SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA AB" IN EACH CHAIN ON SHEET RECORDS ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA AB" IN EACH CHAIN ON SHEET RECORDS BELOW ARE ACTUALLY 9-STRANDED BARRELS WHICH ARE REPRESENTED BY A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
RESIDUES ARE MISSING FROM N-TERMINUS OF THE CRYSTALLIZED SEQUENCE DUE TO CLONING STRATEGY AND POST- ...RESIDUES ARE MISSING FROM N-TERMINUS OF THE CRYSTALLIZED SEQUENCE DUE TO CLONING STRATEGY AND POST-TRANSLATIONAL PROCESSING. THE FIRST RESIDUE (G) OF THE CRYSTALLIZED PROTEIN IS A RESULT OF CLONING STRATEGY, TOO.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.31 Å3/Da / 溶媒含有率: 46.27 %
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: シンクロトロン / サイト: MAX II / ビームライン: I711 / 波長: 1.063
検出器
タイプ: MARRESEARCH / 検出器: CCD / 日付: 2005年4月21日
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.063 Å / 相対比: 1
反射
解像度: 1.95→25 Å / Num. obs: 20151 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / 冗長度: 11.9 % / Rmerge(I) obs: 0.01 / Net I/σ(I): 21
反射 シェル
解像度: 1.95→2.05 Å / 冗長度: 12 % / Rmerge(I) obs: 0.04 / Mean I/σ(I) obs: 6 / % possible all: 100
解像度: 1.95→25 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.923 / SU B: 3.999 / SU ML: 0.116 / 交差検証法: THROUGHOUT / ESU R: 0.17 / ESU R Free: 0.16 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THERE IS NCS WITHIN THE CHAIN A, SINCE THE CRYSTALLIZED STRUCTURE IS A PSEUDODIMER OF TWO CIRCULARLY PERMUTATED MONOMERS LINKED TOGETHER. ...詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THERE IS NCS WITHIN THE CHAIN A, SINCE THE CRYSTALLIZED STRUCTURE IS A PSEUDODIMER OF TWO CIRCULARLY PERMUTATED MONOMERS LINKED TOGETHER. HOWEVER, THE NCS IS COMPLICATED AND NOT INCLUDED.
Rfactor
反射数
%反射
Selection details
Rfree
0.246
1027
5.1 %
RANDOM
Rwork
0.198
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obs
0.2
19123
100 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK