+Open data
-Basic information
Entry | Database: PDB / ID: 2a38 | ||||||
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Title | Crystal structure of the N-Terminus of titin | ||||||
Components | Titin | ||||||
Keywords | STRUCTURAL PROTEIN / Titin / Z1Z2 | ||||||
Function / homology | Function and homology information sarcomerogenesis / structural molecule activity conferring elasticity / telethonin binding / skeletal muscle myosin thick filament assembly / cardiac myofibril assembly / muscle alpha-actinin binding / detection of muscle stretch / cardiac muscle tissue morphogenesis / cardiac muscle hypertrophy / mitotic chromosome condensation ...sarcomerogenesis / structural molecule activity conferring elasticity / telethonin binding / skeletal muscle myosin thick filament assembly / cardiac myofibril assembly / muscle alpha-actinin binding / detection of muscle stretch / cardiac muscle tissue morphogenesis / cardiac muscle hypertrophy / mitotic chromosome condensation / Striated Muscle Contraction / actinin binding / M band / I band / protein kinase regulator activity / cardiac muscle cell development / structural constituent of muscle / sarcomere organization / skeletal muscle thin filament assembly / striated muscle thin filament / condensed nuclear chromosome / striated muscle contraction / muscle contraction / cardiac muscle contraction / protein kinase A signaling / positive regulation of protein secretion / response to calcium ion / Z disc / actin filament binding / Platelet degranulation / protein tyrosine kinase activity / protease binding / calmodulin binding / non-specific serine/threonine protein kinase / protein serine kinase activity / protein serine/threonine kinase activity / calcium ion binding / positive regulation of gene expression / protein kinase binding / enzyme binding / protein homodimerization activity / extracellular exosome / extracellular region / ATP binding / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Marino, M. / Muhle-Goll, C. / Svergun, D. / Demirel, M. / Mayans, O. | ||||||
Citation | Journal: Structure / Year: 2006 Title: The Ig doublet Z1Z2: a model system for the hybrid analysis of conformational dynamics in Ig tandems from titin Authors: Marino, M. / Zou, P. / Svergun, D. / Garcia, P. / Edlich, C. / Simon, B. / Wilmanns, M. / Muhle-Goll, C. / Mayans, O. #1: Journal: Biophys.J. / Year: 2007 Title: Secondary and tertiary structure elasticity of titin Z1Z2 and a titin chain model Authors: Lee, E.H. / Hsin, J. / Mayans, O. / Schulten, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2a38.cif.gz | 130.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2a38.ent.gz | 101.3 KB | Display | PDB format |
PDBx/mmJSON format | 2a38.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2a38_validation.pdf.gz | 442 KB | Display | wwPDB validaton report |
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Full document | 2a38_full_validation.pdf.gz | 451.8 KB | Display | |
Data in XML | 2a38_validation.xml.gz | 28.2 KB | Display | |
Data in CIF | 2a38_validation.cif.gz | 41 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a3/2a38 ftp://data.pdbj.org/pub/pdb/validation_reports/a3/2a38 | HTTPS FTP |
-Related structure data
Related structure data | 1fhgS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 20733.117 Da / Num. of mol.: 3 / Fragment: Ig-like 1/2, residues 1-194 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: pET-M11 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) References: UniProt: Q8WZ42, non-specific serine/threonine protein kinase #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.6 Å3/Da / Density % sol: 65.4 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: MES, Cadmium sulphate, Sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 0.92 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Details: Dynamically bendable mirror |
Radiation | Monochromator: LN2 cooled fixed-exit Si(111) monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 51011 / % possible obs: 91.4 % / Observed criterion σ(I): 1.5 / Redundancy: 1.8 % / Biso Wilson estimate: 20.35 Å2 / Rsym value: 0.092 / Net I/σ(I): 8.9 |
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 1.4 % / Mean I/σ(I) obs: 2.8 / Rsym value: 0.349 / % possible all: 69.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1FHG Resolution: 2→20 Å / Stereochemistry target values: Engh & Huber
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refine LS restraints |
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