SUMO is transferred from E1 to E2 (UBE2I, UBC9) / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMOylation of nuclear envelope proteins / Vitamin D (calciferol) metabolism / Postmitotic nuclear pore complex (NPC) reformation / SUMOylation of ubiquitinylation proteins / SUMOylation of SUMOylation proteins / SUMOylation of DNA replication proteins / SUMOylation of transcription factors / SUMOylation of RNA binding proteins ...SUMO is transferred from E1 to E2 (UBE2I, UBC9) / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMOylation of nuclear envelope proteins / Vitamin D (calciferol) metabolism / Postmitotic nuclear pore complex (NPC) reformation / SUMOylation of ubiquitinylation proteins / SUMOylation of SUMOylation proteins / SUMOylation of DNA replication proteins / SUMOylation of transcription factors / SUMOylation of RNA binding proteins / SUMOylation of DNA methylation proteins / SUMOylation of DNA damage response and repair proteins / SUMOylation of transcription cofactors / SUMOylation of chromatin organization proteins / SUMOylation of intracellular receptors / SUMOylation of immune response proteins / Formation of Incision Complex in GG-NER / SUMO conjugating enzyme activity / Processing of DNA double-strand break ends / RING-like zinc finger domain binding / Transcriptional and post-translational regulation of MITF-M expression and activity / SUMO ligase complex / negative regulation of potassium ion transmembrane transporter activity / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / protein localization to nuclear pore / : / SUMOylation of nuclear envelope proteins / HLH domain binding / negative regulation of delayed rectifier potassium channel activity / SUMO is proteolytically processed / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMO is conjugated to E1 (UBA2:SAE1) / PML body organization / SUMO is transferred from E1 to E2 (UBE2I, UBC9) / nuclear stress granule / small protein activating enzyme binding / negative regulation of action potential / SUMOylation of DNA methylation proteins / SUMOylation of immune response proteins / XY body / SUMOylation of SUMOylation proteins / regulation of calcium ion transmembrane transport / Maturation of nucleoprotein / SUMOylation of RNA binding proteins / nuclear export / Transferases; Acyltransferases; Aminoacyltransferases / regulation of cardiac muscle cell contraction / Postmitotic nuclear pore complex (NPC) reformation / negative regulation of DNA binding / Maturation of nucleoprotein / cellular response to cadmium ion / negative regulation of protein import into nucleus / SUMOylation of ubiquitinylation proteins / transcription factor binding / ubiquitin-specific protease binding / ubiquitin-like protein ligase binding / roof of mouth development / SUMOylation of transcription factors / SUMOylation of DNA replication proteins / protein sumoylation / postsynaptic cytosol / potassium channel regulator activity / transporter activator activity / Regulation of IFNG signaling / nuclear pore / SUMOylation of DNA damage response and repair proteins / presynaptic cytosol / Transcriptional and post-translational regulation of MITF-M expression and activity / negative regulation of DNA-binding transcription factor activity / SUMOylation of transcription cofactors / SUMOylation of chromatin organization proteins / transcription coregulator binding / chromosome segregation / SUMOylation of intracellular receptors / positive regulation of protein-containing complex assembly / Formation of Incision Complex in GG-NER / protein tag activity / modulation of chemical synaptic transmission / protein modification process / PML body / PKR-mediated signaling / regulation of protein stability / Schaffer collateral - CA1 synapse / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / regulation of protein localization / cellular response to heat / nuclear membrane / positive regulation of canonical NF-kappaB signal transduction / protein stabilization / nuclear speck / nuclear body / positive regulation of cell migration / cell division / DNA repair / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / nucleolus / glutamatergic synapse / enzyme binding Similarity search - Function
Resolution: 2.22→61.31 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.874 / SU B: 11.002 / SU ML: 0.161 / Cross valid method: THROUGHOUT / ESU R: 0.355 / ESU R Free: 0.255 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS RESIDUES B94-B96 ARE DISORDERED AND HAVE BEEN GIVEN AN OCCUPANCY OF 0. THERE IS A COVALENT ISOPEPTIDE LINKAGE BETWEEN LYSINE A14 AND GLYCINE B97
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.256
547
4.7 %
RANDOM
Rwork
0.171
-
-
-
obs
0.175
11169
99.4 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK