+Open data
-Basic information
Entry | Database: PDB / ID: 4nz0 | ||||||
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Title | The EMCV 3Dpol structure at 2.8A resolution | ||||||
Components | Genome polyprotein | ||||||
Keywords | TRANSFERASE / Encephalomyocarditis virus / Close right hand / RNa dependent RNA polymerase | ||||||
Function / homology | Function and homology information positive stranded viral RNA replication / host cell nucleolus / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity / picornain 3C / T=pseudo3 icosahedral viral capsid / host cell cytoplasmic vesicle membrane / : / protein complex oligomerization / monoatomic ion channel activity / RNA helicase activity ...positive stranded viral RNA replication / host cell nucleolus / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity / picornain 3C / T=pseudo3 icosahedral viral capsid / host cell cytoplasmic vesicle membrane / : / protein complex oligomerization / monoatomic ion channel activity / RNA helicase activity / RNA helicase / induction by virus of host autophagy / symbiont entry into host cell / RNA-directed RNA polymerase / symbiont-mediated suppression of host gene expression / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / DNA-templated transcription / virion attachment to host cell / structural molecule activity / ATP hydrolysis activity / proteolysis / RNA binding / ATP binding / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | Mengo virus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Vives-adrian, L. / Ferrer-orta, C. / Verdaguer, N. | ||||||
Citation | Journal: J.Virol. / Year: 2014 Title: The crystal structure of a cardiovirus RNA-dependent RNA polymerase reveals an unusual conformation of the polymerase active site Authors: Vives-Adrian, L. / Lujan, C. / Oliva, B. / van der Linden, L. / Selisko, B. / Coutard, B. / Canard, B. / van Kuppeveld, F.J. / Ferrer-Orta, C. / Verdaguer, N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4nz0.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb4nz0.ent.gz | 928.8 KB | Display | PDB format |
PDBx/mmJSON format | 4nz0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nz/4nz0 ftp://data.pdbj.org/pub/pdb/validation_reports/nz/4nz0 | HTTPS FTP |
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-Related structure data
Related structure data | 4nyzC 1u09S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 52244.504 Da / Num. of mol.: 6 / Fragment: UNP residues 1834-2293 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mengo virus / Plasmid: pETG20A / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta (DE3) pLyS / References: UniProt: P12296, RNA-directed RNA polymerase #2: Chemical | ChemComp-GOL / #3: Chemical | ChemComp-CL / | #4: Water | ChemComp-HOH / | Sequence details | AUTHOR STATES THE CONFLICT IS DUE TO THE USED CONSTRUCT. | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.58 Å3/Da / Density % sol: 65.61 % |
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Crystal grow | Temperature: 293 K / pH: 4.6 Details: 4.0M Ammonium acetate, 0.1M Sodium acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALBA / Beamline: XALOC / Wavelength: 0.979 |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Feb 2, 2013 |
Radiation | Monochromator: CHANNEL-CUT SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.69→138.2 Å / Num. obs: 119540 / % possible obs: 98 % / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 2.69→2.84 Å / Redundancy: 2.4 % / Rmerge(I) obs: 0.62 / Mean I/σ(I) obs: 16.3 / Rsym value: 0.49 / % possible all: 92.5 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1U09 Resolution: 2.8→138.2 Å / Cor.coef. Fo:Fc: 0.931 / Cor.coef. Fo:Fc free: 0.917 / SU B: 49.959 / SU ML: 0.409 / Cross valid method: THROUGHOUT / ESU R: 0.92 / ESU R Free: 0.358 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 92.72 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→138.2 Å
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Refine LS restraints |
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