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- PDB-6xc0: Crystal structure of bacteriophage T4 spackle and lysozyme in mon... -

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Basic information

Entry
Database: PDB / ID: 6xc0
TitleCrystal structure of bacteriophage T4 spackle and lysozyme in monoclinic form
Components
  • Lysozyme
  • Protein spackle
KeywordsHYDROLASE / lysozyme / spackle
Function / homology
Function and homology information


superinfection exclusion / host cell periplasmic space / symbiont entry into host cell via disruption of host cell wall peptidoglycan / virus tail, baseplate / symbiont entry into host cell via disruption of host cell envelope / viral tail assembly / virus tail / symbiont entry into host / peptidoglycan catabolic process / cell wall macromolecule catabolic process ...superinfection exclusion / host cell periplasmic space / symbiont entry into host cell via disruption of host cell wall peptidoglycan / virus tail, baseplate / symbiont entry into host cell via disruption of host cell envelope / viral tail assembly / virus tail / symbiont entry into host / peptidoglycan catabolic process / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to bacterium / symbiont entry into host cell / identical protein binding
Similarity search - Function
Protein spackle / Gp5, C-terminal / Gp5 C-terminal repeat (3 copies) / Protein Gp5, N-terminal OB-fold domain / Pre-baseplate central spike protein Gp5 / Gp5 N-terminal OB domain / Lysozyme - #40 / T4-type lysozyme / : / Glycoside hydrolase, family 24 ...Protein spackle / Gp5, C-terminal / Gp5 C-terminal repeat (3 copies) / Protein Gp5, N-terminal OB-fold domain / Pre-baseplate central spike protein Gp5 / Gp5 N-terminal OB domain / Lysozyme - #40 / T4-type lysozyme / : / Glycoside hydrolase, family 24 / Phage lysozyme / Lysozyme domain superfamily / Lysozyme / Lysozyme-like domain superfamily / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
FORMIC ACID / SUCCINIC ACID / Pre-baseplate central spike protein Gp5 / Protein spackle
Similarity search - Component
Biological speciesEscherichia virus T4
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.78 Å
AuthorsShi, K. / Oakland, J.T. / Kurniawan, F. / Moeller, N.H. / Aihara, H.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM118047 United States
CitationJournal: Commun Biol / Year: 2020
Title: Structural basis of superinfection exclusion by bacteriophage T4 Spackle.
Authors: Shi, K. / Oakland, J.T. / Kurniawan, F. / Moeller, N.H. / Banerjee, S. / Aihara, H.
History
DepositionJun 7, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 2, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Advisory / Data collection ...Advisory / Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_unobs_or_zero_occ_atoms / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_seq_id
Revision 1.2Oct 30, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Lysozyme
B: Lysozyme
C: Protein spackle
D: Protein spackle
hetero molecules


Theoretical massNumber of molelcules
Total (without water)65,64816
Polymers64,4144
Non-polymers1,23412
Water8,953497
1
A: Lysozyme
C: Protein spackle
hetero molecules


Theoretical massNumber of molelcules
Total (without water)32,7698
Polymers32,2072
Non-polymers5626
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Lysozyme
D: Protein spackle
hetero molecules


Theoretical massNumber of molelcules
Total (without water)32,8798
Polymers32,2072
Non-polymers6726
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)41.310, 127.597, 46.088
Angle α, β, γ (deg.)90.000, 92.245, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 276 through 292 or resid 294 through 324 or resid 326 through 341))
d_2ens_1(chain "B" and (resid 276 through 292 or resid 294 through 324 or resid 326 through 341))
d_1ens_2(chain "C" and (resid 23 through 28 or resid 30...
d_2ens_2(chain "D" and (resid 23 through 28 or resid 30...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11ens_1ALAALAPHEPHEAA276 - 292103 - 119
d_12ens_1THRTHRALAALAAA294 - 324121 - 151
d_13ens_1ARGARGVALVALAA326 - 341153 - 168
d_21ens_1ALAALAPHEPHEBB276 - 292103 - 119
d_22ens_1THRTHRALAALABB294 - 324121 - 151
d_23ens_1ARGARGVALVALBB326 - 341153 - 168
d_11ens_2GLYGLYLEULEUCC23 - 2823 - 28
d_12ens_2GLUGLUVALVALCC30 - 6330 - 63
d_13ens_2LYSLYSTYRTYRCC65 - 8065 - 80
d_14ens_2ASPASPGLUGLUCC82 - 9782 - 97
d_21ens_2GLYGLYLEULEUDD23 - 2823 - 28
d_22ens_2GLUGLUVALVALDD30 - 6330 - 63
d_23ens_2LYSLYSTYRTYRDD65 - 8065 - 80
d_24ens_2ASPASPGLUGLUDD82 - 9782 - 97

NCS ensembles :
ID
ens_1
ens_2

NCS oper:
IDCodeMatrixVector
1given(0.99997316721, -0.00588985569741, 0.00435596833749), (-0.0059107119852, -0.999971055891, 0.0047907059717), (0.00432762569101, -0.00481632429795, -0.999979037118)18.951937014, 32.7935977049, -23.5611777041
2given(0.997083079121, -0.0178245429379, 0.0742133343763), (-0.01858287462, -0.999781806094, 0.0095402816724), (0.0740270903187, -0.0108915505134, -0.997196752916)20.7229941364, 32.6875926631, -22.1967726456

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Components

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Protein , 2 types, 4 molecules ABCD

#1: Protein Lysozyme / Baseplate central spike complex protein gp5 / Peptidoglycan hydrolase gp5 / Protein Gp5


Mass: 20131.152 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia virus T4 / Production host: Escherichia coli (E. coli) / References: UniProt: P16009, lysozyme
#2: Protein Protein spackle


Mass: 12075.799 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia virus T4 / Gene: sp, 61.3 / Production host: Escherichia coli (E. coli) / References: UniProt: P39230

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Non-polymers , 6 types, 509 molecules

#3: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C2H6O2
#4: Chemical ChemComp-EPE / 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / HEPES


Mass: 238.305 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C8H18N2O4S / Comment: pH buffer*YM
#5: Chemical ChemComp-SIN / SUCCINIC ACID


Mass: 118.088 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H6O4
#6: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Cl
#7: Chemical ChemComp-FMT / FORMIC ACID


Mass: 46.025 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: CH2O2
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 497 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 1.88 Å3/Da / Density % sol: 34.72 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: 35% (w/v) polyethylene glycol 3350, 20% 2-propanol, 0.1 M HEPES-NaOH pH 7.5

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Mar 1, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 1.78→127.43 Å / Num. obs: 44738 / % possible obs: 98.6 % / Redundancy: 4.9 % / Biso Wilson estimate: 25.15 Å2 / CC1/2: 0.995 / Rmerge(I) obs: 0.113 / Net I/σ(I): 13.1
Reflection shellResolution: 1.78→1.88 Å / Rmerge(I) obs: 0.924 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 6478 / CC1/2: 0.708

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Processing

Software
NameVersionClassification
PHENIX1.18_3861refinement
XDSdata reduction
XDSdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6x6o, 1wth

6x6o

1wth


Resolution: 1.78→63.8 Å / SU ML: 0.2522 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 20.476
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.1983 2286 5.11 %
Rwork0.156 42407 -
obs0.1581 44693 98.35 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 29.12 Å2
Refinement stepCycle: LAST / Resolution: 1.78→63.8 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3838 0 75 497 4410
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01274047
X-RAY DIFFRACTIONf_angle_d1.17335444
X-RAY DIFFRACTIONf_chiral_restr0.0625585
X-RAY DIFFRACTIONf_plane_restr0.0073701
X-RAY DIFFRACTIONf_dihedral_angle_d21.04021569
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.289632917377
ens_2d_2CX-RAY DIFFRACTIONTorsion NCS0.571348749527
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.78-1.820.37671430.3042542X-RAY DIFFRACTION96.27
1.82-1.860.27981630.26882639X-RAY DIFFRACTION97.9
1.86-1.910.31781500.24712633X-RAY DIFFRACTION98.27
1.91-1.960.28131450.22842657X-RAY DIFFRACTION98.66
1.96-2.020.29541190.22012638X-RAY DIFFRACTION97.21
2.02-2.080.23041220.18132681X-RAY DIFFRACTION99.15
2.08-2.160.21111550.17382646X-RAY DIFFRACTION98.73
2.16-2.240.20891450.16672644X-RAY DIFFRACTION98.9
2.24-2.350.22291460.14972663X-RAY DIFFRACTION99.08
2.35-2.470.22341340.14642629X-RAY DIFFRACTION97.39
2.47-2.620.18121500.14752688X-RAY DIFFRACTION99.2
2.62-2.830.20381500.14932646X-RAY DIFFRACTION99.33
2.83-3.110.18421340.15482684X-RAY DIFFRACTION98.88
3.11-3.560.16431470.13642664X-RAY DIFFRACTION98.22
3.56-4.490.17181210.12212700X-RAY DIFFRACTION99.02
4.49-63.80.15791620.14192653X-RAY DIFFRACTION97.51
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
19.15394925147-4.91153532066-1.929194704395.25732018865.902846872379.52003773836-0.0167060826687-0.384033195798-0.3070552314560.103534656820.04026956182770.4830129437530.221524512843-0.4411013915220.04397808841110.2329684597030.0119109478360.005102586340040.2146808862210.05726750432690.197000766108-25.90145958022.94676309893-18.2536925659
27.45998062742-0.381192060045-1.903597835745.365124192914.645435962236.836157292710.0127690632245-0.001443769640050.243638688429-0.2411336779780.141581492955-0.167664154931-0.2441964547410.324951071252-0.1167376194740.18113380019-0.0122054529218-0.02686099083750.1527809418840.02226269280190.134648610194-12.5446856909-2.47358266934-12.704005961
38.64895896775-4.71320343908-2.411257921453.079352777150.4184964256472.407141381860.0436010463558-0.1297969068520.1081093012190.06982761660490.0738584746758-0.428356654079-0.1060442094311.14236235209-0.07072744487330.255467895504-0.0120864072479-0.01128181029880.389412257064-0.02866387103810.251582903637-4.444592591430.890853822424-9.58974471448
47.31757503563-0.6009767329681.032775025488.74685104766-2.761405675866.078942178650.0881697236303-0.0288661734749-0.104357199840.151067157999-0.0613283375441-0.2507143784560.1272036646170.382338182422-0.02742421777940.2078998236280.00569825854227-0.02491215756490.187442452546-0.002731445495780.0991285258547-11.1867410405-3.01406362015-0.735027847263
51.74204990991-0.22603947546-0.2769678901349.4543900637-7.676455069628.30152602734-0.237791149981-0.1675624460350.2316293678060.7423999549970.299141631595-0.299879509603-0.777611975003-0.362955667115-0.06581332253240.2741052372820.0763205293844-0.02827770593590.259265737111-0.04943851887510.183646946254-21.59025702069.38927537174-8.49842013393
61.65323924348-0.703256296378-0.3224174752251.98465055438-0.4987002786372.90860003435-0.0112576985560.03612607229270.2065871680720.1130811519830.0158509269171-0.10280943423-0.1847373544860.0691122257059-0.008248173029660.170959883403-0.01672269138240.004392871659520.161729155950.01000400428060.197590144837-20.831854814914.721929116-27.3622702088
78.97634475871-5.762385562025.517542107593.70890780973-3.529400854713.40940364770.240249624613-0.148792014476-0.4661034143780.348465986798-0.001653249120590.8410551757440.232990390848-0.561563819573-0.2750668509060.201684383531-0.02542770867270.01650024270140.2774211151910.03109052928220.295291746182-32.33206243066.5838947239-27.2535438758
87.564757244615.50570868586-3.070681978378.638653820440.3555569055262.69218584108-0.04787611442220.5155071773390.2563916580490.0232444880310.2370424257710.3201194738710.303701584998-0.378614273852-0.1511591159970.225799696586-0.0295728890713-0.01116374662970.2777505269520.03739369505010.191792155384-6.7967756900529.9314177747-5.69219161386
97.94169793552.28268342614-1.616088142364.272802454842.143297923866.76174628781-0.0126166711295-0.090165739266-0.3164219386570.08597767316910.0745082162368-0.121316101970.439367761440.0863543128618-0.08262854837640.224293091142-0.007260129381560.008039219013340.1492132260370.003923256732960.1419051067337.145279837734.8589703821-10.1145651322
101.993304594440.72628771860.8189112872077.985889300974.990675514388.96340082999-0.0308245540216-0.716642013561-0.2596983438110.2695642358380.307684941654-0.3697985443270.4408456020371.65247653202-0.1999550200970.2114870721650.03754930298760.001535074342890.481795501633-0.001107589487570.28888450483515.292414891430.9821640926-12.5980082715
117.90574711224-2.029421381551.30399323886.60653185077-0.892962336879.26009033514-0.1212211025430.2779189110410.0039884046097-0.38419844852-0.0110120104392-0.3257757357830.1680820785790.4255020922120.1598845721140.238922569713-0.0334317296610.04130669277380.188837608170.01937384219510.1481549330669.5912068645935.8259835862-22.1064639338
120.9011289737710.4385463998-0.3993084543356.06241602406-5.036688748444.0618541859-0.2120140850490.137335916679-0.0775960133467-0.7514527210490.220465375909-0.2109544884350.672106717487-0.344685387454-0.02822182204440.319286812495-0.08979064714320.01949322335870.308716917979-0.01100990964460.217091713745-2.2748710650623.6597968267-15.3876654306
133.59429047005-0.435974752739-0.6090048393133.167055424160.1260918331723.1069247162-0.08815444134040.0637126545095-0.0979177834524-0.04020654012430.0205761032538-0.08091163191140.07549060523540.05538583636540.0557242335340.203312197385-0.02365891732760.005191782560060.1667908613640.001406934159950.153084057838-2.8846953030315.9556385243-2.60846426785
143.786955424023.4651661705-0.3551232444026.89894515319-0.6971754945242.564668782330.13579127996-0.183247855241-0.2710952501230.166507319061-0.168345083389-0.249864138283-0.01137463609350.0940690381430.03595548528490.1433112935280.0165871413538-0.0290483887070.1883374334750.0413191517760.1936915759580.15209822412717.10471036938.99303761293
156.3607698234.95313179988-1.009919713456.65544829141-1.681498813680.4838309608650.0130805414539-0.3541808552320.1393041413530.0767649110541-0.06863456905210.0485992526144-0.201221906294-0.04507883347170.04051032180380.2286410786370.0202568698001-0.005643084684450.2153893807040.01022626834840.163608097273-5.1422333787324.7533839966.50024236441
169.126959009876.88626568221-4.34033945688.02804287789-3.268741985742.258212081610.1231027479070.3643918842350.5495185611310.1744186235550.1636541953960.672820183553-0.458180565264-0.838085724846-0.2518562342480.2456704797150.04811235482930.02022109771220.2716143053450.01236202483810.267087176671-13.466928052126.26256964413.48766213174
173.86016637445-2.161513455235.058233738794.46937701178-1.574114028287.1666773960.0642819986399-0.661756758551-0.6513732259650.4068559106550.2345303823650.4990552486630.367849501298-0.52394071769-0.2886273759930.280877006344-0.0135274773540.01675408202710.236483331220.05207307074480.262377726392-23.0956334149-14.4732751497-14.0307807336
182.3979861912.944245223212.007251618724.023130564152.812750524851.998460915170.1009499518970.249552965723-0.0808665593733-0.0901079748658-0.0103573889326-0.1330667436970.3913327387010.196255640725-0.1459073097930.2267926847470.02240862312960.02065093183620.196265569573-0.004017185450730.217235324323-16.9557869999-14.2022901032-26.8728267365
199.273696858237.78174512994.363481413387.702005247455.772931027535.94352235043-0.1185365898410.231910956266-0.092362807972-0.1643491258150.06156888563440.01747866293360.134959305461-0.008295600794070.03517850867750.2756542809840.04470398121260.01139151887510.2200594116380.03206149216720.224092899597-13.8331547867-6.4054507048-34.6529237298
205.034958783162.83524971048-1.734925006585.78149625842-0.04457025882887.120041834980.05072170472310.2062054117320.0613588558849-0.1250993936530.0564766272730.250467536753-0.198138271528-0.344977744492-0.1495139454450.1972982514490.04381691834890.004606657200660.1474955985660.0009626824993610.173432628661-24.5542596098-6.44501512489-25.3515001925
214.159468629662.30915238492-5.765872487087.75477380952-0.3520263520099.277573761030.1304263860130.4714926354120.391372448624-0.6002951113910.2029072636020.588915654274-0.294352131864-0.914824540838-0.3048893341430.30574993353-0.00311167661489-0.07295284778350.2964427239680.07778140609440.246685394375-2.9618212414247.4830889505-9.49872721415
225.20231806659-2.40613238813-4.281715761483.168066642123.003715124418.99599117750.049748453103-0.1847618812250.292291702862-0.02976511319160.148902851942-0.0268336079882-0.2691854652060.277395831568-0.218818960350.19879996438-0.0247830356769-0.04676775826050.14822946523-0.002549896582560.2038562020622.0007938360246.90483479763.35753863056
236.52327053782-5.96981030851-6.80105530629.278934431397.57309115498.52716316763-0.149107989733-0.1371605411470.008486507465510.2179949252890.1127230425490.04187076694120.1043491479230.179745435153-0.0007712605272220.229431740951-0.0253909487599-0.03609734460470.209338280560.03145637602660.1857251536314.6638367783739.304776735911.2125133772
249.44252768197-4.05901094756-0.2980776302488.08937596262-0.08473155929895.25262352721-0.1450656092250.377416981782-0.0877182592923-0.2960837454420.09575572773450.5354291157440.14351861843-0.5480198655030.04975740031920.190106531057-0.0362185329512-0.03257517851690.206219863220.01644100547140.155442772636-5.4315280330540.5486343037-2.90961622649
255.51036259887-4.912982303773.41866313918.94899268390.09861719252964.29921207209-0.220357955326-0.198156497191-0.4052267058580.6036851450520.235006621490.547350248640.204428965987-0.455719612564-0.0568382601320.260359123326-0.01549076665770.05354358705520.251079178330.02307209001210.225570683089-5.4474487111537.72173465417.68792716453
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 175 through 184 )AA175 - 1841 - 10
22chain 'A' and (resid 185 through 204 )AA185 - 20411 - 31
33chain 'A' and (resid 205 through 213 )AA205 - 21332 - 40
44chain 'A' and (resid 214 through 237 )AA214 - 23741 - 64
55chain 'A' and (resid 238 through 258 )AA238 - 25865 - 85
66chain 'A' and (resid 259 through 333 )AA259 - 33386 - 162
77chain 'A' and (resid 334 through 341 )AA334 - 341163 - 170
88chain 'B' and (resid 175 through 184 )BE175 - 1841 - 10
99chain 'B' and (resid 185 through 204 )BE185 - 20411 - 30
1010chain 'B' and (resid 205 through 213 )BE205 - 21331 - 39
1111chain 'B' and (resid 214 through 237 )BE214 - 23740 - 64
1212chain 'B' and (resid 238 through 258 )BE238 - 25865 - 85
1313chain 'B' and (resid 259 through 291 )BE259 - 29186 - 119
1414chain 'B' and (resid 292 through 320 )BE292 - 320120 - 149
1515chain 'B' and (resid 321 through 333 )BE321 - 333150 - 162
1616chain 'B' and (resid 334 through 341 )BE334 - 341163 - 170
1717chain 'C' and (resid 23 through 36 )CH23 - 361 - 15
1818chain 'C' and (resid 37 through 55 )CH37 - 5516 - 34
1919chain 'C' and (resid 56 through 70 )CH56 - 7035 - 49
2020chain 'C' and (resid 71 through 98 )CH71 - 9850 - 78
2121chain 'D' and (resid 23 through 36 )DJ23 - 361 - 15
2222chain 'D' and (resid 37 through 55 )DJ37 - 5516 - 34
2323chain 'D' and (resid 56 through 70 )DJ56 - 7035 - 50
2424chain 'D' and (resid 71 through 86 )DJ71 - 8651 - 67
2525chain 'D' and (resid 87 through 97 )DJ87 - 9768 - 78

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