Mass: 19589.703 Da / Num. of mol.: 2 / Fragment: RESIDUES 37-190 Source method: isolated from a genetically manipulated source Source: (gene. exp.) VACCINIA VIRUS / Strain: WESTERN RESERVE / Plasmid: POPINF / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): ROSETTA(DE3) PLYSS / References: UniProt: Q01220
Sequence details
RESIDUES 1-36 ARE REMOVED, AN N-TERMINAL START CODON IS ADDED AND A C-TERMINAL LYS-HIS6 ...RESIDUES 1-36 ARE REMOVED, AN N-TERMINAL START CODON IS ADDED AND A C-TERMINAL LYS-HIS6 PURIFICATION TAG IS ADDED.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 4.88 Å3/Da / Density % sol: 74.83 % Description: MODEL WAS STRIPPED OF SOLVENT AND ALTERNATE CONFORMATIONS PRIOR TO MOLECULAR REPLACEMENT.
Crystal grow
Temperature: 294 K / Method: vapor diffusion, hanging drop Details: HANGING DROPS CONTAINING 1 UL PROTEIN (5.4 MG/ML IN 20 MM TRIS, 200 MM NACL, PH 7.5) AND 1 UL RESERVOIR SOLUTION (0.2 M SODIUM PHOSPHATE, 22% (W/V) PEG 3350) WERE EQUILIBRATED AGAINST 500 UL ...Details: HANGING DROPS CONTAINING 1 UL PROTEIN (5.4 MG/ML IN 20 MM TRIS, 200 MM NACL, PH 7.5) AND 1 UL RESERVOIR SOLUTION (0.2 M SODIUM PHOSPHATE, 22% (W/V) PEG 3350) WERE EQUILIBRATED AGAINST 500 UL RESERVOIRS AT 21 C. CRYSTALS WERE CRYOPROTECTED IN RESERVOIR SOLUTION SUPPLEMENTED WITH 20% (V/V) GLYCEROL.
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