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- PDB-2wk4: Dimeric structure of D347G D348G mutant of the sapporovirus RNA d... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2wk4 | ||||||
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Title | Dimeric structure of D347G D348G mutant of the sapporovirus RNA dependent RNA polymerase | ||||||
![]() | PROTEASE-POLYMERASE P70 | ||||||
![]() | HYDROLASE / COVALENT PROTEIN-RNA LINKAGE / NUCLEOTIDYLTRANSFERASE / RNA REPLICATION / PHOSPHORYLATION / RNA ELONGATION / THIOL PROTEASE / CAPSID PROTEIN / ATP-BINDING / HELICASE / PROTEASE | ||||||
Function / homology | ![]() calicivirin / ribonucleoside triphosphate phosphatase activity / viral capsid / nucleoside-triphosphate phosphatase / DNA replication / host cell cytoplasm / RNA helicase activity / RNA-directed RNA polymerase / viral RNA genome replication / cysteine-type endopeptidase activity ...calicivirin / ribonucleoside triphosphate phosphatase activity / viral capsid / nucleoside-triphosphate phosphatase / DNA replication / host cell cytoplasm / RNA helicase activity / RNA-directed RNA polymerase / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / DNA-templated transcription / proteolysis / RNA binding / ATP binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fullerton, S.W.B. / Robel, I. / Schuldt, L. / Gebhardt, J. / Tucker, P.A. / Rohayem, J. | ||||||
![]() | ![]() Title: Dimeric Structure of D347G D348G Mutant of the Sapporovirus Sapporovirus RNA Dependent RNA Polymerase Authors: Fullerton, S.W.B. / Robel, I. / Schuldt, L. / Gebhardt, J. / Tucker, P.A. / Rohayem, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 201.9 KB | Display | ![]() |
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PDB format | ![]() | 162.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 458.5 KB | Display | ![]() |
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Full document | ![]() | 491.1 KB | Display | |
Data in XML | ![]() | 41.4 KB | Display | |
Data in CIF | ![]() | 57.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ckwS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Ens-ID: 1 / Beg auth comp-ID: LYS / Beg label comp-ID: LYS / End auth comp-ID: GLY / End label comp-ID: GLY / Refine code: 6 / Auth seq-ID: 12 - 502 / Label seq-ID: 12 - 502
NCS oper: (Code: given Matrix: (-0.9097, -0.3897, 0.1432), Vector: |
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Components
#1: Protein | Mass: 56369.633 Da / Num. of mol.: 2 / Fragment: RESIDUES 1135-1649 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Compound details | ENGINEERED RESIDUE IN CHAIN A, ASP 1481 TO GLY ENGINEERED RESIDUE IN CHAIN A, ASP 1482 TO GLY ...ENGINEERED | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.49 % / Description: NONE |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR555 FLAT PANEL / Detector: IMAGE PLATE / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.81 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→50 Å / Num. obs: 29506 / % possible obs: 96 % / Observed criterion σ(I): -3 / Redundancy: 7.4 % / Biso Wilson estimate: 61.1 Å2 / Rmerge(I) obs: 0.21 / Net I/σ(I): 10.8 |
Reflection shell | Resolution: 2.9→3.06 Å / Redundancy: 6.3 % / Rmerge(I) obs: 0.99 / Mean I/σ(I) obs: 2 / % possible all: 72.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2CKW Resolution: 2.98→26.01 Å / Cor.coef. Fo:Fc: 0.927 / Cor.coef. Fo:Fc free: 0.837 / SU B: 18.818 / SU ML: 0.353 / Cross valid method: THROUGHOUT / ESU R Free: 0.484 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.478 Å2
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Refinement step | Cycle: LAST / Resolution: 2.98→26.01 Å
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Refine LS restraints |
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