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- PDB-3owe: Crystal Structure of Staphylococcal Enterotoxin G (SEG) in Comple... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3owe | ||||||
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Title | Crystal Structure of Staphylococcal Enterotoxin G (SEG) in Complex with a High Affinity Mutant Mouse T-cell Receptor Chain | ||||||
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![]() | IMMUNE SYSTEM / T-CELL RECEPTOR V BETA DOMAIN / COMPLEX STRUCTURE / IgG domains / B grasp / virulence factors | ||||||
Function / homology | ![]() T cell receptor complex / : / toxin activity / adaptive immune response / cell surface receptor signaling pathway / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fernandez, M.M. / Cho, S. / Robinson, H. / Mariuzza, R.A. / Malchiodi, M.L. | ||||||
![]() | ![]() Title: Crystal structure of staphylococcal enterotoxin G (SEG) in complex with a mouse T-cell receptor {beta} chain. Authors: Fernandez, M.M. / Cho, S. / De Marzi, M.C. / Kerzic, M.C. / Robinson, H. / Mariuzza, R.A. / Malchiodi, E.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 528.7 KB | Display | ![]() |
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PDB format | ![]() | 438.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3mc0C ![]() 2aq3S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
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Components
#1: Protein | Mass: 12100.324 Da / Num. of mol.: 8 / Fragment: VARIABLE DOMAIN Mutation: G17E, A52V, S54N, K66E, Q72H, E80V, L81S, T87S, G96V Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 27088.725 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.06 Å3/Da / Density % sol: 69.69 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.2 M sodium citrate, 30% iso-propanol and 0.1 M sodium cacodylate, Protein concentration: 3.25 mg/ml in the drop, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.5 |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Mar 1, 2007 Details: Cryogenically cooled double crystal monochrometer with horizontal focusing sagittal bend second mono crystal with 4:1 magnification ratio and vertically focusing mirror. |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→50 Å / Num. obs: 152700 / % possible obs: 75 % / Observed criterion σ(F): 2.046 / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.111 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2AQ3 Resolution: 2.6→50 Å / Cor.coef. Fo:Fc: 0.934 / Cor.coef. Fo:Fc free: 0.897 / SU B: 8.387 / SU ML: 0.184 / Cross valid method: THROUGHOUT / σ(F): 2 / ESU R Free: 0.268 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.069 Å2
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Refinement step | Cycle: LAST / Resolution: 2.6→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.667 Å / Total num. of bins used: 20
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