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Yorodumi- PDB-1qt1: CRYSTAL STRUCTURE OF XYLOSE ISOMERASE FROM STREPTOMYCES DIASTATIC... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1qt1 | ||||||
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Title | CRYSTAL STRUCTURE OF XYLOSE ISOMERASE FROM STREPTOMYCES DIASTATICUS NO.7 M1033 AT 1.85 A RESOLUTION | ||||||
Components | PROTEIN (XYLOSE ISOMERASE) | ||||||
Keywords | ISOMERASE / XYLOSE ISOMERASE / GLUCOSE ISOMERASE / STREPTOMYCES / TRUE SPACE GROUP | ||||||
Function / homology | Function and homology information xylose isomerase / D-xylose metabolic process / xylose isomerase activity / magnesium ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Streptomyces diastaticus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.85 Å | ||||||
Authors | Niu, L. / Teng, M. / Zhu, X. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2000 Title: Structure of xylose isomerase from Streptomyces diastaticus no. 7 strain M1033 at 1.85 A resolution. Authors: Zhu, X. / Teng, M. / Niu, L. / Xu, C. / Wang, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1qt1.cif.gz | 174.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1qt1.ent.gz | 135.4 KB | Display | PDB format |
PDBx/mmJSON format | 1qt1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qt/1qt1 ftp://data.pdbj.org/pub/pdb/validation_reports/qt/1qt1 | HTTPS FTP |
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-Related structure data
Related structure data | 1clkSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.999116, -0.042047, 0.000169), Vector: Details | THE MOLECULE IS A TETRAMER IN THE CRYSTAL CONTAINING TWO SUBUNITS IN THE ASYMMETRIC UNIT WHICH WERE REFINED INDEPENDENTLY. THE FIRST SUBUNIT IS NUMBERED FROM A 1 - A 387 AND THE SECOND SUBUNIT IS NUMBERED FROM B 501 - B 887. THE SUBUNITS ARE RELATED BY AN APPROXIMATE SYMMETRY. THE TRANSFORMATION PRESENTED ON *MTRIX* RECORDS BELOW WILL YIELD APPROXIMATE COORDINATES FOR CHAIN B WHEN APPLIED TO CHAIN A. | |
-Components
#1: Protein | Mass: 42695.641 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Streptomyces diastaticus (bacteria) / Strain: M1033 / References: UniProt: P50910, xylose isomerase #2: Chemical | ChemComp-CO / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.53 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.5418 |
Detector | Type: SIEMENS / Detector: AREA DETECTOR |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→5 Å / Num. obs: 55255 / % possible obs: 82.78 % / Redundancy: 4 % / Rmerge(I) obs: 0.085 / Net I/σ(I): 10.95 |
Reflection shell | Resolution: 1.85→1.93 Å / Mean I/σ(I) obs: 3.45 / % possible all: 72.83 |
Reflection shell | *PLUS % possible obs: 72.8 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1CLK Resolution: 1.85→5 Å / Cross valid method: THROUGHOUT / σ(F): 2
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Displacement parameters | Biso mean: 12.54 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.85→5 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 1.85→1.93 Å / Total num. of bins used: 8
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Xplor file | Serial no: 1 / Param file: PARHCSDX.PRO OF X-PLOR3.1 / Topol file: TOPHCSDX.PRO OF X-PLOR3.1 |