+Open data
-Basic information
Entry | Database: PDB / ID: 2io2 | ||||||
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Title | Crystal structure of human Senp2 in complex with RanGAP1-SUMO-1 | ||||||
Components |
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Keywords | PROTEIN BINDING / HYDROLASE / SUMO / Ubiquitin / Senp / Ulp / complex | ||||||
Function / homology | Function and homology information SUMO-specific endopeptidase activity / cellular response to vasopressin / cytoplasmic periphery of the nuclear pore complex / SUMO ligase complex / negative regulation of transcription by transcription factor localization / SUMOylation of nuclear envelope proteins / deSUMOylase activity / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMO is proteolytically processed / negative regulation of delayed rectifier potassium channel activity ...SUMO-specific endopeptidase activity / cellular response to vasopressin / cytoplasmic periphery of the nuclear pore complex / SUMO ligase complex / negative regulation of transcription by transcription factor localization / SUMOylation of nuclear envelope proteins / deSUMOylase activity / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMO is proteolytically processed / negative regulation of delayed rectifier potassium channel activity / SUMO is conjugated to E1 (UBA2:SAE1) / protein desumoylation / nuclear stress granule / SUMO is transferred from E1 to E2 (UBE2I, UBC9) / nuclear pore cytoplasmic filaments / negative regulation of action potential / small protein activating enzyme binding / SUMOylation of DNA methylation proteins / SUMOylation of SUMOylation proteins / SUMOylation of immune response proteins / nuclear export / Maturation of nucleoprotein / Rev-mediated nuclear export of HIV RNA / activation of GTPase activity / negative regulation of protein export from nucleus / SUMOylation of RNA binding proteins / aggresome / regulation of Wnt signaling pathway / Postmitotic nuclear pore complex (NPC) reformation / nucleocytoplasmic transport / Maturation of nucleoprotein / negative regulation of protein import into nucleus / ubiquitin-specific protease binding / SUMOylation of ubiquitinylation proteins / roof of mouth development / fat cell differentiation / negative regulation of DNA binding / ubiquitin-like protein ligase binding / SUMOylation of DNA replication proteins / protein sumoylation / transcription factor binding / SUMOylation of transcription factors / mRNA transport / response to axon injury / potassium channel regulator activity / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / SUMOylation of DNA damage response and repair proteins / nuclear pore / Regulation of IFNG signaling / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / axon cytoplasm / negative regulation of protein ubiquitination / Resolution of Sister Chromatid Cohesion / cellular response to cadmium ion / SUMOylation of chromatin organization proteins / GTPase activator activity / SUMOylation of transcription cofactors / positive regulation of protein ubiquitination / RHO GTPases Activate Formins / positive regulation of protein-containing complex assembly / protein destabilization / SUMOylation of intracellular receptors / PKR-mediated signaling / negative regulation of DNA-binding transcription factor activity / PML body / mitotic spindle / protein tag activity / kinetochore / small GTPase binding / Wnt signaling pathway / Formation of Incision Complex in GG-NER / Separation of Sister Chromatids / regulation of protein localization / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / protein transport / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / cellular response to heat / nuclear envelope / nuclear membrane / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / protein stabilization / nuclear body / nuclear speck / cadherin binding / DNA repair / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / dendrite / ubiquitin protein ligase binding / nucleolus / perinuclear region of cytoplasm / enzyme binding / signal transduction / proteolysis / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | ||||||
Authors | Reverter, D. / Lima, C.D. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2006 Title: Structural basis for SENP2 protease interactions with SUMO precursors and conjugated substrates. Authors: Reverter, D. / Lima, C.D. | ||||||
History |
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Remark 300 | BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 3 ... BIOMOLECULE: 1 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT WHICH CONSISTS OF 3 CHAIN(S). SEE REMARK 350 FOR INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S). THERE IS A DOMAIN-SWAPPED DIMER ACROSS CRYSTALLOGRAPHIC TWO-FOLD INVOLVING CHAIN C, FORMED BY SYMMETRY OPERATION 7555 (Y,X,1/3-Z) |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2io2.cif.gz | 107.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2io2.ent.gz | 81.4 KB | Display | PDB format |
PDBx/mmJSON format | 2io2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/io/2io2 ftp://data.pdbj.org/pub/pdb/validation_reports/io/2io2 | HTTPS FTP |
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-Related structure data
Related structure data | 2io0C 2io1C 2io3C 1tgzS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 27342.648 Da / Num. of mol.: 1 / Fragment: catalytic domain / Mutation: C548S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SENP2, KIAA1331 / Plasmid: pET28b / Production host: Escherichia coli (E. coli) References: UniProt: Q9HC62, Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases |
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#2: Protein | Mass: 9473.775 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SUMO1, SMT3C, SMT3H3, UBL1 / Plasmid: pET28b / Production host: Escherichia coli (E. coli) / References: UniProt: P63165 |
#3: Protein | Mass: 18670.543 Da / Num. of mol.: 1 / Fragment: c-terminal domain / Mutation: C573S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RANGAP1 / Plasmid: pET28b / Production host: Escherichia coli (E. coli) / References: UniProt: P46060 |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.75 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 12% PEG 4000, 0.1M Lithium chloride, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.979 Å |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Aug 10, 2004 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→30 Å / Num. all: 14174 / Num. obs: 13707 / % possible obs: 96.7 % / Observed criterion σ(I): 1 / Redundancy: 13.5 % / Rmerge(I) obs: 0.071 / Χ2: 1.572 / Net I/σ(I): 25.8 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.292 / Mean I/σ(I) obs: 2.5 / Num. unique all: 1260 / Χ2: 0.691 / % possible all: 91.2 |
-Phasing
Phasing MR | Rfactor: 0.518 / Cor.coef. Fo:Fc: 0.376
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1TGZ Resolution: 2.9→14.94 Å / Rfactor Rfree error: 0.012 / FOM work R set: 0.671 / Data cutoff high absF: 3563397 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 10 Å2 / ksol: 0.216 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 90.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.9→14.94 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.9→3.08 Å / Rfactor Rfree error: 0.04 / Total num. of bins used: 6
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Xplor file |
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