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- PDB-2vis: INFLUENZA VIRUS HEMAGGLUTININ, (ESCAPE) MUTANT WITH THR 131 REPLA... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2vis | ||||||
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Title | INFLUENZA VIRUS HEMAGGLUTININ, (ESCAPE) MUTANT WITH THR 131 REPLACED BY ILE, COMPLEXED WITH A NEUTRALIZING ANTIBODY | ||||||
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![]() | COMPLEX (HEMAGGLUTININ/IMMUNOGLOBULIN) / COMPLEX (HEMAGGLUTININ-IMMUNOGLOBULIN) / GLYCOPROTEIN / COMPLEX (HEMAGGLUTININ-IMMUNOGLOBULIN) complex | ||||||
Function / homology | ![]() immunoglobulin complex, circulating / immunoglobulin receptor binding / viral budding from plasma membrane / complement activation, classical pathway / antigen binding / antibacterial humoral response / clathrin-dependent endocytosis of virus by host cell / blood microparticle / host cell surface receptor binding / fusion of virus membrane with host plasma membrane ...immunoglobulin complex, circulating / immunoglobulin receptor binding / viral budding from plasma membrane / complement activation, classical pathway / antigen binding / antibacterial humoral response / clathrin-dependent endocytosis of virus by host cell / blood microparticle / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / extracellular exosome / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Bizebard, T. / Fleury, D. / Gigant, B. / Wharton, S.A. / Skehel, J.J. / Knossow, M. | ||||||
![]() | ![]() Title: Antigen distortion allows influenza virus to escape neutralization. Authors: Fleury, D. / Wharton, S.A. / Skehel, J.J. / Knossow, M. / Bizebard, T. #1: ![]() Title: Structure of Influenza Virus Haemagglutinin Complexed with a Neutralizing Antibody Authors: Bizebard, T. / Gigant, B. / Rigolet, P. / Rasmussen, B. / Diat, O. / Bosecke, P. / Wharton, S.A. / Skehel, J.J. / Knossow, M. #2: ![]() Title: Refined Three-Dimensional Structure of the Fab Fragment of a Murine Iggl, Authors: Bizebard, T. / Daniels, R. / Kahn, R. / Golinelli-Pimpaneau, B. / Skehel, J.J. / Knossow, M. #3: ![]() Title: Structure of the Haemagglutinin Membrane Glycoprotein of Influenza Virus at 3 A Resolution Authors: Wilson, I.A. / Skehel, J.J. / Wiley, D.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 144.6 KB | Display | ![]() |
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PDB format | ![]() | 117.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 410.2 KB | Display | ![]() |
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Full document | ![]() | 434.4 KB | Display | |
Data in XML | ![]() | 18.1 KB | Display | |
Data in CIF | ![]() | 27 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 22596.021 Da / Num. of mol.: 1 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||||
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#2: Antibody | Mass: 23777.783 Da / Num. of mol.: 1 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||||
#3: Protein | Mass: 31175.979 Da / Num. of mol.: 1 Fragment: PROTEOLYTIC FRAGMENT "HA TOP" CONTAINING HA1 RESIDUES 28 - 328 Mutation: T131I Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Description: A REASSORTANT INFLUENZA STRAIN CONTAINING A/AICHI/68 (H3N2) HEMAGGLUTININ References: UniProt: P03437 | ||||
#4: Chemical | #5: Sugar | ChemComp-NAG / | Compound details | THE TWO MOLECULES (HEMAGGLUTININ "TOP" AND FAB FRAGMENT) ARE OBTAINED BY PROTEOLYSIS OF BIGGER ...THE TWO MOLECULES (HEMAGGLUTI | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.5 Å3/Da / Density % sol: 57 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() ![]() ![]() | |||||||||
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE | |||||||||
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 3.25→20 Å / Num. obs: 18144 / % possible obs: 96.4 % / Redundancy: 4.7 % / Rmerge(I) obs: 0.109 | |||||||||
Reflection | *PLUS Num. measured all: 85445 |
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Processing
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Refinement | Resolution: 3.25→7 Å / σ(F): 0 /
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Refinement step | Cycle: LAST / Resolution: 3.25→7 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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