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- PDB-2wwm: Crystal structure of the Titin M10-Obscurin like 1 Ig complex in ... -

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Basic information

Entry
Database: PDB / ID: 2wwm
TitleCrystal structure of the Titin M10-Obscurin like 1 Ig complex in space group P1
Components
  • OBSCURIN-LIKE PROTEIN 1
  • TITIN
KeywordsTRANSFERASE/STRUCTURAL PROTEIN / TRANSFERASE-STRUCTURAL PROTEIN COMPLEX / SARCOMERE / TPR REPEAT / IMMUNOGLOBULIN DOMAIN / LIMB-GIRDLE MUSCULAR DYSTROPHY / SERINE/THREONINE-PROTEIN KINASE / TRANSFERASE / CARDIOMYOPATHY / CALMODULIN-BINDING / NUCLEOTIDE-BINDING
Function / homology
Function and homology information


3M complex / sarcomerogenesis / structural molecule activity conferring elasticity / telethonin binding / skeletal muscle myosin thick filament assembly / protein localization to Golgi apparatus / cardiac myofibril assembly / detection of muscle stretch / muscle alpha-actinin binding / cytoskeletal anchor activity ...3M complex / sarcomerogenesis / structural molecule activity conferring elasticity / telethonin binding / skeletal muscle myosin thick filament assembly / protein localization to Golgi apparatus / cardiac myofibril assembly / detection of muscle stretch / muscle alpha-actinin binding / cytoskeletal anchor activity / cardiac muscle tissue morphogenesis / regulation of catalytic activity / Striated Muscle Contraction / mitotic chromosome condensation / cardiac muscle hypertrophy / M band / actinin binding / I band / cardiac muscle cell development / regulation of protein kinase activity / positive regulation of dendrite morphogenesis / structural constituent of muscle / sarcomere organization / regulation of mitotic nuclear division / Golgi organization / skeletal muscle thin filament assembly / striated muscle thin filament / intercalated disc / striated muscle contraction / protein kinase A signaling / cytoskeleton organization / cardiac muscle contraction / muscle contraction / condensed nuclear chromosome / positive regulation of protein secretion / microtubule cytoskeleton organization / Z disc / response to calcium ion / : / actin filament binding / Platelet degranulation / Neddylation / protein tyrosine kinase activity / protease binding / calmodulin binding / non-specific serine/threonine protein kinase / phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / centrosome / calcium ion binding / positive regulation of gene expression / protein kinase binding / perinuclear region of cytoplasm / Golgi apparatus / enzyme binding / extracellular exosome / extracellular region / ATP binding / identical protein binding / cytosol / cytoplasm
Similarity search - Function
PPAK motif / PPAK motif / Titin, Z repeat / Titin Z / MyBP-C, tri-helix bundle domain / Tri-helix bundle domain / Immunoglobulin domain / Immunoglobulin I-set / Immunoglobulin I-set domain / Fibronectin type III domain ...PPAK motif / PPAK motif / Titin, Z repeat / Titin Z / MyBP-C, tri-helix bundle domain / Tri-helix bundle domain / Immunoglobulin domain / Immunoglobulin I-set / Immunoglobulin I-set domain / Fibronectin type III domain / Fibronectin type 3 domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Immunoglobulin V-Type / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Tyrosine-protein kinase, active site / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Protein kinase domain / Immunoglobulins / Immunoglobulin-like fold / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Obscurin-like protein 1 / Titin
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å
AuthorsPernigo, S. / Fukuzawa, A. / Gautel, M. / Steiner, R.A.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2010
Title: Structural Insight Into M-Band Assembly and Mechanics from the Titin-Obscurin-Like-1 Complex.
Authors: Pernigo, S. / Fukuzawa, A. / Bertz, M. / Holt, M. / Rief, M. / Steiner, R.A. / Gautel, M.
History
DepositionOct 26, 2009Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 16, 2010Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Advisory / Version format compliance
Revision 1.2Aug 24, 2011Group: Derived calculations
Revision 1.3Dec 20, 2023Group: Data collection / Database references ...Data collection / Database references / Other / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf
Remark 700 SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
C: OBSCURIN-LIKE PROTEIN 1
D: TITIN
O: OBSCURIN-LIKE PROTEIN 1
T: TITIN


Theoretical massNumber of molelcules
Total (without water)44,2144
Polymers44,2144
Non-polymers00
Water2,036113
1
O: OBSCURIN-LIKE PROTEIN 1
T: TITIN


Theoretical massNumber of molelcules
Total (without water)22,1072
Polymers22,1072
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1300 Å2
ΔGint-7.1 kcal/mol
Surface area10360 Å2
MethodPISA
2
C: OBSCURIN-LIKE PROTEIN 1
D: TITIN


Theoretical massNumber of molelcules
Total (without water)22,1072
Polymers22,1072
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1230 Å2
ΔGint-7.53 kcal/mol
Surface area10810 Å2
MethodPISA
Unit cell
Length a, b, c (Å)37.650, 44.940, 68.010
Angle α, β, γ (deg.)87.29, 86.91, 68.79
Int Tables number1
Space group name H-MP1

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Components

#1: Protein OBSCURIN-LIKE PROTEIN 1


Mass: 11125.619 Da / Num. of mol.: 2 / Fragment: RESIDUES 1-106
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): ROSETTA 2 / References: UniProt: O75147
#2: Protein TITIN / / CONNECTIN / RHABDOMYOSARCOMA ANTIGEN MU-RMS-40.14


Mass: 10981.231 Da / Num. of mol.: 2 / Fragment: M10, RESIDUES 34252-34350
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): ROSETTA 2
References: UniProt: Q8WZ42, non-specific serine/threonine protein kinase
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 113 / Source method: isolated from a natural source / Formula: H2O
Sequence detailsINITIAL GSS ARE FROM THE EXPRESSION VECTOR

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.49 Å3/Da / Density % sol: 50.27 % / Description: NONE
Crystal growpH: 5 / Details: 0.1 M MIB BUFFER PH 5.0, 25% PEG 1500

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9763
DetectorType: ADSC CCD / Detector: CCD / Date: Aug 12, 2009 / Details: MIRRORS
RadiationMonochromator: SI (111) DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9763 Å / Relative weight: 1
ReflectionResolution: 2.3→36.13 Å / Num. obs: 17094 / % possible obs: 92.6 % / Observed criterion σ(I): 0 / Redundancy: 3.8 % / Biso Wilson estimate: 47.3 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 13.2
Reflection shellResolution: 2.3→2.42 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.25 / Mean I/σ(I) obs: 4.3 / % possible all: 93.1

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Processing

Software
NameVersionClassification
REFMAC5.5.0109refinement
MOSFLMdata reduction
SCALAdata scaling
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 2WP3

2wp3


Resolution: 2.3→35.88 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.92 / SU B: 17.898 / SU ML: 0.192 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 0.418 / ESU R Free: 0.263 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES RESIDUAL ONLY. ATOM RECORD CONTAINS RESIDUAL B FACTORS ONLY
RfactorNum. reflection% reflectionSelection details
Rfree0.25219 879 5.1 %RANDOM
Rwork0.19718 ---
obs0.19997 16215 92.66 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK
Displacement parametersBiso mean: 17.149 Å2
Baniso -1Baniso -2Baniso -3
1--1.16 Å2-1.11 Å20.03 Å2
2--3.93 Å2-1.46 Å2
3----1.83 Å2
Refinement stepCycle: LAST / Resolution: 2.3→35.88 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2917 0 0 113 3030
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.010.0223025
X-RAY DIFFRACTIONr_bond_other_d0.0010.022039
X-RAY DIFFRACTIONr_angle_refined_deg1.2351.9774133
X-RAY DIFFRACTIONr_angle_other_deg0.78735005
X-RAY DIFFRACTIONr_dihedral_angle_1_deg5.8375402
X-RAY DIFFRACTIONr_dihedral_angle_2_deg29.29423.917120
X-RAY DIFFRACTIONr_dihedral_angle_3_deg14.09615469
X-RAY DIFFRACTIONr_dihedral_angle_4_deg14.6451521
X-RAY DIFFRACTIONr_chiral_restr0.070.2475
X-RAY DIFFRACTIONr_gen_planes_refined0.0060.0213415
X-RAY DIFFRACTIONr_gen_planes_other0.0010.02574
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it0.3051.51981
X-RAY DIFFRACTIONr_mcbond_other0.0781.5799
X-RAY DIFFRACTIONr_mcangle_it0.56323198
X-RAY DIFFRACTIONr_mcangle_other
X-RAY DIFFRACTIONr_scbond_it0.9631044
X-RAY DIFFRACTIONr_scbond_other
X-RAY DIFFRACTIONr_scangle_it1.4944.5930
X-RAY DIFFRACTIONr_scangle_other
X-RAY DIFFRACTIONr_long_range_B_refined
X-RAY DIFFRACTIONr_long_range_B_other
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 2.3→2.36 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.36 67 -
Rwork0.262 1254 -
obs--92.77 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.6115-3.31310.690310.0151-7.94854.17660.06710.0371-0.4136-0.07310.32680.78690.1817-0.5054-0.39380.2612-0.0253-0.0020.436-0.0060.25057.650414.882-39.7016
28.2331-7.3232-1.3155-9.69612.33914.5697-0.3887-1.1573-1.92060.16280.25580.09081.0495-1.35340.13290.2884-0.08920.05610.33530.13170.460316.66028.8281-31.7085
370.1092-16.9708-8.367310.12828.40969.615-0.7736-1.78560.60021.23320.6837-0.33560.84760.4830.08990.4079-0.0395-0.04880.17080.0520.277931.94297.31-29.7992
428.0682-12.32448.60954.9689-1.21835.85240.36620.3031-2.52050.2669-0.01050.76850.7812-0.097-0.35570.3159-0.0123-0.05510.3971-0.07190.614827.54084.126-37.3196
57.0454-7.66028.84044.6362-7.630827.4471-0.48850.1114-0.0080.14570.17730.03430.45660.64970.31120.2571-0.09480.02140.4103-0.05240.29829.267410.3984-41.4142
618.1972-2.1824-0.03840.4421-2.37957.5276-1.07720.8149-0.4783-0.06530.3818-0.0271-0.5655-0.32890.69540.3121-0.2094-0.10670.33620.02470.17715.773314.6113-45.4018
7-0.4179-1.4564.73613.68624.259933.1555-0.19070.24430.11540.15910.6409-0.5438-1.33731.7956-0.45020.314-0.1152-0.03840.3251-0.00320.3228.206320.049-43.4127
844.85-30.97172.475131.7189-6.7047-1.39820.74690.0759-0.3415-0.9949-0.7133-0.85270.2630.3763-0.03360.2789-0.0174-0.09930.5152-0.3570.580432.55849.357-47.9823
927.5764-1.76433.08725.75321.44861.57710.36230.2682-1.09290.6965-0.12870.02460.2334-0.0321-0.23360.2429-0.01670.02920.2382-0.03980.208220.95755.9064-46.1685
1039.9936-3.35643.1356-0.2303-0.28460.36260.1388-0.0355-1.49060.1169-0.11380.14310.12580.1012-0.02490.2451-0.05970.07420.3056-0.05470.28319.67056.4672-42.1201
1117.013413.64558.293729.07322.611614.0552-0.37860.2331-1.3582-0.49671.0123-1.0321-0.14390.9122-0.63370.30830.00540.00580.44380.04130.23836.56338.8353-39.2714
126.1241-3.62163.7941.757-2.62010.53790.04650.05060.0980.0136-0.1688-0.1726-0.10840.0710.12230.1978-0.0642-0.05930.25360.05010.218729.307815.4653-36.5203
1370.47076.490227.03620.3002-4.883836.3428-0.89742.35031.6819-0.28750.3539-0.1308-0.88641.0150.54360.5301-0.1320.23840.1932-0.14390.246114.456219.4634-44.6076
1428.4876-4.122922.72871.0731-5.596825.3181-0.1962-0.18140.38350.0452-0.10030.0735-0.02450.11660.29650.24530.04510.01660.1878-0.1210.107413.013320.524-40.7531
1545.668-14.447511.61334.4256-2.29966.83210.16640.50330.377-0.1014-0.0751-0.2881-0.27180.3939-0.09130.2259-0.07330.01250.18460.05270.292838.48779.0768-29.8127
1611.04184.9458-0.30361.6046-8.681626.3771-0.5941-0.7118-0.6733-0.9926-0.9187-1.04732.30731.8961.51270.3020.1070.12110.34710.13910.328723.913514.6372-12.4955
175.93151.3925-0.22990.7501-2.28987.4102-0.0580.1999-0.1453-0.3309-0.2218-0.20980.62640.65940.27980.29670.0449-0.00480.2032-0.03980.24720.322823.2285-33.3193
1812.4355-5.31287.49358.0231-7.726210.3012-0.21580.48890.455-0.21560.04540.0821-0.4394-0.26850.17040.27740.0305-0.05180.1838-0.04560.09069.763425.3296-39.6952
19-1.99323.25362.28469.131111.00821.47830.21950.1091-0.32940.9754-0.38830.08850.9606-0.25810.16880.3149-0.01440.05660.17050.07780.360518.247614.899-17.0904
203.39080.50230.910213.0688-7.93212.05950.0666-0.3107-0.13040.3357-0.0766-0.1463-0.537-0.31480.010.1228-0.022-0.01830.0738-0.00590.06413.502623.656-20.2564
219.23985.54154.344433.607214.04946.83920.20590.4490.5060.244-0.33760.77530.172-0.21790.13160.25540.0529-0.05980.12960.04590.18211.561834.9468-24.0203
2217.02354.7705-16.319311.1164-1.079522.7987-0.31090.75150.82910.12140.25021.5330.0863-0.9580.06070.2791-0.0104-0.06660.4869-0.01960.7826-0.25728.2436-26.0232
239.4353-1.7961-3.3591-3.29945.4873-0.72110.0961-0.67590.4421-0.19450.17280.1912-0.7129-0.3143-0.2690.42740.070.00490.3935-0.04460.18543.862128.0742-31.4084
2417.67571.2734-18.31910.9473-3.721621.5879-0.4354-0.1048-1.2558-0.33190.34050.831.2249-0.78790.09490.2563-0.2971-0.15380.27310.17160.689211.054415.8507-23.8602
2512.4023-12.32389.026421.6821-7.28923.7615-0.21420.4602-0.33510.05440.34460.7401-0.20070.1278-0.13040.24140.0623-0.00060.2528-0.0280.10246.53425.0937-34.183
2612.39786.3929-3.013125.5101-2.3748-0.8725-0.44710.74940.908-0.32590.52440.9364-0.0438-0.385-0.07730.4960.1258-0.09310.28610.07470.32648.526735.6569-38.7489
2710.2562-2.91195.07290.77281.75381.65790.28220.59880.7672-0.3898-0.6467-0.469-0.19690.08680.36460.26340.02570.06250.16270.00420.170817.282128.9646-26.6405
281.0903-1.40136.818611.1674-0.028214.8722-0.2326-0.12320.21280.9633-0.2453-0.3697-0.332-0.01140.47780.1222-0.0638-0.01990.27340.05160.115220.004620.9668-10.6594
294.88620.17025.94956.6423-2.4736.4578-0.29280.23250.16060.26630.0174-0.2236-0.56140.33910.27530.227-0.03470.0330.18510.0090.065720.639528-28.5947
3012.19531.5186-0.4626-4.0634-1.658525.2444-0.0260.69140.5916-0.3857-0.01880.1415-1.0602-0.82860.04480.93740.0419-0.18060.4526-0.06120.321114.917332.2779-45.2655
311.61482.26476.374613.6814-4.47664.56230.03370.21480.2257-0.02680.48921.34210.0518-0.173-0.52290.14090.01920.03030.22320.01090.2621-10.24183.01785.4069
3214.93384.2844-14.77986.27212.94496.22480.63922.63870.9129-1.1402-0.94881.7223-1.0422-2.09570.30960.32620.2409-0.18110.6653-0.05910.5144-1.27239.8092-1.4168
3317.34353.7044-5.73484.2226-0.56120.34760.12020.3510.1921-0.3947-0.01190.0537-0.0494-0.0854-0.10830.1998-0.0332-0.01410.23240.08850.181513.61413.5988-3.4177
343.86025.2881-4.91849.02780.39497.43770.3554-0.07491.29270.31620.0931.5717-0.46890.0553-0.44850.13690.01040.01450.14370.06240.37268.826415.1774.1993
3530.5818.3028-21.782911.0655-11.038929.29160.034-0.01750.30430.13060.08970.1346-0.82080.6755-0.12370.22310.0273-0.05840.07850.01360.1891-9.08447.47677.577
3620.771-2.7247-6.1566-0.66191.20521.2392-0.2458-1.1455-0.41070.05980.07230.01770.02870.26630.17350.1004-0.02620.0510.2203-0.0310.2054-6.49033.94713.4998
3733.0286-6.51273.64298.016.59448.51660.13090.56020.10110.6869-0.4307-0.27011.9740.34330.29980.64070.22520.28380.15920.18040.18897.86321.67087.3249
383.485210.709-1.447928.01533.562517.22540.12150.2-0.62181.010.1374-1.43971.0814-0.656-0.25890.1918-0.0091-0.04360.15540.02050.268310.51351.568313.0164
397.6730.0148-3.82396.50435.73676.48110.6872-0.61950.31280.4567-0.1833-0.39880.11330.1834-0.50390.12690.0079-0.00630.16820.04380.135810.793611.466213.2154
4037.17573.1164-4.40462.51630.01020.458-0.11660.42921.65260.18680.22730.4155-0.0242-0.027-0.11070.17960.02380.00860.10770.01960.12911.656712.60449.5648
4110.7677-2.44795.29878.6157-0.24540.76150.13290.5071-0.0646-0.1442-0.0988-0.5834-0.06770.1728-0.0340.1275-0.022-0.01470.14470.09410.160918.802210.4645.3891
4226.73758.8958-3.24973.2369-0.20894.43230.21860.0996-0.1286-0.1362-0.1150.0613-0.0822-0.0763-0.10360.18690.0872-0.06040.1355-0.01740.1588.97343.35112.8776
4360.173218.5105-18.9516.722-2.800310.14320.2061-2.6344-2.48810.2502-0.7576-0.904-0.0020.97180.55150.151-0.0480.02370.1780.03160.1928-4.0482-1.536611.7629
4413.211-3.64413.99371.573.48958.5294-1.0387-0.1911-0.34421.4350.95430.10661.69611.08270.08440.6180.27810.10170.2758-0.02180.0232-4.636-2.58136.5163
4542.85599.924-9.76412.9251-1.02128.7741-0.4231.7027-1.1353-0.1810.3131-0.5530.3760.52570.10990.11850.03650.04920.27910.05790.220118.043110.7936-3.0862
466.55442.7645-3.78159.5889-4.2487.7002-0.3349-0.00540.22430.1134-0.0219-0.8054-0.66590.47290.35680.2763-0.0769-0.0570.21960.01440.13265.59326.051-21.5324
475.67995.4456-5.545.906-10.888424.29290.5252-0.0825-0.03081.1392-0.5424-0.1271-1.49291.05740.01720.3888-0.0877-0.0330.20310.01610.19434.4162-2.9435-2.3927
483.10311.75024.66236.4028-2.20619.590.5267-0.3457-0.28630.7454-0.15180.46050.45570.0346-0.3750.31660.01960.0920.14730.10160.2554-7.9798-10.2789.0956
493.03233.5928-6.42710.37-13.221617.4168-0.18130.20920.028-0.25230.0464-0.0940.3882-0.26420.13480.0851-0.0129-0.02560.1299-0.08630.1221-3.063-0.6025-4.1092
50-0.98363.0694-2.5908-2.18061.7997-5.1283-0.3601-1.241.4371-0.92-0.37820.9868-0.9281-0.67570.73830.63930.1732-0.24550.3291-0.2110.5425-0.66464.7739-19.0837
516.70615.2054-5.85768.1623-0.30775.9854-0.29160.1885-0.1093-0.05750.07820.22020.4337-0.38250.21340.18050.0016-0.03580.1014-0.03610.0952-4.0654-7.7469-11.7128
527.3232-0.3195-3.458510.76687.5152.8543-0.0007-0.1049-0.2182-0.5037-0.21910.7637-0.202-0.16340.21980.3571-0.010.02710.21340.00670.2475-6.7635-14.639-10.7731
5314.769412.133527.992732.754628.31918.35693.27570.3645-0.3762-0.8908-2.90222.30022.5102-1.8336-0.37350.59420.117-0.09510.81750.1890.9864-17.8857-10.7985-4.9516
5414.123513.814-12.115231.8187-24.467118.3819-0.40310.60180.64690.26171.30771.2540.0473-1.2-0.90460.2239-0.0561-0.04020.2867-0.09240.1559-11.388-5.006-6.4748
5515.9923.3115-4.063333.1787-43.333148.38140.28230.90073.3857-1.41021.01441.3971.3996-0.8162-1.29670.42340.1553-0.16010.14380.06020.7297-5.52824.0281-9.7032
5614.448712.3499-8.710214.4456-14.780124.21960.06530.44830.77060.10460.71771.3430.5123-1.2889-0.7830.1899-0.0590.08750.1227-0.03370.4015-11.161-7.75910.4136
5733.12713.2544-8.162431.40326.9805-0.2505-0.32940.8647-1.64590.6559-0.23220.78210.2451-0.29260.56160.34140.0310.04140.098-0.01660.2734-7.6814-17.35323.8582
585.34411.7747-4.71331.17060.92281.8309-0.1791-0.1946-0.31320.1334-0.1136-0.12650.34430.1580.29270.17480.029-0.00040.0605-0.01440.11740.4031-8.0598-9.9935
590.77211.3781-1.78642.8185-8.124729.4458-0.23430.1256-0.1289-0.1153-0.0666-0.2057-0.18310.57110.3010.16170.03450.04540.1350.0250.16184.0257-3.4636-16.5243
6013.972212.5409-3.503611.9673-2.4715-7.44250.5479-1.2643-0.49621.2342-0.7408-0.0747-0.12270.6090.19290.5120.20920.15040.33990.12820.2964-0.4494-12.41746.2179
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1C9 - 15
2X-RAY DIFFRACTION2C16 - 19
3X-RAY DIFFRACTION3C20 - 25
4X-RAY DIFFRACTION4C26 - 32
5X-RAY DIFFRACTION5C33 - 38
6X-RAY DIFFRACTION6C39 - 46
7X-RAY DIFFRACTION7C47 - 52
8X-RAY DIFFRACTION8C53 - 58
9X-RAY DIFFRACTION9C59 - 64
10X-RAY DIFFRACTION10C65 - 70
11X-RAY DIFFRACTION11C71 - 77
12X-RAY DIFFRACTION12C78 - 83
13X-RAY DIFFRACTION13C84 - 88
14X-RAY DIFFRACTION14C89 - 96
15X-RAY DIFFRACTION15C97 - 106
16X-RAY DIFFRACTION16D0 - 6
17X-RAY DIFFRACTION17D7 - 16
18X-RAY DIFFRACTION18D17 - 26
19X-RAY DIFFRACTION19D27 - 32
20X-RAY DIFFRACTION20D33 - 39
21X-RAY DIFFRACTION21D40 - 45
22X-RAY DIFFRACTION22D46 - 50
23X-RAY DIFFRACTION23D51 - 55
24X-RAY DIFFRACTION24D56 - 62
25X-RAY DIFFRACTION25D63 - 68
26X-RAY DIFFRACTION26D69 - 74
27X-RAY DIFFRACTION27D75 - 81
28X-RAY DIFFRACTION28D82 - 87
29X-RAY DIFFRACTION29D88 - 94
30X-RAY DIFFRACTION30D95 - 99
31X-RAY DIFFRACTION31O9 - 15
32X-RAY DIFFRACTION32O16 - 19
33X-RAY DIFFRACTION33O20 - 25
34X-RAY DIFFRACTION34O26 - 32
35X-RAY DIFFRACTION35O33 - 38
36X-RAY DIFFRACTION36O39 - 44
37X-RAY DIFFRACTION37O45 - 48
38X-RAY DIFFRACTION38O49 - 55
39X-RAY DIFFRACTION39O56 - 62
40X-RAY DIFFRACTION40O63 - 71
41X-RAY DIFFRACTION41O72 - 78
42X-RAY DIFFRACTION42O79 - 84
43X-RAY DIFFRACTION43O85 - 89
44X-RAY DIFFRACTION44O90 - 95
45X-RAY DIFFRACTION45O96 - 105
46X-RAY DIFFRACTION46T0 - 6
47X-RAY DIFFRACTION47T7 - 14
48X-RAY DIFFRACTION48T15 - 21
49X-RAY DIFFRACTION49T22 - 28
50X-RAY DIFFRACTION50T29 - 34
51X-RAY DIFFRACTION51T35 - 40
52X-RAY DIFFRACTION52T41 - 47
53X-RAY DIFFRACTION53T48 - 52
54X-RAY DIFFRACTION54T53 - 57
55X-RAY DIFFRACTION55T58 - 63
56X-RAY DIFFRACTION56T64 - 68
57X-RAY DIFFRACTION57T69 - 74
58X-RAY DIFFRACTION58T75 - 83
59X-RAY DIFFRACTION59T84 - 92
60X-RAY DIFFRACTION60T93 - 97

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