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- PDB-1g1k: COHESIN MODULE FROM THE CELLULOSOME OF CLOSTRIDIUM CELLULOLYTICUM -
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Open data
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Basic information
Entry | Database: PDB / ID: 1g1k | ||||||
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Title | COHESIN MODULE FROM THE CELLULOSOME OF CLOSTRIDIUM CELLULOLYTICUM | ||||||
![]() | SCAFFOLDING PROTEIN | ||||||
![]() | STRUCTURAL PROTEIN / Beta -Barrel | ||||||
Function / homology | ![]() hydrolase activity, acting on glycosyl bonds / cellulose binding / cellulose catabolic process / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Spinelli, S. / Fierobe, H.-P. / Belaich, A. / Belaich, J.-P. / Henrissat, B. / Cambillau, C. | ||||||
![]() | ![]() Title: Crystal structure of a cohesin module from Clostridium cellulolyticum: implications for dockerin recognition. Authors: Spinelli, S. / Fierobe, H.P. / Belaich, A. / Belaich, J.P. / Henrissat, B. / Cambillau, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 66.4 KB | Display | ![]() |
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PDB format | ![]() | 50.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 378.1 KB | Display | ![]() |
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Full document | ![]() | 388.2 KB | Display | |
Data in XML | ![]() | 8.4 KB | Display | |
Data in CIF | ![]() | 13.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | MONOMER, POSSIBLY A DIMER |
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Components
#1: Protein | Mass: 14648.539 Da / Num. of mol.: 2 / Fragment: RESIDUES 287 - 428 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.83 % | ||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 8000 (30%), 150 mM (NH4)2SO4, 100 mM guanidine hydrochloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||
Crystal | *PLUS Density % sol: 50 % | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Details: used macroseeding | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 22, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.988 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. all: 24484 / Num. obs: 22829 / % possible obs: 98 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 3.5 % / Biso Wilson estimate: 27 Å2 / Rmerge(I) obs: 0.082 / Net I/σ(I): 6.4 |
Reflection shell | Resolution: 2→2.06 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.275 / % possible all: 97.8 |
Reflection | *PLUS % possible obs: 98 % |
Reflection shell | *PLUS % possible obs: 98.8 % / Mean I/σ(I) obs: 2 |
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Processing
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Refinement | Resolution: 2→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / σ(F): 0 / % reflection Rfree: 4.2 % / Rfactor obs: 0.23 / Rfactor Rfree: 0.27 / Rfactor Rwork: 0.23 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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