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Open data
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Basic information
Entry | Database: PDB / ID: 2adg | ||||||
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Title | Crystal structure of monoclonal anti-CD4 antibody Q425 | ||||||
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![]() | IMMUNE SYSTEM / anti-CD4 / interfacial metal / antibody recognition | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Zhou, T. / Hamer, D.H. / Hendrickson, W.A. / Sattentau, Q.J. / Kwong, P.D. | ||||||
![]() | ![]() Title: Interfacial metal and antibody recognition. Authors: Zhou, T. / Hamer, D.H. / Hendrickson, W.A. / Sattentau, Q.J. / Kwong, P.D. | ||||||
History |
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Remark 999 | SEQUENCE The sequence of FAB protein is not available at GB or SWS sequence database at the time of ...SEQUENCE The sequence of FAB protein is not available at GB or SWS sequence database at the time of processing. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 94.7 KB | Display | ![]() |
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PDB format | ![]() | 71.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2adiC ![]() 2adjC ![]() 1fvdS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | Heterodimer of heavy and light chain |
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Components
#1: Antibody | Mass: 23721.117 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Hybridoma / Source: (natural) ![]() ![]() |
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#2: Antibody | Mass: 23929.635 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Hybridoma / Source: (natural) ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.737 Å3/Da / Density % sol: 55.1 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: PEG4000, Sodium Acetate, EDTA, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Jan 20, 1994 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→10 Å / Num. all: 18319 / Num. obs: 18319 / % possible obs: 94.4 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.8 % / Biso Wilson estimate: 15.1 Å2 / Rsym value: 0.098 / Net I/σ(I): 7.3 |
Reflection shell | Resolution: 2.5→2.61 Å / % possible all: 78.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 1FVD Resolution: 2.5→10 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 62726.54 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 30.361 Å2 / ksol: 0.316614 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.5→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.66 Å / Rfactor Rfree error: 0.03 / Total num. of bins used: 6
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Xplor file |
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