.A. / Skehel, J.J. / Wiley, D.C. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2viu.cif.gz | 111.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2viu.ent.gz | 89.9 KB | Display | PDB format |
PDBx/mmJSON format | 2viu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vi/2viu ftp://data.pdbj.org/pub/pdb/validation_reports/vi/2viu | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36077.512 Da / Num. of mol.: 1 / Mutation: CHAIN A, T131I Source method: isolated from a genetically manipulated source Details: BROMELAIN DIGESTED / Source: (gene. exp.) Influenza A virus (A/X-31(H3N2)) / Genus: Influenzavirus A / Species: Influenza A virus / Strain: X31 Description: A REASSORTANT INFLUENZA STRAIN CONTAINING A/AICHI/68 (H3N2) HEMAGGLUTININ References: UniProt: P03437 | ||
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#2: Protein | Mass: 20212.350 Da / Num. of mol.: 1 / Mutation: CHAIN A, T131I Source method: isolated from a genetically manipulated source Details: BROMELAIN DIGESTED / Source: (gene. exp.) unidentified influenza virus / Strain: X31 Description: A REASSORTANT INFLUENZA STRAIN CONTAINING A/AICHI/68 (H3N2) HEMAGGLUTININ References: UniProt: P03437 | ||
#3: Polysaccharide | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source | ||
#4: Sugar | ChemComp-NAG / #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 4.46 Å3/Da / Density % sol: 66 % | ||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: LURE / Beamline: DW32 / Wavelength: 0.97 |
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Apr 8, 1997 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 2.45→20 Å / Num. obs: 36234 / % possible obs: 99.9 % / Redundancy: 4.5 % / Rmerge(I) obs: 0.055 |
Reflection | *PLUS Num. measured all: 163053 |
-Processing
Software |
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Refinement | Resolution: 2.5→9 Å / σ(F): 0 Details: THERE IS ONE MONOMER OF THE TRIMERIC HEMAGGLUTININ MOLECULE IN THE ASYMMETRIC UNIT. THE MONOMER OF HEMAGGLUTININ CONSISTS OF TWO CHAINS, IDENTIFIED AS HA1 AND HA2 BY THE THE DEPOSITORS. ...Details: THERE IS ONE MONOMER OF THE TRIMERIC HEMAGGLUTININ MOLECULE IN THE ASYMMETRIC UNIT. THE MONOMER OF HEMAGGLUTININ CONSISTS OF TWO CHAINS, IDENTIFIED AS HA1 AND HA2 BY THE THE DEPOSITORS. CHAINS HA1 AND HA2 HAVE BEEN ASSIGNED CHAIN IDENTIFIERS *A* AND *B*, RESPECTIVELY.
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Refinement step | Cycle: LAST / Resolution: 2.5→9 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.84 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 8 Å / Rfactor obs: 0.213 / Rfactor Rfree: 0.239 / Rfactor Rwork: 0.21 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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