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- PDB-4r4n: Crystal structure of the anti-hiv-1 antibody 2.2c in complex with... -

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Basic information

Entry
Database: PDB / ID: 4r4n
TitleCrystal structure of the anti-hiv-1 antibody 2.2c in complex with hiv-1 93ug037 gp120
Components
  • Antibody 2.2c LIGHT CHAIN
  • Antibody 2.2c heavy CHAIN
  • HIV-1 gp120
  • M48U1 peptide
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM/INHIBITOR / HIV-1 attachment glycoprotein / VIRAL PROTEIN-IMMUNE SYSTEM-INHIBITOR complex
Function / homology
Function and homology information


virus-mediated perturbation of host defense response => GO:0019049 / : / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / positive regulation of establishment of T cell polarity / host cell endosome membrane / clathrin-dependent endocytosis of virus by host cell / membrane => GO:0016020 / viral protein processing / fusion of virus membrane with host plasma membrane ...virus-mediated perturbation of host defense response => GO:0019049 / : / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / positive regulation of establishment of T cell polarity / host cell endosome membrane / clathrin-dependent endocytosis of virus by host cell / membrane => GO:0016020 / viral protein processing / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / structural molecule activity / virion attachment to host cell / host cell plasma membrane / virion membrane / plasma membrane
Similarity search - Function
HIV Envelope Protein Gp120; Chain G / Human immunodeficiency virus 1, Gp160, envelope glycoprotein / Envelope glycoprotein Gp160 / Retroviral envelope protein / Retroviral envelope protein GP41-like / Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120 / Beta Complex / Immunoglobulins ...HIV Envelope Protein Gp120; Chain G / Human immunodeficiency virus 1, Gp160, envelope glycoprotein / Envelope glycoprotein Gp160 / Retroviral envelope protein / Retroviral envelope protein GP41-like / Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120 / Beta Complex / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
CD4-MIMETIC MINIPROTEIN M48U1 / Envelope glycoprotein gp160
Similarity search - Component
Biological speciesHuman immunodeficiency virus 1
Homo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.56 Å
AuthorsAcharya, P. / Louder, R. / Kwong, P.D.
CitationJournal: J.Virol. / Year: 2014
Title: Structural Definition of an Antibody-Dependent Cellular Cytotoxicity Response Implicated in Reduced Risk for HIV-1 Infection.
Authors: Acharya, P. / Tolbert, W.D. / Gohain, N. / Wu, X. / Yu, L. / Liu, T. / Huang, W. / Huang, C.C. / Kwon, Y.D. / Louder, R.K. / Luongo, T.S. / McLellan, J.S. / Pancera, M. / Yang, Y. / Zhang, B. ...Authors: Acharya, P. / Tolbert, W.D. / Gohain, N. / Wu, X. / Yu, L. / Liu, T. / Huang, W. / Huang, C.C. / Kwon, Y.D. / Louder, R.K. / Luongo, T.S. / McLellan, J.S. / Pancera, M. / Yang, Y. / Zhang, B. / Flinko, R. / Foulke, J.S. / Sajadi, M.M. / Kamin-Lewis, R. / Robinson, J.E. / Martin, L. / Kwong, P.D. / Guan, Y. / DeVico, A.L. / Lewis, G.K. / Pazgier, M.
History
DepositionAug 19, 2014Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 5, 2014Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2018Group: Experimental preparation / Category: exptl_crystal_grow
Item: _exptl_crystal_grow.pdbx_details / _exptl_crystal_grow.temp
Revision 1.2Jul 29, 2020Group: Data collection / Derived calculations / Structure summary
Category: chem_comp / entity ...chem_comp / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / struct_conn / struct_site / struct_site_gen
Item: _chem_comp.name / _chem_comp.type ..._chem_comp.name / _chem_comp.type / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 1.3Jan 10, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / struct_conn
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: HIV-1 gp120
B: HIV-1 gp120
a: M48U1 peptide
L: Antibody 2.2c LIGHT CHAIN
I: HIV-1 gp120
H: Antibody 2.2c heavy CHAIN
b: M48U1 peptide
D: Antibody 2.2c LIGHT CHAIN
C: Antibody 2.2c heavy CHAIN
E: HIV-1 gp120
e: M48U1 peptide
G: Antibody 2.2c LIGHT CHAIN
F: Antibody 2.2c heavy CHAIN
i: M48U1 peptide
K: Antibody 2.2c LIGHT CHAIN
J: Antibody 2.2c heavy CHAIN
M: HIV-1 gp120
m: M48U1 peptide
O: Antibody 2.2c LIGHT CHAIN
N: Antibody 2.2c heavy CHAIN
P: HIV-1 gp120
p: M48U1 peptide
R: Antibody 2.2c LIGHT CHAIN
Q: Antibody 2.2c heavy CHAIN
S: HIV-1 gp120
s: M48U1 peptide
U: Antibody 2.2c LIGHT CHAIN
T: Antibody 2.2c heavy CHAIN
V: HIV-1 gp120
v: M48U1 peptide
X: Antibody 2.2c LIGHT CHAIN
W: Antibody 2.2c heavy CHAIN
hetero molecules


Theoretical massNumber of molelcules
Total (without water)713,45051
Polymers709,24732
Non-polymers4,20319
Water0
1
A: HIV-1 gp120
a: M48U1 peptide
L: Antibody 2.2c LIGHT CHAIN
H: Antibody 2.2c heavy CHAIN
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,5418
Polymers88,6564
Non-polymers8854
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: HIV-1 gp120
b: M48U1 peptide
D: Antibody 2.2c LIGHT CHAIN
C: Antibody 2.2c heavy CHAIN
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,0986
Polymers88,6564
Non-polymers4422
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
I: HIV-1 gp120
i: M48U1 peptide
K: Antibody 2.2c LIGHT CHAIN
J: Antibody 2.2c heavy CHAIN
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,5418
Polymers88,6564
Non-polymers8854
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
E: HIV-1 gp120
e: M48U1 peptide
G: Antibody 2.2c LIGHT CHAIN
F: Antibody 2.2c heavy CHAIN
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,0986
Polymers88,6564
Non-polymers4422
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
M: HIV-1 gp120
m: M48U1 peptide
O: Antibody 2.2c LIGHT CHAIN
N: Antibody 2.2c heavy CHAIN
hetero molecules


Theoretical massNumber of molelcules
Total (without water)88,8775
Polymers88,6564
Non-polymers2211
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
P: HIV-1 gp120
p: M48U1 peptide
R: Antibody 2.2c LIGHT CHAIN
Q: Antibody 2.2c heavy CHAIN
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,0986
Polymers88,6564
Non-polymers4422
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
S: HIV-1 gp120
s: M48U1 peptide
U: Antibody 2.2c LIGHT CHAIN
T: Antibody 2.2c heavy CHAIN
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,3207
Polymers88,6564
Non-polymers6643
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
8
V: HIV-1 gp120
v: M48U1 peptide
X: Antibody 2.2c LIGHT CHAIN
W: Antibody 2.2c heavy CHAIN
hetero molecules


Theoretical massNumber of molelcules
Total (without water)88,8775
Polymers88,6564
Non-polymers2211
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)112.967, 144.178, 158.351
Angle α, β, γ (deg.)110.58, 92.30, 99.19
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
22
33
44
/ NCS ensembles :
ID
1
2
3
4

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Components

#1: Protein
HIV-1 gp120


Mass: 38939.805 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus 1 / Cell line (production host): 293S / Production host: Homo sapiens (human) / References: UniProt: Q0ED04*PLUS
#2: Protein/peptide
M48U1 peptide


Type: Peptide-like / Class: Inhibitor / Mass: 3056.804 Da / Num. of mol.: 8 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) / References: CD4-MIMETIC MINIPROTEIN M48U1
#3: Antibody
Antibody 2.2c LIGHT CHAIN


Mass: 22912.578 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#4: Antibody
Antibody 2.2c heavy CHAIN


Mass: 23746.738 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#5: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-Acetylglucosamine


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 19
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
Compound detailsTHE CD4-MIMETIC MINIPROTEINS INHIBIT HIV-1 ENTRY AND ARE DERIVED FROM SCYLLATOXIN (A SCORPION TOXIN) ...THE CD4-MIMETIC MINIPROTEINS INHIBIT HIV-1 ENTRY AND ARE DERIVED FROM SCYLLATOXIN (A SCORPION TOXIN) BY TRANSPLANTING THE GP120-INTERACTIVE REGION OF CD4 ONTO THE SCYLLATOXIN SCAFFOLD, FOLLOWED BY MANY ROUNDS OF ITERATIVE OPTIMIZATION

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.34 Å3/Da / Density % sol: 63.22 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / pH: 4.5
Details: 14% PEG 3350, 1M sodium formate, 0.1M calcium chloride, and 100 mM sodium acetate pH 4.5 , VAPOR DIFFUSION, HANGING DROP, temperature 291 K

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Data collection

DiffractionMean temperature: 200 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Aug 10, 2009
RadiationMonochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.56→50 Å / Num. all: 102718 / Num. obs: 71903 / % possible obs: 70.2 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2
Reflection shellResolution: 3.78→4.07 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.276 / Mean I/σ(I) obs: 2.4 / % possible all: 52.3

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Processing

Software
NameVersionClassification
HKL-2000data collection
PHASERphasing
PHENIX(phenix.refine: 1.9_1692)refinement
HKL-2000data reduction
HKL-2000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.56→49.145 Å / SU ML: 0.59 / σ(F): 1.97 / Phase error: 44.11 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.3281 3541 5.14 %
Rwork0.2959 --
obs0.2975 68953 62.66 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.56→49.145 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms48886 0 266 0 49152
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00450365
X-RAY DIFFRACTIONf_angle_d0.98568337
X-RAY DIFFRACTIONf_dihedral_angle_d12.29818208
X-RAY DIFFRACTIONf_chiral_restr0.057756
X-RAY DIFFRACTIONf_plane_restr0.0058750
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.5601-3.60880.5125420.411590X-RAY DIFFRACTION14
3.6088-3.66040.3672330.3741644X-RAY DIFFRACTION15
3.6604-3.7150.3515400.3811785X-RAY DIFFRACTION19
3.715-3.7730.3875500.3592984X-RAY DIFFRACTION23
3.773-3.83480.4453550.36561136X-RAY DIFFRACTION27
3.8348-3.90090.3359700.37011446X-RAY DIFFRACTION34
3.9009-3.97180.3298800.36361620X-RAY DIFFRACTION39
3.9718-4.04820.3774990.37011913X-RAY DIFFRACTION46
4.0482-4.13080.3471320.3582173X-RAY DIFFRACTION52
4.1308-4.22060.40051370.35292377X-RAY DIFFRACTION57
4.2206-4.31870.3931310.33032504X-RAY DIFFRACTION60
4.3187-4.42660.35321660.3172611X-RAY DIFFRACTION63
4.4266-4.54620.32131510.31442817X-RAY DIFFRACTION67
4.5462-4.67990.3711560.31562961X-RAY DIFFRACTION70
4.6799-4.83080.35231660.30783042X-RAY DIFFRACTION74
4.8308-5.00330.33171870.29593201X-RAY DIFFRACTION77
5.0033-5.20350.38371740.30623397X-RAY DIFFRACTION81
5.2035-5.440.31351750.30113509X-RAY DIFFRACTION84
5.44-5.72640.3632070.29883599X-RAY DIFFRACTION87
5.7264-6.08450.34911990.31853799X-RAY DIFFRACTION91
6.0845-6.55330.36482160.32863991X-RAY DIFFRACTION95
6.5533-7.2110.32212210.32454125X-RAY DIFFRACTION99
7.211-8.25020.30012390.30924096X-RAY DIFFRACTION99
8.2502-10.37820.282270.24374113X-RAY DIFFRACTION98
10.3782-49.14930.29011880.24233979X-RAY DIFFRACTION95
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.62840.61090.06313.755-0.48393.7741-0.4576-1.31810.03060.33230.466-0.52070.10160.7828-0.01560.74040.2243-0.02061.7732-0.07411.166697.6715-14.5892-26.6504
22.9818-3.0888-1.15034.6058-2.68678.11950.14371.299-1.2071-0.09980.25161.65220.7052-1.5834-0.43051.5691-0.4518-0.05272.0887-0.19332.853463.4483-72.0916-60.6917
32.37220.25973.81698.0166-3.09016.0413-0.06290.6816-0.1711-0.5303-0.0142-0.70170.39960.68260.13761.1432-0.03850.28011.45750.00291.60384.9272-46.0398-50.3911
44.55450.5909-1.68995.92330.01264.9648-1.4515-1.4061-0.3332.08121.11650.09041.37381.39960.22691.64930.6671-0.25571.9072-0.03390.721963.7051-11.68698.2332
55.82930.69932.94022.0598-1.29035.72160.05871.3225-0.0593-5.4215-0.19071.0042-2.8391-1.529-0.3684.8430.56160.34421.9340.00682.150230.712748.3935-22.2516
66.0369-0.79441.3966.81553.43749.17270.10140.14340.28220.09240.3778-0.3044-0.46620.1298-0.30920.69430.03010.30290.95680.09631.385939.850419.9537-3.8558
76.54960.2651-2.13891.1659-0.65624.8273-0.57850.89950.4349-1.42270.33260.16020.8577-0.25570.25951.964-0.04430.0971.38670.30121.482812.7122-108.577417.013
83.7881-2.0402-0.75140.95630.46623.5312-1.2514-0.916-0.91913.26471.81742.0203-2.1080.4850.03234.22550.21991.25381.7950.52973.580732.3139-40.733940.3118
97.3164-2.09471.27344.5625-1.34834.86480.14870.34511.3332-1.2165-0.1264-0.568-0.11690.27020.0351.7429-0.01810.43911.09560.50083.297427.4577-70.861522.8337
102.86160.9986-0.29342.0831-0.56325.844-0.0867-0.89670.17570.63550.3694-0.2299-0.47661.2301-0.1391.1429-0.0132-0.03731.841-0.44211.19225.1534-40.6579-67.2027
110.04470.98460.58222.84731.4679-0.10880.0119-0.8627-0.6945-0.1743-0.43170.3019-1.5422-0.80390.48623.850.82340.90553.6481.12964.355-21.90593.8772-105.333
120.9331-1.73051.09328.7525-1.4532.01280.63571.08440.7382-0.05620.29430.5754-2.1114-3.2177-0.9972.59040.75440.9313.63690.15572.2055-5.3907-17.0328-82.2733
136.11560.072-0.54880.49631.20074.4958-0.03681.0165-0.0141-1.4057-0.28340.03760.6074-0.16130.34051.97520.15670.15761.64970.28751.250864.9773-5.1481-59.5907
147.72171.91552.05491.2389-1.69728.65130.5542-1.07261.88230.56310.02741.0474-0.68241.4572-0.48682.2941-0.6399-0.10482.2383-0.28652.1743103.224650.1874-27.0455
159.5768-3.94563.685.8947-0.40483.79040.6527-0.703-0.46320.5768-0.2108-0.4557-0.73430.9905-0.5121.6166-0.44520.21452.20750.59612.786388.886924.6945-45.1539
164.39980.7719-1.70173.3521-1.2975.39960.09940.50560.32450.19630.00461.74390.4589-1.1931-0.13190.8509-0.3969-0.20831.6748-0.31461.509533.3735-14.624-24.4696
172.69320.0581-1.02762.37215.02576.862-0.5833-0.1898-0.3657-2.81991.4785-4.9343-1.10880.209-0.12473.4893-0.10770.92881.5380.37353.567571.7115-73.66471.1742
183.92520.50771.01226.97232.73126.75940.1116-0.3188-0.5046-0.47090.12141.8843.0192-0.6635-0.1863.3305-0.3104-0.10351.13980.15141.819848.5844-47.8209-4.693
193.77240.8672-0.78515.036-1.30635.38850.1329-1.30170.8729-0.14330.2062-1.102-0.1391.4912-0.23310.7671-0.03320.2451.6457-0.35041.74825.571241.585941.4769
203.261.45521.12953.0856-0.9562.6214-1.60241.0121-5.96030.91260.04595.2017-0.284-1.6512-3.97762.7761-0.22282.49071.62390.18716.64762.7618-27.433922.9382
217.7536-0.70760.77058.6456-0.85496.1842-0.25630.0047-0.6601-0.13310.2739-0.25951.12390.178-0.15161.8340.20140.25561.1546-0.15820.819417.12824.519225.0999
222.8616-0.6041-1.12925.88410.23275.76860.29280.48480.51550.00630.24762.07960.2289-1.3897-0.33930.7767-0.3591-0.13931.58-0.02321.718-8.361-60.8976-91.5967
233.0479-1.87612.94365.6613-0.83728.06850.003-0.7703-0.6099-0.28580.0855-1.15551.1110.965-0.05971.7755-0.0263-0.36922.32530.38873.136138.7045-109.0449-57.3958
244.2014-0.89963.53879.31425.22246.3115-0.2129-0.5506-0.2757-0.55550.164-0.1686-0.24750.64140.0371.9194-0.46210.58831.78050.12031.816815.374-86.4902-68.8865
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND ( RESID 49:492 OR RESID 501:504 ) )A49 - 492
2X-RAY DIFFRACTION1( CHAIN A AND ( RESID 49:492 OR RESID 501:504 ) )A501 - 504
3X-RAY DIFFRACTION2( CHAIN H AND RESID 112:214 ) OR ( CHAIN L AND RESID 106:211 )H112 - 214
4X-RAY DIFFRACTION2( CHAIN H AND RESID 112:214 ) OR ( CHAIN L AND RESID 106:211 )L106 - 211
5X-RAY DIFFRACTION3( CHAIN H AND RESID 1:111 ) OR ( CHAIN L AND RESID 1:105 )H1 - 111
6X-RAY DIFFRACTION3( CHAIN H AND RESID 1:111 ) OR ( CHAIN L AND RESID 1:105 )L1 - 105
7X-RAY DIFFRACTION4( CHAIN B AND ( RESID 45:492 OR RESID 501:502 ) )B45 - 492
8X-RAY DIFFRACTION4( CHAIN B AND ( RESID 45:492 OR RESID 501:502 ) )B501 - 502
9X-RAY DIFFRACTION5( CHAIN C AND RESID 112:214 ) OR ( CHAIN D AND RESID 106:211 )C112 - 214
10X-RAY DIFFRACTION5( CHAIN C AND RESID 112:214 ) OR ( CHAIN D AND RESID 106:211 )D106 - 211
11X-RAY DIFFRACTION6( CHAIN C AND RESID 1:111 ) OR ( CHAIN D AND RESID 1:105 )C1 - 111
12X-RAY DIFFRACTION6( CHAIN C AND RESID 1:111 ) OR ( CHAIN D AND RESID 1:105 )D1 - 105
13X-RAY DIFFRACTION7( CHAIN E AND ( RESID 49:492 OR RESID 501:502 ) )E49 - 492
14X-RAY DIFFRACTION7( CHAIN E AND ( RESID 49:492 OR RESID 501:502 ) )E501 - 502
15X-RAY DIFFRACTION8( CHAIN G AND RESID 106:211 ) OR ( CHAIN F AND RESID 112:214 )G106 - 211
16X-RAY DIFFRACTION8( CHAIN G AND RESID 106:211 ) OR ( CHAIN F AND RESID 112:214 )F112 - 214
17X-RAY DIFFRACTION9( CHAIN G AND RESID 1:105 ) OR ( CHAIN F AND RESID 1:111 )G1 - 105
18X-RAY DIFFRACTION9( CHAIN G AND RESID 1:105 ) OR ( CHAIN F AND RESID 1:111 )F1 - 111
19X-RAY DIFFRACTION10( CHAIN I AND ( RESID 47:492 OR RESID 501:504 ) )I47 - 492
20X-RAY DIFFRACTION10( CHAIN I AND ( RESID 47:492 OR RESID 501:504 ) )I501 - 504
21X-RAY DIFFRACTION11( CHAIN K AND RESID 106:211 ) OR ( CHAIN J AND RESID 112:214 )K106 - 211
22X-RAY DIFFRACTION11( CHAIN K AND RESID 106:211 ) OR ( CHAIN J AND RESID 112:214 )J112 - 214
23X-RAY DIFFRACTION12( CHAIN K AND RESID 1:105 ) OR ( CHAIN J AND RESID 1:111 )K1 - 105
24X-RAY DIFFRACTION12( CHAIN K AND RESID 1:105 ) OR ( CHAIN J AND RESID 1:111 )J1 - 111
25X-RAY DIFFRACTION13( CHAIN M AND ( RESID 49:492 OR RESID 501:501 ) )M49 - 492
26X-RAY DIFFRACTION13( CHAIN M AND ( RESID 49:492 OR RESID 501:501 ) )M501
27X-RAY DIFFRACTION14( CHAIN O AND RESID 106:211 ) OR ( CHAIN N AND RESID 112:214 )O106 - 211
28X-RAY DIFFRACTION14( CHAIN O AND RESID 106:211 ) OR ( CHAIN N AND RESID 112:214 )N112 - 214
29X-RAY DIFFRACTION15( CHAIN O AND RESID 1:105 ) OR ( CHAIN N AND RESID 1:111 )O1 - 105
30X-RAY DIFFRACTION15( CHAIN O AND RESID 1:105 ) OR ( CHAIN N AND RESID 1:111 )N1 - 111
31X-RAY DIFFRACTION16( CHAIN P AND ( RESID 49:492 OR RESID 501:502 ) )P49 - 492
32X-RAY DIFFRACTION16( CHAIN P AND ( RESID 49:492 OR RESID 501:502 ) )P501 - 502
33X-RAY DIFFRACTION17( CHAIN Q AND RESID 112:214 ) OR ( CHAIN R AND RESID 106:211 )Q112 - 214
34X-RAY DIFFRACTION17( CHAIN Q AND RESID 112:214 ) OR ( CHAIN R AND RESID 106:211 )R106 - 211
35X-RAY DIFFRACTION18( CHAIN Q AND RESID 1:111 ) OR ( CHAIN R AND RESID 1:105 )Q1 - 111
36X-RAY DIFFRACTION18( CHAIN Q AND RESID 1:111 ) OR ( CHAIN R AND RESID 1:105 )R1 - 105
37X-RAY DIFFRACTION19( CHAIN S AND ( RESID 49:492 OR RESID 501:503 ) )S49 - 492
38X-RAY DIFFRACTION19( CHAIN S AND ( RESID 49:492 OR RESID 501:503 ) )S501 - 503
39X-RAY DIFFRACTION20( CHAIN U AND RESID 106:211 ) OR ( CHAIN T AND RESID 112:214 )U106 - 211
40X-RAY DIFFRACTION20( CHAIN U AND RESID 106:211 ) OR ( CHAIN T AND RESID 112:214 )T112 - 214
41X-RAY DIFFRACTION21( CHAIN U AND RESID 1:105 ) OR ( CHAIN T AND RESID 1:111 )U1 - 105
42X-RAY DIFFRACTION21( CHAIN U AND RESID 1:105 ) OR ( CHAIN T AND RESID 1:111 )T1 - 111
43X-RAY DIFFRACTION22( CHAIN V AND ( RESID 49:492 OR RESID 501:501 ) )V49 - 492
44X-RAY DIFFRACTION22( CHAIN V AND ( RESID 49:492 OR RESID 501:501 ) )V501
45X-RAY DIFFRACTION23( CHAIN X AND RESID 106:211 ) OR ( CHAIN W AND RESID 112:214 )X106 - 211
46X-RAY DIFFRACTION23( CHAIN X AND RESID 106:211 ) OR ( CHAIN W AND RESID 112:214 )W112 - 214
47X-RAY DIFFRACTION24( CHAIN X AND RESID 1:105 ) OR ( CHAIN W AND RESID 1:111 )X1 - 105
48X-RAY DIFFRACTION24( CHAIN X AND RESID 1:105 ) OR ( CHAIN W AND RESID 1:111 )W1 - 111

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