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- PDB-3bwz: Crystal structure of the type II cohesin module from the cellulos... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3bwz | ||||||
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Title | Crystal structure of the type II cohesin module from the cellulosome of Acetivibrio cellulolyticus with an extended linker conformation | ||||||
![]() | Cellulosomal scaffoldin adaptor protein B | ||||||
![]() | STRUCTURAL PROTEIN / COHESINS II / CELLULOSOME | ||||||
Function / homology | ![]() polysaccharide catabolic process / hydrolase activity, hydrolyzing O-glycosyl compounds / carbohydrate binding / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Noach, I. / Lamed, R. / Shimon, L.J.W. / Bayer, E. / Frolow, F. | ||||||
![]() | ![]() Title: Intermodular linker flexibility revealed from crystal structures of adjacent cellulosomal cohesins of Acetivibrio cellulolyticus. Authors: Noach, I. / Frolow, F. / Alber, O. / Lamed, R. / Shimon, L.J. / Bayer, E.A. #1: ![]() Title: Preliminary x-ray characterisation and phasing of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus Authors: Noach, I. / Lamed, R. / Xu, Q. / Rosenheck, S. / Shimon, L.J.W. / Bayer, E. / Frolow, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 97.8 KB | Display | ![]() |
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PDB format | ![]() | 74.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1zv9SC ![]() 3fnkC ![]() 3ghpC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 19590.951 Da / Num. of mol.: 1 / Fragment: COHESIN II DOMAIN FROM CELLULOSOME ASSEMBLY Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
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#2: Chemical | ChemComp-NO3 / | ||||
#3: Chemical | #4: Chemical | ChemComp-EDO / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.52 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 2.0 M ammonium sulfate and 0.1 M sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Dec 14, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.2→47.09 Å / Num. all: 53407 / Num. obs: 53407 / % possible obs: 95.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4 % / Biso Wilson estimate: 10.1 Å2 / Rmerge(I) obs: 0.046 / Rsym value: 0.046 / Net I/σ(I): 13 |
Reflection shell | Resolution: 1.2→1.22 Å / Redundancy: 4 % / Rmerge(I) obs: 0.17 / Mean I/σ(I) obs: 4 / Num. unique all: 2848 / Rsym value: 0.17 / % possible all: 99.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1ZV9 Resolution: 1.2→47.09 Å / Cor.coef. Fo:Fc: 0.979 / Cor.coef. Fo:Fc free: 0.968 / SU B: 0.859 / SU ML: 0.018 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.033 / ESU R Free: 0.034 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 9.558 Å2
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Refinement step | Cycle: LAST / Resolution: 1.2→47.09 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.2→1.231 Å / Total num. of bins used: 20
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