SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA AB" IN EACH CHAIN ON SHEET RECORDS ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA AB" IN EACH CHAIN ON SHEET RECORDS BELOW ARE ACTUALLY 9-STRANDED BARRELS WHICH ARE REPRESENTED BY A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
Mass: 18.015 Da / Num. of mol.: 118 / Source method: isolated from a natural source / Formula: H2O
Sequence details
RESIDUES ARE MISSING FROM N-TERMINUS OF THE CRYSTALLIZED SEQUENCE DUE TO CLONING STRATEGY AND POST- ...RESIDUES ARE MISSING FROM N-TERMINUS OF THE CRYSTALLIZED SEQUENCE DUE TO CLONING STRATEGY AND POST-TRANSLATIONAL PROCESSING. THE FIRST RESIDUE (G) OF THE CRYSTALLIZED PROTEIN IS A RESULT OF CLONING STRATEGY, TOO.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.31 Å3/Da / Density % sol: 46.27 %
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: MAX II / Beamline: I711 / Wavelength: 1.063
Resolution: 1.95→25 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.923 / SU B: 3.999 / SU ML: 0.116 / Cross valid method: THROUGHOUT / ESU R: 0.17 / ESU R Free: 0.16 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THERE IS NCS WITHIN THE CHAIN A, SINCE THE CRYSTALLIZED STRUCTURE IS A PSEUDODIMER OF TWO CIRCULARLY PERMUTATED MONOMERS LINKED TOGETHER. ...Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THERE IS NCS WITHIN THE CHAIN A, SINCE THE CRYSTALLIZED STRUCTURE IS A PSEUDODIMER OF TWO CIRCULARLY PERMUTATED MONOMERS LINKED TOGETHER. HOWEVER, THE NCS IS COMPLICATED AND NOT INCLUDED.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.246
1027
5.1 %
RANDOM
Rwork
0.198
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obs
0.2
19123
100 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK