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- PDB-3gak: Structure of Giardia fructose-1,6-biphosphate aldolase -

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Basic information

Entry
Database: PDB / ID: 3gak
TitleStructure of Giardia fructose-1,6-biphosphate aldolase
ComponentsFructose-bisphosphate aldolase
KeywordsLYASE / Class II fructose-1 / 6-bisphosphate aldolase / glycolytic pathway / Giardia lamblia / drug target / Glycolysis
Function / homology
Function and homology information


fructose-bisphosphate aldolase / fructose-bisphosphate aldolase activity / glycolytic process / zinc ion binding
Similarity search - Function
Fructose-bisphosphate aldolase class-II signature 2. / Fructose-bisphosphate aldolase class-II / Fructose-bisphosphate aldolase, class-II / Aldolase class I / Aldolase-type TIM barrel / TIM Barrel / Alpha-Beta Barrel / Alpha Beta
Similarity search - Domain/homology
Putative fructose-1,6-bisphosphate aldolase
Similarity search - Component
Biological speciesGiardia intestinalis (unknown)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.9 Å
AuthorsGalkin, A. / Herzberg, O.
CitationJournal: Biochemistry / Year: 2009
Title: Structural insights into the substrate binding and stereoselectivity of giardia fructose-1,6-bisphosphate aldolase.
Authors: Galkin, A. / Li, Z. / Li, L. / Kulakova, L. / Pal, L.R. / Dunaway-Mariano, D. / Herzberg, O.
History
DepositionFeb 17, 2009Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 31, 2009Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Fructose-bisphosphate aldolase
B: Fructose-bisphosphate aldolase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)71,0438
Polymers70,5282
Non-polymers5156
Water0
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5370 Å2
ΔGint-168 kcal/mol
Surface area22010 Å2
MethodPISA
Unit cell
Length a, b, c (Å)62.920, 62.920, 318.590
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number169
Space group name H-MP61

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Components

#1: Protein Fructose-bisphosphate aldolase /


Mass: 35263.758 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Giardia intestinalis (unknown) / Strain: WB / Gene: ald, FBPA / Plasmid: pET100/D-TOPO / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)Star / References: UniProt: O97447, fructose-bisphosphate aldolase
#2: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#3: Chemical
ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: SO4
Sequence detailsAUTHORS STATE THAT ACCORDING TO THE SEQUENCE FROM GIARDIA GENOME (GB ENTRY EAA46366.1) THE CORRECT ...AUTHORS STATE THAT ACCORDING TO THE SEQUENCE FROM GIARDIA GENOME (GB ENTRY EAA46366.1) THE CORRECT RESIDUE AT POSITION 129 IS GLY.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.58 Å3/Da / Density % sol: 52.31 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: 18-25% PEG3350, 0.2M MgSO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.54178 Å
DetectorType: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Oct 22, 2007 / Details: mirrors
RadiationMonochromator: OSMIC MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.54178 Å / Relative weight: 1
ReflectionResolution: 2.9→10 Å / Num. all: 14110 / Num. obs: 12210 / % possible obs: 86.6 % / Rmerge(I) obs: 0.114
Reflection shellResolution: 2.9→3 Å / Rmerge(I) obs: 0.25 / % possible all: 98.7

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Processing

Software
NameVersionClassification
CrystalCleardata collection
PHASERphasing
REFMAC5refinement
CrystalCleardata reduction
CrystalCleardata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 2ISW
Resolution: 2.9→10 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
RfactorNum. reflection% reflectionSelection details
Rfree0.286 1072 -RANDOM
Rwork0.22 ---
all-14110 --
obs-12210 86.5 %-
Refinement stepCycle: LAST / Resolution: 2.9→10 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4514 0 22 0 4536
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_bond_d0.016
X-RAY DIFFRACTIONc_angle_deg1.7
LS refinement shellResolution: 2.9→2.97 Å /
RfactorNum. reflection
Rfree0.364 78
Rwork0.249 -
obs-980

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