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Open data
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Basic information
Entry | Database: PDB / ID: 2uxe | ||||||
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Title | The structure of Vaccinia virus N1 | ||||||
![]() | HYPOTHETICAL PROTEIN![]() | ||||||
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Function / homology | ![]() | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cooray, S. / Bahar, M.W. / Abrescia, N.G.A. / McVey, C.E. / Bartlett, N.W. / Chen, R.A.-J. / Stuart, D.I. / Grimes, J.M. / Smith, G.L. | ||||||
![]() | ![]() Title: Functional and Structural Studies of the Vaccinia Virus Virulence Factor N1 Reveal a Bcl-2-Like Anti- Apoptotic Protein Authors: Cooray, S. / Bahar, M.W. / Abrescia, N.G.A. / Mcvey, C.E. / Bartlett, N.W. / Chen, R.A.-J. / Stuart, D.I. / Grimes, J.M. / Smith, G.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 143.4 KB | Display | ![]() |
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PDB format | ![]() | 121.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 14290.240 Da / Num. of mol.: 6 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() Compound details | ENGINEERED RESIDUE IN CHAIN A, CYS 40 TO SER ENGINEERED RESIDUE IN CHAIN B, CYS 40 TO SER ...ENGINEERED | Sequence details | CYS 40 WAS MUTATED TO SER SEMET WAS INCORPORAT | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 61.21 % |
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Crystal grow![]() | pH: 6.2 Details: 0.2 M NA/K PHOSPHATE, 0.2 M NACL AND 10% PEG 8000, PH 6.2 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.9→30 Å / Num. obs: 22319 / % possible obs: 100 % / Observed criterion σ(I): -5 / Redundancy: 11.9 % / Rmerge(I) obs: 0.14 / Net I/σ(I): 17.9 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 11.4 % / Mean I/σ(I) obs: 1.8 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 70 Å2 / ksol: 0.31 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 75 Å2
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Refinement step | Cycle: LAST / Resolution: 2.9→30 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: CONSTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.9→2.95 Å / Total num. of bins used: 21
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Xplor file | Serial no: 1 / Param file: PROTEIN_REP.PARAM |